The use of polyhistidine tags has become a popular method for protein purification, commonly used in the screening process as a tag for your protein or peptide of interest. This polyhistidine epitope tag is generally comprised of six consecutive histidine amino acid residues located at the N-terminal, C-terminal, or internally. The 6 His-Tag is widely used because of its affinity to bind nickel or cobalt metal ions attached to sepharose, which can then be used to purify the protein in a native or denatured state.
Recognizes His-tagged recombinant proteins. The concentration is 1 mg/ml.
Reported to be effective for immunoblotting, immunoprecipitation and immunostaining.
keywords: 6His, 6-his, 6His-tag, 6-His-tag, His-Tag, N-terminal, C-terminal, Anti-6His, Anti-6-His