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A specific cholinergic immunotoxin in mice.
Berger-Sweeney JE, Murg SL, Baxter MG, Stearns NA, Lappi DA (2000) A specific cholinergic immunotoxin in mice. Neuroscience 2000 Abstracts 563.13. Society for Neuroscience, New Orleans, LA.
Summary: We have shown previously that 192 IgG-saporin, a p75 (nerve growth factor [NGF] receptor) antibody linked to the ribosome-inactivating protein saporin, is an effective lesioning agent for cholinergic basal forebrain neurons in rats (Berger-Sweeney et al., J. Neurosci. 14:4507-4519). The 192 IgG antibody, however, does not crossreact with the mouse NGF receptor, making it unsuitable for mouse studies. Here, we tested the efficacy of a new immunotoxin targeting the mouse p75 receptor. A rat monoclonal antibody (Advanced Targeting Systems) to the extracellular domain of the mouse p75 receptor, which can be internalized and transported retrogradely in mouse neurons (Rossner et al., Metab. Brain Dis. 15:17-28), was coupled to saporin. Different doses of the toxin, or saline (0.5 µl) were injected into the lateral ventricle (icv) of adult C57BL/6 mice (n = 3–6/ group). Two weeks later, brains were processed for choline acetyltransferase (ChAT) neurochemistry or ChAT immunocytochemistry (to examine lesion efficacy), and glutamic acid decarboxylase (GAD) neurochemistry or calbindin/parvalbumin immunocytochemistry (to examine lesion specificity). Toxin doses ranging from 0.4 – 3.6 µg reduced hippocampal and neocortical ChAT activity in a dose-dependent fashion. Immunocytochemistry confirmed a significant loss of ChAT-positive neurons in the basal forebrain. These same doses did not alter hippocampal or neocortical GAD activity, or alter calbindin or parvalbumin staining (non-cholinergic neurons) in the basal forebrain. These data suggest that we have created a specific cholinergic immunotoxin for use in mice.
Related Products: mu p75-SAP (Cat. #IT-16)
Intracerebroventricular infusion of CHO5, a rat monoclonal antibody directed against mouse low-affinity nerve growth factor receptor (p75NTR), specifically labels basal forebrain cholinergic neurons in mouse brain.
Rossner S, Schliebs R, Bigl V (2000) Intracerebroventricular infusion of CHO5, a rat monoclonal antibody directed against mouse low-affinity nerve growth factor receptor (p75NTR), specifically labels basal forebrain cholinergic neurons in mouse brain. Metab Brain Dis 15(1):17-27. doi: 10.1007/BF02680011 PMID: 10885538
Summary: 192-Saporin (Cat. #IT-01) has long been a useful tool for neurobiological research in the rat. For various reasons, many researchers want to perform the same studies in the mouse but have been prevented from doing so by the lack of a suitable antibody against the mouse p75NTR. Rossner et al. describe a rat monoclonal antibody against the mouse p75NTR (Cat. #AB-N02) that demonstrates co-localization of p75NTR and ChAT, and also co-localization of p75NTR and TrkA in the mouse basal forebrain. Internalization and retrograde transport of this antibody in cholinergic basal forebrain neurons is also shown. This evidence indicates that the anti-mouse p75NTR will be effective for use as an immunotoxin.
Related Products: 192-IgG-SAP (Cat. #IT-01), mu p75-SAP (Cat. #IT-16), Antibody to NGFR (Cat. #AB-N02)