Targeting Talk: Choosing the Correct Secondary Conjugate

I have a mouse monoclonal antibody and a rabbit polyclonal antibody that I would like to test using your secondary conjugate system. Which products do I need to order?

For mouse monoclonals, you can use: Mab-ZAP (Cat. #IT-04) — Cells that internalize your mouse monoclonal antibody will be eliminated. Or, Fab-ZAP mouse (Cat. #IT-48) — Cells that internalize your mouse monoclonal IgG antibody will be eliminated.
For rabbit polyclonals, you can use: Rab-ZAP (Cat. #IT-05) — Cells that internalize your rabbit polyclonal antibody will be eliminated. Or, Fab-ZAP rabbit (Cat. #IT-57) — Cells that internalize your rabbit IgG antibody will be eliminated.

What is the difference between the Fab-ZAP products and Mab-ZAP or Rab-ZAP?

The difference between Fab-ZAP products and other secondary conjugates is that Fab- ZAP is made with a monovalent secondary antibody which eliminates the possibility of cap formation as cross-linking of the Fab-ZAP molecules cannot occur. The Fab- ZAP products will still recognize the heavy and light chains of antibodies, and should be used in the same way and at the same molar concentrations as the original secondary conjugates (such as Mab-ZAP and Rab-ZAP). Cytotoxicity assays using Fab-ZAP (mouse) have demonstrated an improved EC50 when directly compared to Mab-ZAP.

Any of our secondary conjugates offers a very cost-effective diagnostic method for screening primary antibodies for in vitro or in vivo use.

See also: Secondary Conjugates Catalog