Calculator: Preparing a Streptavidin-ZAP Conjugate

Biotinylated material + Streptavidin-ZAP are used in an equimolar ratio. View calculations below for the amount of biotinylated material, Streptavidin-ZAP, and PBS will be needed to mix your Streptavidin-ZAP conjugate.

Prepare Tube of Biotinylated Material Reacted with Streptavidin-ZAP:

  • Add the calculated volume of Streptavidin-ZAP plus the calculated volume of Biotinylated Material. 
  • Pipet up and down to mix and incubate at room temperature for 15 minutes.
  • Add PBS to bring up to final volume.
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