Does AB-T183 (Lauric Acid Rat Polyclonal, Conjugated) recognize lauric acid alone, or does it need to be conjugated to something (a protein carrier)?
This antibody targets conjugated Lauric Acid. It does not recognize free lauric acid. Antisera was preabsorbed on protein carriers and ammonium sulfate-purified. Using a conjugate Lauric acid-Gluteraldehyde-Protein Carrier (PC), antibody specificity was performed with an ELISA test by competition experiments with the following compounds:
| Compounds | Cross-Reactivity Ratios |
| Lauric acid-PC | 1 |
| Caprylic acid-PC | 1/300 |
| Myristic acid-PC | 1/400 |
| Palmitic acid-PC | 1/>50,000 |
| Caproic acid-PC | 1/>50,000 |
| Oleic acid-PC | 1/>50,000 |
Usage: Applications include immunohistochemistry (1/500-1/2,000) and immunocytochemistry.
Controls: Lauric Acid, conjugated, Cat. #AG-183