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Staining with FITC-labeled Anti-Saporin

Q: I plan to use your Secondary Antibody Conjugates, Rab-ZAP (Cat. #IT-05), and Fab-ZAP Rabbit (Cat. #IT-57) with my primary antibody and would like to observe eliminated cells using a fluorescence microscope. The idea is to co-culture cancer cells and fibroblast cells, and kill fibroblast cells only with a specific primary antibody. Then I want to observe the eliminated fibroblast cells and take pictures with a fluorescence microscope. Can you recommend a protocol?

A: In order to stain and visualize the cells that are being eliminated, it would be best to stain for Saporin using a fluorescently-tagged antibody such as the FITC-labeled Saporin antibody (Cat. #FL-02). By washing off the media after a day, and then staining for saporin, one would illuminate only the cells that have internalized the saporin (marking them for death). The cells that do not stain for saporin will live.

Related Product: FITC-labeled Saporin antibody (Cat. #FL-02)

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