Targeting Talk: Neurotransmitter Antibodies

Advanced Targeting Systems offers a full line of antibodies to neurotransmitters; everything from serotonin and L-DOPA, to noradrenaline and dopamine. These antibodies are an excellent choice for immunohistochemistry applications as they are ideally primed for use in tissues that have been perfused with a mixture of paraformaldehyde and gluteraldehyde.

Some frequently asked questions:

Q:  Could you confirm if Anti-Conjugated Caprylic Acid (Cat. #AB-T084) can detect free Caprylic acid or only when conjugated with BSA or another carrier protein?

A:  This antibody does not recognize BSA or need Caprylic acid to be conjugated to BSA in order for it to bind. However, it DOES need to be used in the presence of gluteraldehyde in order to create the proper epitope for the antibody to recognize the Caprylic acid.

Q:  I was wondering whether it could be possible to receive more information about the Gibberellic acid antibody (Cat. #AB-T130)? Is it possible to use this antibody to recognize free gibberellic acid by a direct ELISA system?

A:  The most common use for this product is immunohistochemistry. ELISA is used to test cross-reactivity and is not a recommended application for these antibodies. On its own, gibberellic acid is too small a molecule to provide a complete effective epitope, so for IHC the tissue is perfused with a gluteraldehyde component which provides the epitope complement needed for the antibody to recognize gibberellic acid.

Q:  For your conjugated neurotransmitter antibodies, what concentrations and sizes do you provide?

A:  All of these antibodies are sold in 50-mcl volumes. No concentrations are needed  for these products as they all have recommended dilutions for use listed on the data sheets.

Q:  What protocol should I use for these antibodies?

A:  We recommend using the protocols available on our website. Some have specific protocol recommendations which you can link to from the product page.

Questions? Ask a Product Manager