FITC-labeled donkey anti-goat IgG can be used to verify specific binding of goat IgG to its receptor. By first incubating cells with the primary goat antibody, and then binding the FITC-labeled donkey anti-goat IgG to the primary antibody, a fluorescent marker is formed that can demonstrate expression of a receptor or affinity of an antibody for its receptor. FITC is excited by 488 nm wavelength light, and emits at 525 nm.
This fluorescent conjugate was prepared using donkey anti-goat IgG (H+L) and the fluorescent compound, fluorescein isothiocyanate (FITC). The antibody binds to goat IgG, and is affinity-purified to decrease background and non-specific binding. This antibody exhibits maximal binding to goat IgG antibodies, and minimal cross-reactivity with other molecules. This product is routinely tested by flow cytometry.
Applications include flow cytometry.
To view protocol(s) for this and other products please visit: www.ATSbio.com/support/protocols