Targeting Talk: Product Questions

I was wondering whether it could be possible to receive more information about the Gibberellic acid antibody (Cat. #AB-T130)? Is it possible to use this antibody to recognize free gibberellic acid by a direct ELISA system?

The most common use for this product is immunohistochemistry. The tissue is perfused with a gluteraldehyde component, and that gluteraldehyde provides the epitope complement needed for the antibody to recognize gibberellic acid. On its own, gibberellic acid is too small a molecule to provide a complete effective epitope.

I’d like to know which of your products are the pan-neural targeting toxins? I need an agent to kill all nerves in tissue preps.

OX-7-SAP (Cat. #IT-02) should be perfect for this application. We recommend you examine your neurons with OX-7 antibody to see if they are positive. The only complication would be if you want to look at T-lymphocytes that also express Thy 1.

We are interested in having a custom conjugation of saporin and our antibody. Do you remove unconjugated antibody from the final material you send us?

We do remove the unconjugated antibody and saporin from the final product that we send to you. And perhaps to answer a question you may not be asking, but may be curious about, the unconjugated material is not particularly usable, after it has been removed from the final product as it has been slightly modified in preparation for the conjugation.

We will be using your chick-ZAP secondary conjugate (Cat. #IT-62) and noticed that in your protocol you mention not to use a reducing agent in your media. Our normal growth media contains beta mercaptoethanol at 100 μM. Will this be a problem?

Officially, we would recommend allowing the cells to acclimate to media that contains NO BMe, and then proceed with your experiments. However, some of our in-house experiments use cells that are cultured in media containing 50 μM BMe, and we have not seen that concentration affect the toxin’s effectiveness, but we have not tried a concentration as high as 100 μM.