References

Leung LS, Petropoulos S, Shen B, Luo T, Herrick I, Rajakumar N, Ma J (2011) Lesion of cholinergic neurons in nucleus basalis enhances response to general anesthetics. Exp Neurol 228(2):259-269. doi: 10.1016/j.expneurol.2011.01.019

Summary: Consciousness and response to general anesthesia have been linked to acetylcholine in the brain. The authors treated rats with 150-ng bilateral injections of 192-IgG-SAP (Cat. #IT-01) into the nucleus basalis magnocellularis to examine this connection. Lesioned animals were more affected by propofol and phenobarbitol than control animals. Some effects of halothane were also increased. The data indicate a role for acetylcholine in the brain in the response to general anesthesia.

Related Products: 192-IgG-SAP (Cat. #IT-01)

Montandon G, Qin W, Liu H, Ren J, Greer JJ, Horner RL (2011) PreBotzinger complex neurokinin-1 receptor-expressing neurons mediate opioid-induced respiratory depression. J Neurosci 31(4):1292-1301. doi: 10.1523/JNEUROSCI.4611-10.2011 PMID: 21273414

Summary: In order to identify the neurons involved with respiratory depression due to administration of opioids, some neuro- transmission networks in the preBotzinger complex were locally manipulated. Among various techniques used to analyze the results was immunohistochemistry with anti-NK1r (Cat. #AB-N04). Results show the preBotzinger complex is responsible for suppression of respiratory rate due to opioids.

Related Products: Antibody to NK-1 Receptor (Cat. #AB-N04)

Ohta S, Imaizumi Y, Okada Y, Akamatsu W, Kuwahara R, Ohyama M, Amagai M, Matsuzaki Y, Yamanaka S, Okano H, Kawakami Y (2011) Generation of human melanocytes from induced pluripotent stem cells. PLoS One 6(1):e16182. doi: 10.1371/journal.pone.0016182 PMID: 21249204

Summary: For immunocytochemistry, cells were fixed with PBS containing 4% PFA for 20 min at room temperature. Then, cells were subjected to immunofluorescence staining (1∶100).

Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)

Wagle M, Mathur P, Guo S (2011) Corticotropin-releasing factor critical for zebrafish camouflage behavior is regulated by light and sensitive to ethanol. J Neurosci 31(1):214-224. doi: 10.1523/JNEUROSCI.3339-10.2011 PMID: 21209207

Objective: To explore neural circuit assembly and function using the hardwired camouflage response of zebrafish.

Summary: Both light exposure and ethanol affect the camouflage response. Understanding this system could provide a tool to further investigate the effect of alcohol on neural circuits.

Usage: Immunostaining (1:500)

Related Products: Corticotropin Releasing Hormone Rabbit Polyclonal (Cat. #AB-02)

Goehler LE, Gaykema RPA (2011) Featured Article: Targeted lesion of caudal brainstem catecholamine neurons reveals their role in symptoms of fatigue. Targeting Trends 12(1)

Related Products: Anti-DBH-SAP (Cat. #IT-03), Mouse IgG-SAP (Cat. #IT-18)

Read the featured article in Targeting Trends.

Zhang L, Hadley GA (2010) Application of anti-CD103 immunotoxin for saving islet allograft in context of transplantation. Chin Med J (Engl) 123(24):3644-51.

Summary: This work investigates whether depletion of CD103-positive cells protects transplanted islets from host-immune cell attack. Diabetes was induced in mice, followed by an islet transplant. Anti-CD103-SAP (Cat. #IT-50) was administered via i.p. injection (1.0 mg/kg or 2.0 mg/kg). Rat IgG-SAP (Cat. #IT-17) was used as a control. Diabetic mice treated with anti-CD103-SAP after islet transplantation had an indefinite survival time as compared to untreated mice that survived fewer than 20 days.

Related Products: Anti-CD103-SAP (Cat. #IT-50), Rat IgG-SAP (Cat. #IT-17)

Cerminara NL (2010) Cerebellar modules: individual or composite entities?. J Neurosci 30(48):16065-16067. doi: 10.1523/JNEUROSCI.4823-10.2010

Summary: This short review discusses the compartmentalization of cerebellar modules. Much research has been done to associate particular motor control functions with particular modules. Chemical lesioning is an inadequate technique because the lesion is non-specific. The use of CTB-SAP (Cat. #IT-14) to affect the function of a single module is discussed.

Related Products: CTB-SAP (Cat. #IT-14)

Yang MY, Chaudhary A, Seaman S, Dunty J, Stevens J, Elzarrad MK, Frankel AE, St Croix B (2011) The cell surface structure of tumor endothelial marker 8 (TEM8) is regulated by the actin cytoskeleton. Biochim Biophys Acta 1813(1):39-49. doi: 10.1016/j.bbamcr.2010.11.013

Summary: Tumor endothelial marker 8 (TEM8) is a cell surface protein that is up-regulated on tumor blood vessels. Overexpression of this protein, however, produces a form that is not recognized by the SB5 monoclonal antibody used to bind TEM8. While cells expressing normal levels of TEM8 were killed by an application of biotinylated SB5 plus either 1 nM or 10 nM streptavidin-ZAP (Cat. #IT-27), cells overexpressing the protein did not bind the immunotoxin. Understanding the structural differences between the two forms of TEM8 will help in the design of therapeutic antibodies against these tumor cells.

Related Products: Streptavidin-ZAP (Cat. #IT-27)

Marshall RS, D’Avila F, Di Cola A, Traini R, Spano L, Fabbrini MS, Ceriotti A (2011) Signal peptide-regulated toxicity of a plant ribosome-inactivating protein during cell stress. Plant J 65(2):218-29. doi: 10.1111/j.1365-313X.2010.04413.x PMID: 21223387

Summary: Type I ribosome inactivating proteins (RIPs) are thought to have a role in defending plants against viral or fungal infections. Most type I RIPs have signal peptides for insertion into the endoplasmic reticulum, followed by transportation to a vacuole or the cell wall. The authors examined signal peptide regulation under stress in tobacco plants transfected with saporin. One method of analysis was western blots using anti-saporin (Cat. #AB-15).

Related Products: Saporin Goat Polyclonal (Cat. #AB-15)

Joseph EK, Levine JD (2010) Mu and delta opioid receptors on nociceptors attenuate mechanical hyperalgesia in rat. Neuroscience 171(1):344-350. doi: 10.1016/j.neuroscience.2010.08.035

Summary: In this work the authors analyzed nociceptor populations mediating mechanical hyperalgesia in the rat. Rats received 3.2 µg of IB4-SAP (Cat. #IT-10) into the subarachnoid space between the L4 and L5 vertebrae. Hyperalgesia due to the administration of NGF was inhibited by DAMGO and SNC even in lesioned animals. These data indicate that most nociceptor populations are involved in mechanical hyperalgesia, and that the mu opioid and delta opioid receptors are co-expressed on some TrkA-positive nociceptors.

Related Products: IB4-SAP (Cat. #IT-10)