Q: Your targeted toxin kits come with different controls. I’m not sure of the best way to use them; there is included unconjugated antibody, unconjugated saporin, and a control conjugate, mouse IgG-SAP. Should I use them all in the same experiment or for different purposes?
A: For mouse IgG-containing conjugates, the ideal control is Mouse IgG-SAP (Cat. # IT-18). Mouse IgG-SAP — that is, saporin conjugated to mouse IgG — that has no specific antigen for targeting is the best control. Unconjugated saporin is still considered a second good control, useful in cases where down-regulation by the antibody is a concern.
Q: Which control is best to use with Octreotide-SAP?
A: The best control to use with Octreotide-SAP is Blank-SAP (Cat. #IT-21). Blank-SAP serves as a control for all our peptide-targeted SAP conjugates. Listed below are the appropriate controls to use with our primary saporin conjugates.
|Blank-CTA (IT-61)||peptide-targeted CTA conjugates||SP-CTA and Neurotensin-CTA|
|Blank-SAP (IT-21)||peptide-targeted SAP conjugates||SSP-SAP, MOR-SAP, CRF-SAP, NPY-SAP, CCK-SAP, Galanin- SAP, Bombesin-SAP, Oxytocin- SAP, Neurotensin-SAP, NK3- SAP, Dyno-SAP and NMB-SAP|
|Fab IgG-SAP (IT-67)||goat IgG Fab-ZAP secondary conjugates||Fab-ZAP mouse, Fab-ZAP human, Fab-ZAP rat, Fab-ZAP rabbit and FabFc-ZAP human|
|Goat IgG-SAP (IT-19)||goat IgG-containing targeted toxins||Mab-ZAP, Rab-ZAP, Hum-ZAP, Rat-ZAP, Anti-M-ZAP, Hug-M- ZAP and gPIG-ZAP|
|Human IgG-SAP (IT-49)||human IgG-containing targeted toxins||custom conjugates|
|Mouse IgG-SAP (IT-18)||mouse IgG-containing targeted toxins||192-IgG-SAP, OX7-SAP, Anti- DBH-SAP, ME20.4-SAP, Anti- SERT-SAP, Anti-CD25-SAP human, Mac-1-SAP rat, Anti- CD22-SAP, Anti-6 His-ZAP, Anti- GFP-ZAP, Anti-Basigin2-SAP, Anti-V5-ZAP, and Anti-FLAG (M5)-ZAP|
|Mouse IgM-SAP (IT-41)||mouse IgM-containing targeted toxins||Anti-M-ZAP|
|Rabbit IgG-SAP (IT-35)||rabbit IgG-containing targeted toxins||mu p75-SAP, GAT1-SAP, Goat- ZAP, Anti-ChAT-SAP, Melanopsin-SAP and Chick-ZAP|
|Rat IgG-SAP (IT-17)||rat IgG-containing targeted toxins||Mac-1-SAP, Anti-DAT-SAP, Anti- CD25-SAP mouse and Anti- CD103-SAP|