References

Napoli M, Wu SJ, Gore BL, Abbas HA, Lee K, Checker R, Dhar S, Rajapakshe K, Tan AC, Lee MG, Coarfa C, Flores ER (2022) ΔNp63 regulates a common landscape of enhancer associated genes in non-small cell lung cancer. Nat Commun 13(1):614. doi: 10.1038/s41467-022-28202-1 PMID: 35105868

Objective: To investigate the underlying mechanistic role regulated by ΔNp63 in lung cancer development.

Summary: Use of a ΔNp63-specific conditional knockout mouse model and xenograft models of lung adenocarcinoma (LUAD) and squamous cell carcinoma (LUSC). Results show that ΔNp63 promotes non-small cell lung cancer by maintaining the lung stem cells necessary for lung cancer cell initiation and progression in quiescence. ΔNp63 regulates enhancers of cell identity genes.

Usage: Immunofluorescence and IHC (1:100)

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Barros JFF, Sant’Ana AMS, Dias LC, Murashima AAB, Silva LECMD, Fantucci MZ, Rocha EM (2022) Comparison of the effects of corneal and lacrimal gland denervation on the lacrimal functional unit of rats. Arq Bras Oftalmol 85(1):59-67. doi: 10.5935/0004-2749.20220008

Summary: This study compared the changes in the lacrimal functional unit in the following two models of neurogenic dry eye syndrome: sensory denervation of the cornea versus autonomic denervation of the lacrimal gland.

Usage: Rats received 2.5 μg of 192-IgG-SAP (Cat. #IT-01) directly into the left extraorbital lacrimal gland.

Related Products: 192-IgG-SAP (Cat. #IT-01)

See Also:

• Aicher S et al. Denervation of the Lacrimal Gland Leads to Corneal Hypoalgesia in a Novel Rat Model of Aqueous Dry Eye Disease. Invest Ophthalmol Vis Sci 56:6981-6989, 2015.

Hickerson BT, Daniels-Wells TR, Payes C, Clark LE, Candelaria PV, Bailey KW, Sefing EJ, Zink S, Ziegenbein J, Abraham J, Helguera G, Penichet ML, Gowen BB (2022) Host receptor-targeted therapeutic approach to counter pathogenic New World mammarenavirus infections. Nat Commun 13(1):558. doi: 10.1038/s41467-021-27949-3 PMID: 35091550

Objective: Demonstrate that a fusion protein of the antibody (ch128.1/IgG1) directed against the apical domain of human transferrin receptor 1 (hTfR1) and the Machupo virus (MACV) can inhibit infection of attenuated and pathogenic New World mammarenaviruses (NWMs).

Summary: NWMs cause life-threatening hemorrhagic fever (HF) and these viruses enter into cells via hTfR1. Use of ch128.1/IgG1 with other promising direct-acting small molecule antivirals or antibodies targeting the viral envelope glycoprotein would provide a complementary therapeutic strategy that would increase efficacy and reduce the emergence of drug resistance.

Usage: References MonoBiotin-ZAP reacted with avidinylated anti-hTfR (ch128.1Av) in a 1:1 molar ratio on ice for 30 minutes.

Related Products: MonoBiotin-ZAP (Cat. #BT-ZAP)

See Also:

• Daniels-Wells TR et al. Insights into the mechanism of cell death induced by saporin delivered into cancer cells by an antibody fusion protein targeting the transferrin receptor 1. Toxicol In Vitro 27(1):220-231, 2013.
• Daniels TR et al. Conjugation of an anti transferrin receptor IgG3-avidin fusion protein with biotinylated saporin results in significant enhancement of its cytotoxicity against malignant hematopoietic cells. Mol Cancer Ther 6:2995-3008, 2007.

Polito L (2022) Antibody based delivery of toxins and other active molecules for cancer therapy. Biomedicines 10(2):267. doi: 10.3390/biomedicines10020267 PMID: 35203476

Summary: This introduction to studies collected in a special issue that confirmed the potential of immunotherapy for targeted therapy in different cancer models. In the near future, antibody-based therapeutic approaches could improve the outcomes of cancer patients, overcoming some difficulties associated with standard therapy.

Biomedicines, Volume 10, Issue 2 (February 2022) – 318 articles

Pinelli F, Saadati M, Zare EN, Makvandi P, Masi M, Sacchetti A, Rossi F (2023) A perspective on the applications of functionalized nanogels: promises and challenges. International Materials Reviews 68(1):1-25. doi: 10.1080/09506608.2022.2026864

Summary: A perspective on the applications of functionalized nanogels: their features such as large surface area, ability to hold molecules, flexibility in size and water-based formulations have ensured them great recognition as drug delivery systems with various in vivo applications which have confirmed their potential.

Konsman JP (2022) Cytokines in the Brain and Neuroinflammation: We Didn’t Starve the Fire!. Pharmaceuticals (Basel) 15(2):140. doi: 10.3390/ph15020140 PMID: 35215252

Summary: To test the role of IL-1 receptor brain macrophages, the work of Chaskiel et al. employed IL-1 coupled to the ribosome toxin, saporin, and administered this conjugate into forebrain ventricles to effectively reduce the number of CD163-positive perivascular macrophages with no reduction of food intake after subsequent IL-1beta administration.

See Also:

• Chaskiel L et al. Interleukin-1 reduces food intake and body weight in rat by acting in the arcuate hypothalamus. Brain Behav Immun 81:560-573, 2019.

Hankerd K (2021) Female-specific mechanisms of nociplastic pain in murine model. The University of Texas Medical Branch at Galveston, Dept Neuroscience Thesis.

Objective: To study nociplastic pain the authors developed a murine model in which postinjury thermal stimulation of injured area triggers the transition to a nociplastic pain state more readily in females.

Summary: Postinjury stimulation of an injured area triggers the transition from transient pain to nociplastic pain, females are more susceptible to this transition, and allyl isothiocyanate -sensitive afferents at the previously injured area maintain the nociplastic pain state in a female gonadal hormone-dependent manner.

Usage: Intrathecal injection of Mac-1-SAP (IT-06) 8.85 mM in mice.

Related Products: Mac-1-SAP mouse/human (Cat. #IT-06)

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Borkowski LF, Keilholz AN, Smith CL, Canda KA, Nichols NL (2021) Nonsteroidal anti-inflammatory drug (ketoprofen) delivery differentially impacts phrenic long-term facilitation in rats with motor neuron death induced by intrapleural CTB-SAP injections. Exp Neurol 347:113892. doi: 10.1016/j.expneurol.2021.113892

Objective: To determine the effect of ketoprofen delivery on enhanced phrenic long-term facilitation (pLTF)

Summary: pLTF was surprisingly attenuated in 7d CTB-SAP rats and enhanced in 28d CTB-SAP rats (both p < 0.05) following ketoprofen delivery.

Usage: Rats received bilateral intrapleural injections of CTB-SAP; 25 μg dissolved in PBS.

Related Products: CTB-SAP (Cat. #IT-14)

Schulze J, Staecker H, Wedekind D, Lenarz T, Warnecke A (2022) Expression pattern of brain-derived neurotrophic factor and its associated receptors: Implications for exogenous neurotrophin application. Hear Res 413:108098. doi: 10.1016/j.heares.2020.108098 PMID: 33143996

Objective: To investigate the mechanisms of brain-derived neurotrophic factor (BDNF) in the inner ear, by analyzing the expression of mature BDNF (mBDNF), its proform proBDNF and their respective receptors the low affinity p75 neurotrophin receptor (p75NTR) and the neurotrophic receptor tyrosine kinase 2 (NTRK2).

Summary: Treatment with proBDNF is not toxic for spiral ganglion neuron (SGN) survival but simultaneously not protective. However, combined treatment of mBDNF and proBDNF maintained and perhaps slightly increased the protective effect of mBDNF.

Usage: Immunohistochemistry (1:250); AB-N01AP: NGFr (mu p75) Rabbit Polyclonal

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Joshi R, Batie MR, Fan Q, Varisco BM (2022) Mouse lung organoid responses to reduced, increased, and cyclic stretch. Am J Physiol Lung Cell Mol Physiol 322(1):L162-L173. doi: 10.1152/ajplung.00310.2020 PMID: 34851724

Objective: To test two mouse lung organoid mechanotransductive models to address deficiencies in cell culture models.

Summary: Cyclic stretch increased TGF-β and integrin-mediated signaling, with upstream analysis indicating roles for histone deacetylases, microRNAs, and long noncoding RNAs.

Usage: Immunostaining

Related Products: NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)