Monoclonal antibody was obtained after BALB/c mouse immunization with the conjugates: Dopamine-Carrier proteins and hybridization of spleen cells with the myeloma cell line SP2/O/Ag14. Ascites production was performed in BALB/c mice. The ascites fluid was purified by ammonium sulfate precipitation.
This antibody targets conjugated dopamine. This antibody does not recognize free dopamine.
Using a conjugate Dopamine-Glutaraldehyde-BSA, antibody specificity was performed with an ELISA test by competition experiments with the following compounds: Dopamine-G-BSA (1), L-DOPA-G-(Pc) (1/10,000), Tyrosine-G-(Pc) (1/36,000), Tyramine-G-(Pc) (1/>50,000), Noradrenaline-G-(Pc) (1/>50,000), Octopamine-G-(Pc) (1/>50,000), Adrenaline-G-(Pc) (1/>50,000), Dopamine (1/>50,000).
Applications include ELISA, immunocytochemistry, immunohistochemistry, immunoblotting.
To view protocol(s) for this and other products please visit: www.ATSbio.com/support/protocols