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Infrared fluorescence imaging of infarcted hearts with Ag2S nanodots.
Ortgies DH, García-Villalón ÁL, Granado M, Amor S, Rodríguez EM, Santos HDA, Yao J, Rubio-Retama J, Jaque D (2019) Infrared fluorescence imaging of infarcted hearts with Ag2S nanodots. Nano Res 12:749–757. doi: 10.1007/s12274-019-2280-4 PMID: 0
Objective: To investigate the potential application of functionalized Ag2S nanodots for accurate imaging of damaged myocardium tissues after a myocardial infarction induced by either partial or global ischemia.
Summary: NIR-II images generated by ATII-functionalized Ag2S nanodots contain valuable information about the location and extension of damaged tissue in the myocardium allowing for a proper identification of the occluded artery as well as an indirect evaluation of the damage level.
Usage: Assessment of AT1R protein content in ischemic and non-ischemic hearts by Western blot (1:1000).
Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)
Effects of Shugan Hewei Granule on depressive behavior and protein expression related to visceral sensitivity in a rat model of nonerosive reflux disease.
Wang Y, Li G, Wang X, Zhu S (2019) Effects of Shugan Hewei Granule on depressive behavior and protein expression related to visceral sensitivity in a rat model of nonerosive reflux disease. Evid Based Complement Alternat Med 2019:1505693. doi: 10.1155/2019/1505693 PMID: 30719054
Usage: immunofluorescent staining (1:200)
Related Products: Corticotropin Releasing Hormone Rabbit Polyclonal (Cat. #AB-02)
Expression of transcription factors divides retinal ganglion cells into distinct classes.
Sweeney NT, James KN, Nistorica A, Lorig-Roach RM, Feldheim DA (2019) Expression of transcription factors divides retinal ganglion cells into distinct classes. J Comp Neurol 527(1):225-235. doi: 10.1002/cne.24172 PMID: 28078709
Usage: Melanopsin (Opn4) Immunostaining 1:1000
Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)
The M6 cell: A small-field bistratified photosensitive retinal ganglion cell.
Quattrochi LE, Stabio ME, Kim I, Ilardi MC, Michelle Fogerson P, Leyrer ML, Berson DM (2019) The M6 cell: A small-field bistratified photosensitive retinal ganglion cell. J Comp Neurol 527(1):297-311. doi: 10.1002/cne.24556 PMID: 30311650
Summary: M6 cells express low levels of melanopsin and have correspondingly weak intrinsic light responses.
Usage: In all experiments involving melanopsin immunofluorescence, melanopsin immunodetection was enhanced using tyramide signal amplification coupled with horseradish peroxidase (HRP)-paired with goat anti-rabbit IgG and an Alexa fluorophore. 1:10,000.
Related Products: Melanopsin Rabbit Polyclonal (Cat. #AB-N38)
Assembly of functionally antagonistic visual circuits for controlling pupil dynamics
Dhande OS, Seabrook TA, Phan AH, Salaly LD, Ishiko N, Nguyen PL, Wang JT, Evans SM, Huberman AD (2018) Assembly of functionally antagonistic visual circuits for controlling pupil dynamics. bioRxiv 500868. doi: 10.1101/500868
Objective: Investigate the mechanisms controlling the specification and establishment of parallel sensory pathways.
Summary: Identification of a novel genetic program that marks and is required for the development of non-image-forming parallel visual pathways.
Usage: Immunohistochemistry (1:1000)
Related Products: Melanopsin Rabbit Polyclonal, affinity-purified (Cat. #AB-N39)
Selective abdominal venous congestion induces adverse renal and hepatic morphological and functional alterations despite a preserved cardiac function
Cops J, Mullens W, Verbrugge FH, Swennen Q, De Moor B (2018) Selective abdominal venous congestion induces adverse renal and hepatic morphological and functional alterations despite a preserved cardiac function. Sci Rep 8:17757. doi: 10.1038/s41598-018-36189-3 PMID: 30532057
Objective: The consequences of isolated abdominal venous congestion on morphology and function of the kidneys, liver and heart were studied in a rat model.
Summary: This study provides relevant insight in the pathophysiology of abdominal congestion on organ function.
Usage: Western blot. PVDF membrane was incubated overnight at 4 °C in the presence of antibody (1/2000).
Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)
Immunological properties of neural crest cells derived from human induced pluripotent stem cells.
Fujii S, Yoshida S, Inagaki E, Hatou S, Tsubota K, Takahashi M, Shimmura S, Sugita S (2019) Immunological properties of neural crest cells derived from human induced pluripotent stem cells. Stem Cells Dev 28(1):28-43. doi: 10.1089/scd.2018.0058 PMID: 30251915
Objective: To assess the immunological properties of human induced pluripotent stem cells derived neural crest cells (iPSC-NCCs)
Summary: Cultured human NCCs can fully suppress T cell activation in vitro and may be used in cell-based medicine.
Usage: IHC; 1:200 dilution
Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)
Single quantum dot tracking reveals serotonin transporter diffusion dynamics are correlated with cholesterol-sensitive threonine 276 phosphorylation status in primary midbrain neurons.
Bailey D, Catron M, Kovtun O, Macdonald R, Zhang Q, Rosenthal S (2018) Single quantum dot tracking reveals serotonin transporter diffusion dynamics are correlated with cholesterol-sensitive threonine 276 phosphorylation status in primary midbrain neurons. ACS Chem Neurosci 9:2534-2541. doi: 10.1021/acschemneuro.8b00214 PMID: 29787674
Objective: Visualizing SERT behavior at the single molecule level in endogenous systems remains a challenge. The authors utilize quantum dot (QD) single particle tracking (SPT) to capture SERT dynamics in primary rat midbrain neurons.
Summary: Results provide new insights into endogenous neuronal SERT mobility and its associations with membrane cholesterol and SERT phosphorylation status.
Usage: Live Cell QD Tracking – Cells were incubated with 5 μg/mL primary SERT antibody in warm fluorescent media at 37 °C and 5% CO2 for 6 min.
Related Products: SERT Mouse Monoclonal (Cat. #AB-N40)
Effectiveness of topical administration of Anethum graveolens essential oil on MRSA-infected wounds
Manzuoerh R, Farahpour MR, Oryan A, Sonboli A (2019) Effectiveness of topical administration of Anethum graveolens essential oil on MRSA-infected wounds. Biomed Pharmacother 109:1650-1658. doi: 10.1016/j.biopha.2018.10.117 PMID: 30551419
Usage: immunohistochemistry (1:500)
Related Products: Fibroblast Growth Factor Rabbit Polyclonal, mammalian (Cat. #AB-07)
Selective laminin-directed differentiation of human induced pluripotent stem cells into distinct ocular lineages
Shibata S, Hayashi R, Okubo T, Kudo Y, Katayama T, Ishikawa Y, Toga J, Yagi E, Honma Y, Quantock AJ, Sekiguchi K, Nishida K (2018) Selective laminin-directed differentiation of human induced pluripotent stem cells into distinct ocular lineages. Cell Rep 25:1668-1679. doi: 10.1016/j.celrep.2018.10.032 PMID: 30404017
Usage: Immunostaining, flow cytometry
Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)