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Isolation of blood-brain barrier-crossing antibodies from a phage display library by competitive elution and their ability to penetrate the central nervous system
Thom G, Hatcher J, Hearn A, Paterson J, Rodrigo N, Beljean A, Gurrell I, Webster C (2018) Isolation of blood-brain barrier-crossing antibodies from a phage display library by competitive elution and their ability to penetrate the central nervous system. mAbs 10:304-314. doi: 10.1080/19420862.2017.1409320 PMID: 29182455
Summary: Fluorescence microvolume assay technology (FMAT) periprep extract or Nickel-purified scFvs from E. coli were transferred into assay plates containing 10 ml (1:1000 dilution of a 1 mg/ml stock solution) mouse anti-HIS MAb.)
Related Products: 6His Mouse Monoclonal (Cat. #AB-213)
Brain penetration, target engagement, and disposition of the blood-brain barrier-crossing bispecific antibody antagonist of metabotropic glutamate receptor type 1.
Webster C, Caram-Salas N, Haqqani A, Thom G, Brown L, Rennie K, Yogi A, Costain W, Brunette E, Stanimirovic D (2016) Brain penetration, target engagement, and disposition of the blood-brain barrier-crossing bispecific antibody antagonist of metabotropic glutamate receptor type 1. FASEB J 30:1927-1940. doi: 10.1096/fj.201500078 PMID: 26839377
Summary: To generate a BBB-transmigrating antibody that could be reformatted to full IgG, scientists started with the BBB-crossing llama single domain antibody FC5. Standard phage display protocols were used to isolate single-chain variable fragments (scFv) from the FC5-scFv library. 6His Mouse Monoclonal antibody (Cat. #AB-213) was used to assess cell binding of scFvs of FC5 using fluorescence microvolume assay technology. An scFv that competed with FC5 binding was selected for further testing. An antibody antagonist of the metabotropic glutamate receptor-1 was fused with this scFv antibody fragment (BBB-mGluR1) and tested in an in vitro BBB model. The resulting bispecific antibody retained selective mGluR1 binding and saw a 20-fold enhanced rate of transcytosis across the BBB compared to fusion with control antibody fragment. Intravenous injection of BBB-mGluR1 had analgesic properties in a rat model of persistent inflammatory pain.
Related Products: 6His Mouse Monoclonal (Cat. #AB-213)
Epitopes of the highly immunogenic trichomonas vaginalis alpha-actinin are serodiagnostic targets for both women and men.
Neace CJ, Alderete JF (2013) Epitopes of the highly immunogenic trichomonas vaginalis alpha-actinin are serodiagnostic targets for both women and men. J Clin Microbiol 51(8):2483-2490. doi: 10.1128/JCM.00582-13 PMID: 23616456
Summary: Trichomonas vaginalis is an anaerobic protozoan that is the most common nonviral causative agent for sexually-transmitted infections. The presence of T. vaginalis in men is usually asymptomatic, making it difficult to assess exposure to the organism. The authors examined sera from exposed individuals for reactivity to specific epitopes of trichomonad α-actinin. A recombinant version of trichonomad α-actinin was constructed and detected using Anti-6His (Cat. #AB-213). Some epitopes were reactive with sera from both men and women, making them potential diagnostic targets.
Related Products: 6His Mouse Monoclonal (Cat. #AB-213)