Antiserum previously preabsorbed on protein carriers and purified by ammonium sulfate precipitation.
This antibody targets conjugated choline-glutaric acid. This antibody does not recognize free choline-glutaric acid. Staining with glutaraldehyde fixative creates conjugated acetylcholine in tissue.
Using a conjugate Choline-glutaric acid-BSA antibody specificity was performed with an ELISA test by competition experiments with the following compounds: Choline-GA-BSA (1), Choline-allyl alcohol-G-BSA (1/1.6), Phosphatidylcholine (1/>10,000), Choline (1/>10,000), Acetylcholine (1/>10,000).
Applications include ELISA and immunohistochemistry / immunocytochemistry.
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