FITC-labeled goat anti-rabbit IgG can be used to verify specific binding of rabbit IgG to its receptor. By first incubating cells with the primary rabbit antibody, and then binding the FITC-labeled goat anti-rabbit IgG to the primary antibody, a fluorescent marker is formed that can demonstrate expression of a receptor or affinity of an antibody for its receptor. FITC is excited by 488 nm wavelength light, and emits at 525 nm.
This fluorescent conjugate was prepared using goat anti-rabbit IgG (H+L) and the fluorescent compound, fluorescein isothiocyanate (FITC). The antibody binds to rabbit IgG, and is affinity-purified to decrease background and non-specific binding. This antibody exhibits maximal binding to rabbit IgG antibodies, and minimal cross-reactivity with other molecules.
Applications include flow cytometry.
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