2019 Targeting Trends Review

Katsurada K, Nakata M, Saito T, Zhang B, Maejima Y, Nandi SS, Sharma NM, Patel KP, Kario K, Yada T (2019) Central glucagon-like peptide-1 receptor signaling via brainstem catecholamine neurons counteracts hypertension in spontaneously hypertensive rats. Sci Rep 9(1):12986. doi: 10.1038/s41598-019-49364-x

Objective: To determine mechanisms for antihypertensive effect of GLP-1R agonists.

Summary: The central GLP-1R signaling via NTS DBH neurons counteracts the development of hypertension in SHR, accompanied by attenuated sympathetic nerve activity.

Usage: Anti-DBH-SAP or Blank-SAP was injected into NTS bilaterally (6 ng/200 nl).

Related Products: Anti-DBH-SAP (Cat. #IT-03), Blank-SAP (Cat. #IT-21)

Zhuo X, Wu Y, Yang Y, Gao L, Qiao X, Chen T (2019) LncRNA AK094457 promotes AngII-mediated hypertension and endothelial dysfunction through suppressing of activation of PPARγ. Life Sci 233:116745. doi: 10.1016/j.lfs.2019.116745 PMID: 31404524

Objective: To find the functional lncRNAs associated with hypertension, high throughput lncRNA microarray were used to analyze expression profile of the lncRNAs in the aortic vascular endothelial cells of spontaneously hypertensive rats.

Summary: lncRNA AK094457 is identified as a key regulator in blood pressure and endothelial function, which can increase AngII-induced hypertension and endothelial dysfunction via suppression of PPARγ.

Usage: Western Blot (1:500), immunohistochemistry

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Veening-Griffioen DH, Ferreira GS, van Meer PJK, Boon WPC, Gispen-de Wied CC, Moors EHM, Schellekens H (2019) Are some animal models more equal than others? A case study on the translational value of animal models of efficacy for Alzheimer’s disease. Eur J Pharmacol 859:172524. doi: 10.1016/j.ejphar.2019.172524 PMID: 31291566

Related Products: 192-IgG-SAP (Cat. #IT-01)

Bülbül M, Sinen O, Bayramoğlu O, Akkoyunlu G (2019) Enteric apelin enhances the stress-induced stimulation of colonic motor functions. Stress 23(2):201-212. doi: 10.1080/10253890.2019.1658739 PMID: 31441348

Usage: histology (1:100)

Related Products: Corticotropin Releasing Hormone Rabbit Polyclonal (Cat. #AB-02)

Ladd MR, Costello CM, Gosztyla C, Werts AD, Johnson B, Fulton WB, Martin LY, Redfield EJ, Crawford B, Panaparambil R, Sodhi CP, March JC, Hackam DJ (2019) Development of intestinal scaffolds that mimic native mammalian intestinal tissue. Tissue Eng Part A 25(17-18):1225-1241. doi: 10.1089/ten.TEA.2018.0239 PMID: 30652526

Objective: To develop a scaffold for the generation of an artificial intestine that specifically mimics the architecture and biomechanical properties of the native small intestine, and to evaluate the scaffold in vitro and in vivo.

Summary: Novel scaffolds were developed using PGS with promise for use in artificial intestine for individuals with short bowel syndrome.

Usage: Histology, immunohistochemistry, microscopy (1:500)

Related Products: NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01)

Saw S, Aiken A, Fang H, McKee TD, Bregant S, Sanchez O, Chen Y, Weiss A, Dickson BC, Czarny B, Sinha A, Fosang A, Dive V, Waterhouse PD, Kislinger T, Khokha R (2019) Metalloprotease inhibitor timp proteins control FGF-2 bioavailability and regulate skeletal growth. J Cell Biol 218(9):3134-3152. doi: 10.1083/jcb.201906059 PMID: 31371388

Usage: immunostaining (1:100)

Related Products: Fibroblast Growth Factor Rabbit Polyclonal, mammalian (Cat. #AB-07)

Jenrette TA, Logue JB, Horner KA (2019) Lesions of the patch compartment of dorsolateral striatum disrupt stimulus-response learning. Neuroscience 415:161-172. doi: 10.1016/j.neuroscience.2019.07.033

Objective: To investigate whether enhanced activation of the patch compartment contributes to habitual behavior.

Summary: The dorsolateral patch compartment may mediate habit formation by altering information flow through basal ganglia circuits.

Usage: A volume of 2 ul of Dermorphin-SAP (17 ng/ul or an equivalent amount of unconjugated SAP (as a control) was infused bilaterally, at a rate of 0.5 ul/min.

Related Products: Dermorphin-SAP / MOR-SAP (Cat. #IT-12), Saporin (Cat. #PR-01)

Ritter S, Li A-J, Wang Q (2019) Hindbrain glucoregulatory mechanisms: Critical role of catecholamine neurons in the ventrolateral medulla. Physiol Behav 208:112568. doi: 10.1016/j.physbeh.2019.112568

Objective: To explore circuitry and potential glucose-sensing mechanisms that contribute to the functions of glucoregulatory catecholamine neurons in the ventrolateral medulla

Summary: Selective lesion of hindbrain catecholamine neurons abolishes glucoprivic elicitation of key counterregulatory responses. Selective chemogenetic activation of specific catecholamine populations elicits these responses.

Related Products: Anti-DBH-SAP (Cat. #IT-03), NPY-SAP (Cat. #IT-28)

Soares JI, Da Costa C, Ferreira MH, Andrade PA, Maia GH, Lukoyanov NV (2019) Partial depletion of septohippocampal cholinergic cells reduces seizure susceptibility, but does not mitigate hippocampal neurodegeneration in the kainate model of epilepsy. Brain Res 1717:235-246. doi: 10.1016/j.brainres.2019.04.027

Objective: To examine how the inhibition of epilepsy-related cholinergic plasticity may be reflected in seizure susceptibility and/or in the development of chronic epilepsy and its neurological consequences.

Summary: These data suggest that seizure-induced plasticity of cholinergic cells may indeed enhance seizure susceptibility and contribute to epileptogenic processes. They do not support the hypothesis that epilepsy-related hypertrophy of cholinergic neurons may potentiate hippocampal cell loss and respective behavioral impairments.

Usage: Bilateral lesions of cholinergic cells were made by infusing 0.5 μl of 192-IgG-saporin (0.08 μg/μl saline solution) into the hippocampus.

Related Products: 192-IgG-SAP (Cat. #IT-01)

Maejima Y, Yokota S, Horita S, Shimomura K (2019) The undeveloped properties of GABA neurons in the ventral tegmental area promote energy intake for growth in juvenile rats. Sci Rep 9(1):11848. doi: 10.1038/s41598-019-48336-5

Objective: To determine the underlying mechanisms that induce high energy intake (EI) per body weight (BW).

Summary: Undeveloped properties of VTA GABA neurons in juvenile rats can promote higher EI regardless of high or less palatable feeding, and contribute to growth promotion.

Usage: GAT1-SAP or control, Rabbit IgG-SAP, was bilaterally injected (0.025 μg/0.5 μl) into the VTA in eight-week-old adult rats.

Related Products: GAT1-SAP (Cat. #IT-32), Rabbit IgG-SAP (Cat. #IT-35)