Antiserum previously preabsorbed on protein carriers and purified by ammonium sulfate precipitation.
This antibody targets conjugated GABA (Gamma-Aminobutyric acid). This antibody does not recognize free GABA.
Using a conjugate GABA-Glutaraldehyde-BSA, antibody specificity was performed with an ELISA test by competition experiments with the following compounds: GABA-G-BSA (1), ÌÙ-Alanine-G-(Pc) (1/10,000), Glycine-G-(Pc) (1/20,000), D-Aspartate-G-(Pc) (1/>50,000), D-Glutamate-G-(Pc) (1/>10,000).
Tested applications include immunocytochemistry and immunohistochemistry
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