Antiserum previously preabsorbed on protein carriers and purified by ammonium sulfate precipitation.
This antibody targets conjugated 5-Hydroxytryptamine. This antibody does not recognize free 5-Hydroxytryptamine.
Using a conjugate 5-hydroxytryptamine-Glutaraldehyde-BSA, antibody specificity was performed with an ELISA test by competition experiments with the following compounds: 5-Hydroxytryptamine-G-BSA (1), 5-Methoxytryptamine-G-(Pc) (1/25), Tryptamine-G-(Pc) (1/50), 5-Hydroxytryptophan-G-(Pc) (1/175), 5-Hydroxytryptamine (1/1,150), Tryptophan-G-(Pc) (1/3,500), 5-Methoxytryptophan-G-(Pc) (1/20,000).
Tested applications include immunocytochemistry and immunohistochemistry.
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