May Z, Kumar R, Fuehrmann T, Tam R, Vulic K, Forero J, Lucas Osma A, Fenrich K, Assinck P, Lee M, Moulson A, Shoichet M, Tetzlaff W, Biernaskie J, Fouad K (2018) Adult skin-derived precursor Schwann cell grafts form growths in the injured spinal cord of Fischer rats. Biomed Mater 13:034101. doi: 10.1088/1748-605X/aa95f8 PMID: 29068322

Objective: To improve grafted cell survival in the injured spinal cord, which is typically low.

Summary: It is of utmost importance to define the precise culture/transplantation parameters for maintenance of normal cell function and safe and effective use of cell therapy.

Usage: Immunohistochemistry (1:500)

Related Products: NGFr (192-IgG, p75) Mouse Monoclonal (Cat. #AB-N43)

Ovsepian SV, Antyborzec I, O’Leary VB, Zaborszky L, Herms J, Oliver Dolly J (2013) Neurotrophin receptor p75 mediates the uptake of the amyloid beta (Abeta) peptide, guiding it to lysosomes for degradation in basal forebrain cholinergic neurons. Brain Struct Funct 219(5):1527-1541. doi: 10.1007/s00429-013-0583-x PMID: 23716278

Summary: Accumulation of β-amyloid in the brain is considered one of the main causes of Alzheimer’s disease. The increase in β-amyloid is accompanied by a reduction in levels of the high affinity nerve growth factor receptor (trkA) and cognitive impairment. The authors looked at levels of the low affinity nerve growth factor receptor (p75) that do not decline. Using a 0.8-μg injection of 192-Cy3 (Cat. #FL-01) into the medial prefrontal cortex of rats the authors assessed the transport of p75 and β-amyloid by microscopy. The results indicate that the primary destinations of both p75 and β-amyloid were the late endosome and lysosome.

Related Products: 192-IgG Mouse Monoclonal, Cy3-labeled (Cat. #AB-N43FL3)

Ovsepian SV, Dolly JO, Zaborszky L (2012) Intrinsic voltage dynamics govern the diversity of spontaneous firing profiles in basal forebrain noncholinergic neurons. J Neurophysiol 108(2):406-418. doi: 10.1152/jn.00642.2011 PMID: 22496531

Summary: The voltage modulation functions of the basal forebrain have commonly been associated with cholinergic cells. More recent work has suggested that noncholinergic cells have an influence on this type of neuronal activity. The authors labeled cholinergic neurons by injecting 0.8-1.6 μg of Cy3-192-IgG (Cat. #FL-01) into the lateral ventricles of rats. Patch-clamp recordings were taken from brain slices of these animals under various blockade conditions. The results demonstrate that neuropeptide Y receptors as well as ions such as Ca2+ and K+ are important for regenerative firing in basal forebrain cholinergic neurons.

Related Products: 192-IgG Mouse Monoclonal, Cy3-labeled (Cat. #AB-N43FL3)

Iwatsuki K, Yoshimine T, Kishima H, Aoki M, Yoshimura K, Ishihara M, Ohnishi Y, Lima C (2008) Transplantation of olfactory mucosa following spinal cord injury promotes recovery in rats. Neuroreport 19(13):1249-1252. doi: 10.1097/WNR.0b013e328305b70b PMID: 18695502

Related Products: NGFr (192-IgG, p75) Mouse Monoclonal (Cat. #AB-N43)

Huh CY, Danik M, Manseau F, Trudeau LE, Williams S (2008) Chronic exposure to nerve growth factor increases acetylcholine and glutamate release from cholinergic neurons of the rat medial septum and diagonal band of Broca via mechanisms mediated by p75NTR. J Neurosci 28(6):1404-1409. doi: 10.1523/JNEUROSCI.4851-07.2008 PMID: 18256260

Related Products: 192-IgG Mouse Monoclonal, Cy3-labeled (Cat. #AB-N43FL3)

Chin JH, Ma L, MacTavish D, Jhamandas JH (2007) Amyloid beta protein modulates glutamate-mediated neurotransmission in the rat basal forebrain: involvement of presynaptic neuronal nicotinic acetylcholine and metabotropic glutamate receptors. J Neurosci 27:9262-9269. doi: 10.1523/JNEUROSCI.1843-07.2007 PMID: 17728440

Summary: This work focused on the effect of amyloid beta on glutamate-mediated neurotransmission in the diagonal band of Broca. Using neurons identified by staining with Cy3-labeled 192-IgG (Cat. #FL-01, 5 µl of 1:1 diluted antibody injected into the left and right ventricle) the authors monitored the response to amyloid beta by measuring excitatory postsynaptic currents via whole-cell patch-clamp recordings. The results suggest that glutamate neurotransmission might be vulnerable to Alzheimer’s disease, and may also be a therapeutic target.

Related Products: 192-IgG Mouse Monoclonal, Cy3-labeled (Cat. #AB-N43FL3)

Momiyama T, Fukazawa Y (2007) D1-like dopamine receptors selectively block P/Q-type calcium channels to reduce glutamate release onto cholinergic basal forebrain neurones of immature rats. J Physiol 580(Pt 1):103-117. doi: 10.1113/jphysiol.2006.125724 PMID: 17234695

Related Products: 192-IgG Mouse Monoclonal, Cy3-labeled (Cat. #AB-N43FL3)

Momiyama T, Zaborszky L (2006) Somatostatin presynaptically inhibits both GABA and glutamate release onto rat basal forebrain cholinergic neurons. J Neurophysiol 96(2):686-694. doi: 10.1152/jn.00507.2005 PMID: 16571735

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Xu C, Michelsen KA, Wu M, Morozova E, Panula P, Alreja M (2004) Histamine innervation and activation of septohippocampal GABAergic neurones: Involvement of local ACh release. J Physiol 561(Pt 3):657-670. doi: 10.1113/jphysiol.2004.071712 PMID: 15486020

Related Products: 192-IgG Mouse Monoclonal, Cy3-labeled (Cat. #AB-N43FL3)

Wu M, Newton SS, Atkins JB, Xu C, Duman RS, Alreja M (2003) Acetylcholinesterase inhibitors activate septohippocampal GABAergic neurons via muscarinic but not nicotinic receptors. J Pharmacol Exp Ther 307(2):535-543. doi: 10.1124/jpet.103.052514 PMID: 12966162

Related Products: 192-IgG Mouse Monoclonal, Cy3-labeled (Cat. #AB-N43FL3)