Antiserum previously preabsorbed on protein carriers and purified by ammonium sulfate precipitation.
This antibody targets conjugated dopamine. This antibody does not recognize free dopamine.
Using a conjugate Dopamine-Glutaraldehyde-BSA, antibody specificity was performed with an ELISA test by competition experiments with the following compounds: Dopamine-G-BSA (1), Noradrenaline-G-(Pc) (1/200), L-DOPA-G-(Pc) (1/400), Octopamine-G-(Pc) (1/400), Tyramine-G-(Pc) (1/>5,000), Adrenaline-G-(Pc) (1/>5,000), Dopamine (1/>5,000).
Tested applications include immunocytochemistry and ELISA.
To view protocol(s) for this and other products please visit: www.ATSbio.com/support/protocols