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Immune tolerance induction using the thyrotropin receptor epitope 78-94 (p37) prevents Graves’ disease in HLA-DR3 transgenic mice
Inaba H, Nonaka I, Hashimoto D, Hirono M, Morita S, Kimura H, Iwakura H, Akamizu T, Nakata M (2025) Immune tolerance induction using the thyrotropin receptor epitope 78-94 (p37) prevents Graves’ disease in HLA-DR3 transgenic mice. Front Immunol 16:1633350. doi: 10.3389/fimmu.2025.1633350 PMID: 41256857
Objective: To investigate whether pre-immunization with the immunodominant TSH-R–derived peptide spanning residues 78–94 (ISRIYVSIDVTLQQLES; p37) could induceimmune tolerance and prevent Graves’ disease in DR3 transgenic mice.
Summary: GD was induced by intramuscular injection of adenovirus encoding human TSH-R (Ad-TSH-R289). Mice were pretreated with p37 either as a single 50 mg dose or by step-up escalation protocol (0.05 mg, 0.5 mg, and 5 mg), with or without a final 50 mg dose. The authors findings demonstrate that step-up immunization with p37 induces antigen-specific immune tolerance and effectively prevents the development of GD in HLA-DR3 transgenic mice.
Usage: Expression of TSH-R (1-289) was confirmed by Western blot (AB-N16)
Related Products: TSH Receptor (A10) Mouse Monoclonal (Cat. #AB-N16)
Human embryonic stem cells form functional thyroid follicles
Ma R, Latif R, Davies TF (2015) Human embryonic stem cells form functional thyroid follicles. Thyroid 25(4):455-461. doi: 10.1089/thy.2014.0537 PMID: 25585054
Summary: Lysates were prepared from single- and double-transfected cells by treating with 1 ·RIPA buffer for 20 min on ice. The samples were then centrifuged and treated with 5 · sample buffer at 100°C for 5 min and loaded on a 4–15% SDS-PAGE gel. Western blotting was performed according to a standard protocol.
Related Products: TSH Receptor (A10) Mouse Monoclonal (Cat. #AB-N16)
Direct binding of thyrotropin receptor autoantibody to in vitro translated thyrotropin receptor: A comparison to radioreceptor assay and thyroid stimulating bioassay.
Morgenthaler NG, Hodak K, Seissler J, Steinbrenner H, Pampel I, Gupta M, McGregor AM, Scherbaum WA, Banga JP. (1999) Direct binding of thyrotropin receptor autoantibody to in vitro translated thyrotropin receptor: A comparison to radioreceptor assay and thyroid stimulating bioassay. Thyroid 9(5):467-475. PMID: 10365678
Related Products: TSH Receptor (A7) Mouse Monoclonal (Cat. #AB-N14), TSH Receptor (A9) Mouse Monoclonal (Cat. #AB-N15), TSH Receptor (A10) Mouse Monoclonal (Cat. #AB-N16)
Characterisation of the antibody response to the extracellular region of recombinant thyrotropin receptor.
Morgenthaler NG, Kim MR, Gardas A, Vlase H, Aust G, Gupta M, McGregor AM, Banga JP (1997) Characterisation of the antibody response to the extracellular region of recombinant thyrotropin receptor. Autoimmunity 26(2):75-84. doi: 10.3109/08916939709003850 PMID: 9546816
Related Products: TSH Receptor (A10) Mouse Monoclonal (Cat. #AB-N16)
Monoclonal antibodies to the human TSH receptor: epitope mapping and binding to the native receptor on the basolateral plasma membrane of the thyroid follicular cells.
Nicholson LB, Vlase H, Graves P, Nilsson M, Molne J, Huang GC, Morgenthaler NG, Davies TF, McGregor AM, Banga JP (1996) Monoclonal antibodies to the human TSH receptor: epitope mapping and binding to the native receptor on the basolateral plasma membrane of the thyroid follicular cells. J Mol Endocrinol 16:159-170. doi: 10.1677/jme.0.0160159 PMID: 9156519
Related Products: TSH Receptor (A7) Mouse Monoclonal (Cat. #AB-N14), TSH Receptor (A9) Mouse Monoclonal (Cat. #AB-N15), TSH Receptor (A10) Mouse Monoclonal (Cat. #AB-N16)