Monoclonal antibody was obtained after BALB/c mouse immunization with the conjugate: Histamine-Hexamethylene diisocyanate-Carrier proteins and hybridization of spleen cells with the myeloma cell line SP2/O/Ag14. Ascites production was performed in BALB/c mice. The ascitic fluid was purified by ammonium sulfate precipitation.
This antibody targets conjugated histamine. This antibody does not recognize free histamine.
Using a conjugate Histamine-(Pc), antibody specificity was performed with an ELISA test by competition experiments with the following compounds: Histamine-HD-(Pc) (1), L-Methyl-Histamine-HD-(Pc) (1), Histamine-G-(Pc) (1/>50,000), Histamine-GA-(Pc) (1/>50,000), Histidine-HD-(Pc) (1/>50,000).
Applications include ELISA and immunoblotting.
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