Antiserum previously preabsorbed on protein carriers and purified by ammonium sulfate precipitation.
This antibody targets conjugated L-dihydroxyphenylalanine (L-DOPA). This antibody does not recognize free L-DOPA.
Using a conjugate L-DOPA-Glutaraldehyde-BSA, antibody specificity was performed with an ELISA test by competition experiments with the following compounds: L-DOPA-G-BSA (1), alpha-methyl-L-DOPA-G-BSA (1/2,200), 3-O-mehtyl-L-DOPA-G-BSA (1/50,000), Dopamine-G-BSA (1/>50,000), Noradrenaline-G-BSA (1/>50,000), Tyrosine-G-BSA (1/>50,000).
Applications include ELISA, immunocytochemistry, immunohistochemistry, immunoblotting.
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