6His Mouse Monoclonal [AB-213]


SKU: AB-213 Category: Quantity: 100 ug | Antibody Type: Monoclonal | Host: mouse | Conjugate: unconjugated | Usage: dot blot, ELISA, immunoblotting, immunoprecipitation, immunostaining |

The use of polyhistidine tags has become a popular method for protein purification, commonly used in the screening process as a tag for your protein or peptide of interest. This polyhistidine epitope tag is generally comprised of six consecutive histidine amino acid residues located at the N-terminal, C-terminal, or internally. The 6 His-Tag is widely used because of its affinity to bind nickel or cobalt metal ions attached to sepharose, which can then be used to purify the protein in a native or denatured state.

Recognizes His-tagged recombinant proteins. The concentration is 1 mg/ml.

Reported to be effective for immunoblotting, immunoprecipitation and immunostaining.

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