Cytomegalovirus (CMV) belongs to the Betaherpesvirinae subfamily of Herpesviridae which includes herpes simplex virus types 1 and 2, varicella-zoster virus, and Epstein-Barr virus. The herpes viruses share a characteristic ability to remain latent over long periods. CMV is a double-stranded linear DNA virus with 162 hexagonal protein capsomeres surrounded by a lipid membrane. CMV has the largest genome of the herpes viruses, ranging from 230-240 kilobase pairs. Human CMV is composed of unique and inverted repeats that include the existence of 4 genome isomers caused by inversion of L-S genome components (class E). Replication may be divided into immediate early, delayed early, and late gene expression based on time of synthesis after infection. The DNA is replicated by rolling circles. In vitro, CMV replicates in human fibroblasts.
The E. coli derived recombinant artificial mosaic protein contains the cytomegalovirus (CMV) gB immunodominant regions (amino acids 11-67), fused with a 26 kDa GST tag. The molecular weight of the gB region protein is 6.5 kDa; the total molecular weight of the recombinant protein is 32.5 kDa. It is purified by a proprietary chromatographic technique. Purity is >95% as determined by 10% SDS-PAGE and coomassie staining. The protein is immunoreactive with sera from CMV-infected individuals.
Reported to be effective for ELISA and immunoblotting (western blot) and is an excellent antigen for detection of CMV with minimal specificity problems.
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