Targeting Talk: Targeting B Cells

How can I target B-cells? Which secondary conjugate should I use?

ATS makes secondary conjugates that use your primary antibody to target cells for elimination. Just mix your primary antibody with a secondary antibody conjugated to the ribosome-inactivating protein, Saporin, to screen your antibodies. The cells targeted by your primary antibody are eliminated.

Hum-ZAP

Hum-ZAP (Cat. #IT-22) is made with a bivalent secondary antibody that recognizes whole IgG. B-Cells have endogenous IgGs.

whole IgG

Fab-ZAP

Fab-ZAP (Cat. #IT-51) is made with a monovalent secondary antibody that recognizes whole IgG. B-Cells have endogenous IgGs.

 

FabFc-ZAP (Cat. #IT-65) is made with a monovalent secondary antibody that recognizes ONLY the FC portion of IgG.

FC region IgG

FabFc-ZAP

FabFc-ZAP is an excellent choice!