Targeting Talk: Flow Cytometry Services

Q: Recently we used your flow cytometry services (Cytometry Research, ATS subsidiary). Based on post flow data analysis needs, I am providing the assay group list below (withheld for confidentiality).

A: Yes, all these antibodies meet the requirement of being excited by our 488nm laser, however the specific combinations you list use fluorescent probes that have emission wavelengths that are too similar and would actually be detected on the same fluorescent channel. In essence, you would be unable to differentiate which target was being detected. Here are suggestions for common combinations of commercially available fluorescent probes if you are interested in analyzing three colors simultaneously. All these probes can be excited at 488nm and will work on our equipment, so just make sure you don’t have more than one probe from each channel.

Flow Table