This antibody targets conjugated Trans-4-Hydroxy-L-Proline. This antibody does not recognize free Trans-Hydroxyproline.
Using a Trans-hydroxyproline absorbed on bovine serum albumin, antibody specificity was performed with an ELISA test by competition experiments with the following compounds: Trans-Hydroxyproline-BSA (1), Cis-hydroxyproline-BSA (1), Proline-BSA (1/40), Pyrroline-5-carboxylic acid-BSA (1/900).
Applications include ELISA, Immunohistochemistry, Western Blot.