How can I target B-cells? Which secondary conjugate should I use?
ATS makes secondary conjugates that use your primary antibody to target cells for elimination. Just mix your primary antibody with a secondary antibody conjugated to the ribosome-inactivating protein, Saporin, to screen your antibodies. The cells targeted by your primary antibody are eliminated.
Hum-ZAP (Cat. #IT-22) is made with a bivalent secondary antibody that recognizes whole IgG. B-Cells have endogenous IgGs. |
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Fab-ZAP (Cat. #IT-51) is made with a monovalent secondary antibody that recognizes whole IgG. B-Cells have endogenous IgGs. |
FabFc-ZAP (Cat. #IT-65) is made with a monovalent secondary antibody that recognizes ONLY the FC portion of IgG. |
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FabFc-ZAP is an excellent choice! |