Yu X, Liu Z, Hou R, Nie Y, Chen R (2017) Nerve growth factor and its receptors on onset and diagnosis of ovarian cancer. Oncol Lett 14(3):2864-2868. doi: 10.3892/ol.2017.6527 PMID: 28928825

Objective: To investigate the effect of nerve growth factor (NGF) and its receptors on the onset and diagnosis of ovarian cancer.

Summary: NGF and its receptor can contribute to the occurrence of ovarian cancer, and the onset condition of ovarian cancer can be diagnosed through the detection of high or low expression of NGF and its receptors.

Usage: ELISA, WB, IHC (1:500)

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Wang G, Ma Z, Cao L, Yan G, Wang Y, Jin Y, Shen H, Zhang Y, Xu X, Chen X, Shen Z (2017) A novel method for obtaining highly enriched Schwann cell populations from mature monkey nerves based on in vitro pre‑degeneration. Mol Med Rep 16(5):6600-6607. doi: 10.3892/mmr.2017.7427 PMID: 28901508

Objective: To identify a novel technique that can efficiently isolate and purify Schwann cells (SCs) from mature monkey nerves based on in vitro pre-degeneration.

Summary: Based on their observations, the migration rate of SCs out of the nerve explants appeared to reach its maximum after 14 days. However, the level of fibroblast proliferation was also significantly increased, which reduced the purity of the SCs. Therefore, the shorter 7-day pre-degeneration period may be optimal to maximize SC purity. These Schwann cells may be used to provide support for regenerating peripheral or central nerves.

Usage: Immunofluorescence (1:500) and flow cytometry

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Falade TE, Olude MA, Mustapha OA, Mbajiorgu EF, Ihunwo AO, Olopade JO, Oke BO (2017) Connective tissue, glial and neuronal expressions in testis of the African giant rat (Cricetomys gambianus). J Morphological Sciences 34(03):186-193. doi: 10.4322/jms.109117

Objective: To investigate the expression of connective tissue (Collagens I and III), glia and neuronal markers in the testis of the African giant rat.

Summary: The cyto-architecture of the testis was typical in the African giant rat. The connective tissue expressed in the juvenile and adult group, signaling of glial-like cells were seen in the perivascular region across the experimental groups. Immuno-localization of neuronal cells was seen in the interstitial spaces across all the groups, but with more expressions in the juvenile.

Usage: IHC (1:700)

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Brick, R (2017) Induced pluripotent stem cell-derived mesenchymal progenitor cells: Promotion of neuritogenesis and axon elongation through neurotrophin and cytokine production. University of Pittsburgh Thesis.

Objective: To test if mesenchymal progenitor cells (MiMPCs), originally derived from mesenchymal stem cells (MSCs), have the same ability to support neuronal regeneration as the parent MSC.

Summary: Results show that MiMPCs can secrete neurotrophic factors after neuroinductive treatment and can produce factors to improve neurite outgrowth in a chick embryonic dorsal root ganglion (DRG) model. These findings suggest that neurotrophically induced-MiMPCs (NI-MiMPCs) may be considered a suitable substitute cell type to support nerve growth.

Usage: immunofluorescence

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Lai FP, Lau ST, Wong JK, Gui H, Wang RX, Zhou T, Lai WH, Tse HF, Tam PK, Garcia-Barcelo MM, Ngan ES (2017) Correction of Hirschsprung-associated mutations in human induced pluripotent stem cells via clustered regularly interspaced short palindromic repeats/cas9, restores neural crest cell function. Gastroenterology 153(1):139-153.e8. doi: 10.1053/j.gastro.2017.03.014 PMID: 28342760

Objective: To identify mutations associated with short-segment Hirschsprung disease (S-HSCR), and use the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 gene editing system to determine how mutations affect enteric neural crest cells (ENCC) function.

Summary: Mutations in vinculin gene (VCL) associated with S-HSCR were identified. Correction of this mutation in iPSC using CRISPR/Cas9 editing, as well as the RET G731del mutation that causes Hirschsprung disease with total colonic aganglionosis, restored ENCC function. The study demonstrates how human iPSCs can be used to identify disease-associated mutations and determine how they affect cell functions and contribute to pathogenesis.

Usage: Immunofluorescence (1:500)

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Yao P, Ding Y, Han Z, Mu Y, Hong T, Zhu Y, Li H (2017) Suppression of asparaginyl endopeptidase attenuates breast cancer-induced bone pain through inhibition of neurotrophin receptors. Mol Pain 13:744806917708127. doi: 10.1177/1744806917708127 PMID: 28554249

Objective: To determine the functions and targeted suppression of asparaginyl endopeptidase (AEP) in a mouse model of breast cancer-induced bone pain.

Summary: Targeted suppression of AEP with specific small compounds significantly reduced bone pain while purified recombinant AEP proteins increased bone pain. AEP aggravates the development of breast cancer bone metastasis and bone pain by increasing the expression of neurotrophin receptors. AEP might be an effective target for treatment of breast cancer-induced bone pain.

Usage: Western blot

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Wu W, Wu XL, Ji YQ, Gao Z (2017) Differentiation of nestin‑negative human hair follicle outer root sheath cells into neurons in vitro. Mol Med Rep 16(1):95-100. doi: 10.3892/mmr.2017.6585 PMID: 28534946

Objective: To isolate nestin-negative outer root sheath cells (ORSCs) from adult healthy volunteers and investigate the neuronal differentiation of these cells.

Summary: The present study did not verify the value of neural differentiation into sphere-forming cells for therapeutic application, however, the differentiation of nestin-negative human hair follicle ORSCs into neurons in vitro may serve as a theoretical underpinning for neurodegenerative diseases and regenerative medicine.

Usage: IF (1:250)

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Dorard C, Estrada C, Barbotin C, Larcher M, Garancher A, Leloup J, Beermann F, Baccarini M, Pouponnot C, Larue L, Eychène A, Druillennec S (2017) RAF proteins exert both specific and compensatory functions during tumour progression of NRAS-driven melanoma. Nat Commun 8:15262. doi: 10.1038/ncomms15262 PMID: 28497782

Objective: To investigate the contribution of Raf genes in tumor progression, from the onset of benign tumors to malignant tumor maintenance, in NRAS-induced mouse melanoma models

Summary: BRAF expression is required for ERK activation and nevi development, demonstrating a critical role in the early stages of NRAS-driven melanoma. After melanoma formation, single BRAF or CRAF ablation is not sufficient to block tumor growth, showing redundant functions for RAF kinases. Proliferation of resistant cells emerging in the absence of BRAF and CRAF remains dependent on ARAF-mediated ERK activation.

Usage: Cell sorting by Flow Cytometry (1:500)

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Guo JR, Wang H, Jin XJ, Jia DL, Zhou X, Tao Q (2017) Effect and mechanism of inhibition of PI3K/Akt/mTOR signal pathway on chronic neuropathic pain and spinal microglia in a rat model of chronic constriction injury. Oncotarget 8(32):52923-52934. doi: 10.18632/oncotarget.17629 PMID: 28881783

Objective: To explore the effects of inhibition of PI3K/Akt/mTOR signal pathway on chronic neuropathic pain (CNP) and spinal microglia in a rat model of chronic constriction injury (CCI).

Summary: Inhibition of PI3K/Akt/mTOR signal pathway may alleviate CNP and reduce microglia and GFAP and NGF expressions in marrow in a rat model of CCI.

Usage: immunofluorescence assay (1:500)

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Kulkarni S, Micci MA, Leser J, Shin C, Tang SC, Fu YY, Liu L, Li Q, Saha M, Li C, Enikolopov G, Becker L, Rakhilin N, Anderson M, Shen X, Dong X, Butte MJ, Song H, Southard-Smith EM, Kapur RP, Bogunovic M, Pasricha PJ (2017) Adult enteric nervous system in health is maintained by a dynamic balance between neuronal apoptosis and neurogenesis. Proc Natl Acad Sci U S A 114(18):E3709-E3718. doi: 10.1073/pnas.1619406114 PMID: 28420791

Objective: To identify adult enteric neural precursor cell (ENPC) that would enable a new understanding of the pathogenesis of enteric neuromuscular diseases as well as the development of novel regenerative therapies.

Summary: The results show significant turnover and neurogenesis of adult enteric neurons and provide a paradigm for understanding the enteric nervous system in health and disease.

Usage: IHC (1:400)

Related Products: NGFR (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)