antibodies

Zhang C, Shen L, Zhu Y, Xu R, Deng Z, Liu X, Ding Y, Wang C, Shi Y, Bei L, Wei D, Thorne RF, Zhang XD, Yu L, Chen S (2021) KDM6A promotes imatinib resistance through YY1-mediated transcriptional upregulation of TRKA independently of its demethylase activity in chronic myelogenous leukemia. Theranostics 11(6):2691-2705. doi: 10.7150/thno.50571 PMID: 33456567

Objective: To identify histone lysine demethylases (KDMs) with altered expression in chronic myelogenous leukemia (CML) and define their contribution to imatinib resistance.

Summary: KDM6A promotes imatinib-resistance in CML cells. The identification of the KDM6A/YY1/TRKA axis as a novel imatinib-resistance mechanism represents an unexplored avenue to overcome tyrosine kinase inhibitor resistance in CML.

Usage: Western blot

Related Products: trkA Rabbit Polyclonal (Cat. #AB-N03)

Ringel AE, Drijvers JM, Baker GJ, Catozzi A, García-Cañaveras JC, Gassaway BM, Miller BC, Juneja VR, Nguyen TH, Joshi S, Yao CH, Yoon H, Sage PT, LaFleur MW, Trombley JD, Jacobson CA, Maliga Z, Gygi SP, Sorger PK, Rabinowitz JD, Sharpe AH, Haigis MC (2020) Obesity shapes metabolism in the tumor microenvironment to suppress anti-tumor immunity. Cell 183(7):1848-1866.e26. doi: 10.1016/j.cell.2020.11.009 PMID: 33301708

Objective: To investigate how obesity shifts the metabolic landscape of the tumor microenvironment (TME) to inhibit T cell function and promote tumor growth.

Summary: High-fat diet – induced obesity impairs CD8+ T cell function in the murine TME, accelerating tumor growth.

Usage: Immunoprecipitation

Related Products: Trans-4-Hydroxy-L-Proline Rabbit Polyclonal, Conjugated (Cat. #AB-T044)

Uda M, Yoshihara T, Ichinoseki-Sekine N, Baba T, Yoshioka T (2020) Potential roles of neuronal nitric oxide synthase and the PTEN-induced kinase 1 (PINK1)/Parkin pathway for mitochondrial protein degradation in disuse-induced soleus muscle atrophy in adult rats. PLoS One 15(12):e0243660. doi: 10.1371/journal.pone.0243660 PMID: 33296434

Objective: To investigate the involvement of neuronal nitric oxide synthase (nNOS) and the PINK1/Parkin pathway in soleus muscle atrophy induced by 14 days of hindlimb unloading (HU) in adult rats.

Summary: Excessive NO is not produced in atrophied soleus muscles despite nNOS accumulation, suggesting that excessive NO dose not mediate in soleus muscle atrophy at least after 14 days of HU.

Usage: Western blot (1:1000)

Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)

Motohashi T, Kawamura N, Watanabe N, Kitagawa D, Goshima N, Kunisada T (2020) Sox10 functions as an inducer of the direct conversion of keratinocytes into neural crest cells. Stem Cells Dev 29(23):1510-1519. doi: 10.1089/scd.2020.0106 PMID: 33040687

Objective: To investigate whether Sox10 functions in the direct conversion of other somatic cells into neural crest cells (NCCs).

Summary: Results demonstrate that Sox10 functions as an inducer of direct conversion into NCCs in cooperation with the transcription factors involved in NCC generation.

Usage: Flow cytometry and cell sorting

Related Products: NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01)

Bragina ME, Costa-Fraga F, Sturny M, Ebadi B, Ruoccolo RT, Santos RAS, Fraga-Silva RA, Stergiopulos N (2020) Characterization of the renin-angiotensin system in aged cavernosal tissue and its role in penile fibrosis. J Sex Med 17:2129–2140. doi: 10.1016/j.jsxm.2020.08.008 PMID: 32943375

Objective: To investigate local regulation of the renin-angiotensin system (RAS) in age-related erectile dysfunction (ED).

Summary: Experimental results highlighted the essential role of the RAS in modulation of cavernosal fibrosis.

Usage: Immunohistochemistry (1:800)

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Wang Z, Wu J, Hu Z, Luo C, Wang P, Zhang Y, Li H (2020) Dexmedetomidine alleviates lipopolysaccharide-induced acute kidney injury by inhibiting p75NTR-mediated oxidative stress and apoptosis. Oxid Med Cell Longev 2020:5454210. doi: 10.1155/2020/5454210 PMID: 33194004

Objective: To study antioxidant effects and the mechanism of Dexmedetomidine (DEX) in an inflammatory proximal tubular epithelial cell model and lipopolysaccharide- (LPS-) induced AKI in mice.

Summary: DEX ameliorated AKI in mice with sepsis by partially reducing oxidative stress and apoptosis through regulation of p75NTR/p38MAPK/JNK signaling pathways.

Usage: Western blot (1:5000); AB-N01AP: NGFr (mu p75) Rabbit Polyclonal

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Kim HY, Kim HS (2021) Sulfatase 1 mediates IL-10-induced dimethylarginine dimethylaminohydrolase-1 expression and antiproliferative effects in vascular smooth muscle cells of spontaneously hypertensive rats. Cytokine 137:155344. doi: 10.1016/j.cyto.2020.155344 PMID: 33128921

Objective: To examine the effects of exSulfs on IL-10-induced dimethylarginine dimethylaminohydrolase-1 (DDAH-1) expression, abrogation of Ang II-induced DDAH-1 downregulation, and inhibition of Ang II-induced proliferation of spontaneously hypertensive rats (SHRs) vascular smooth muscle cells (VSMC). IL-10-induced DDAH-1 expression and abrogation of Ang II-induced DDAH-1 downregulation were attenuated in Sulf1 siRNA-transfected SHRs VSMC.

Summary: Sulf1, and not Sulf2, mediates the IL-10-induced inhibition of Ang II-induced hypertensive effects in SHRs VSMC.

Usage: Western blot (1:800)

Related Products: Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

Cassandras M, Wang C, Kathiriya J, Tsukui T, Matatia P, Matthay M, Wolters P, Molofsky A, Sheppard D, Chapman H, Peng T (2020) Gli1+ mesenchymal stromal cells form a pathological niche to promote airway progenitor metaplasia in the fibrotic lung. Nat Cell Biol 22(11):1295-1306. doi: 10.1038/s41556-020-00591-9 PMID: 33046884

Objective: To show that Gli1+ mesenchymal stromal cells (MSCs) promote metaplastic differentiation of airway progenitors into KRT5+ basal cells.

Summary: Gli1+ MSCs integrate hedgehog signaling as a rheostat to control BMP activation in the progenitor niche to determine regenerative outcome in fibrosis.

Usage: Histology and Immunofluorescence (1:200)

Related Products: NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01)

Cops J, De Moor B, Haesen S, Lijnen L, Wens I, Lemoine L, Reynders C, Penders J, Lambrichts I, Mullens W, Hansen D (2020) Endurance exercise intervention is beneficial to kidney function in a rat model of isolated abdominal venous congestion: A pilot study. J Cardiovasc Transl Res 13(5):769-782. doi: 10.1007/s12265-019-09947-5 PMID: 31848881

Objective: To the effects of moderate intense endurance exercise on heart and kidney function and morphology in a rat model of abdominal venous congestion.

Summary: The implementation of moderate intense endurance exercise reduces abdominal venous pressure and is beneficial to kidney function.

Usage: estern blot; to investigate whole kidney renin-angiotensin-aldosterone system (RAAS) protein expression, the membrane was incubated overnight at 4 °C in the presence of AT-1R antibody 1:2000

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Feng X, Guo Q, Xue H, Duan X, Jin S, Wu Y (2020) Hydrogen sulfide attenuated Angiotensin II-induced sympathetic excitation in offspring of renovascular hypertensive rats. Front Pharmacol 11:565726. doi: 10.3389/fphar.2020.565726 PMID: 33041805

Objective: To whether sensitivity to angiotensin II (Ang II) is enhanced in offspring of renovascular hypertensive animals and whether hydrogen sulfide (H2S) can attenuate the increased response to Ang II in offspring.

Summary: Prenatal or postnatal administration of H2S attenuated AngII-induced sympathetic excitation in offspring of hypertensive rats.

Usage: Western blot (1:800)

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)