pHast Conjugate Options

Our line of pHast conjugates are one of our fastest tools to quantitatively test your primary antibody’s specificity, binding, and internalization, providing results in 1 day.
The pHast conjugate uses a secondary antibody that is cross-linked to a pH-dependent fluorescent reporter and will piggy-back onto your primary antibody.
This fluorescent reporter will increase intensity as the pH of its surroundings becomes more acidic, such as you would see on the inside of a cell.
There are various options available in our pHast Conjugates line.
Our pHast conjugates can recognize and bind to various species, such as human, mouse, rat, rabbit, goat, guinea pig, chicken, and the newest arrivals are the conjugates that can bind to VHH domain, typically seen in heavy chain in alpaca IgG.
We also offer streptavidin conjugated to our pHast dyes which opens up the choice to use a biotinylated targeting agent.
Not only do we offer various species for targeting, but we also offer various pH dependent fluorescent dyes, allowing you to multiplex in the same experiment.
- Red dye (Ex: 532 nm/Em: 560 nm),
- Far-red dye(Ex: 643 nm/Em: 660 nm),
- Green dye (Ex: 453 nm/Em: 522 nm)
- Blue dye(Ex: 362 nm/Em: 452 nm).