I am interested in your product GAT1-SAP (Cat. #IT-32). Before ordering, I would like to know how long it takes until the toxin produces a lesion; Is seven days enough?
The usual time-course for saporin toxins is that behavioral effects start appearing at four days and usually plateau at seven days. However, keep in mind that it takes some time for dead cells to be cleared out, so histology on the animal should wait until at least two weeks after administration.
After ordering, how long it will take to receive the toxin?
GAT1-SAP is in stock in both the U.S. and our warehouse in The Netherlands. packages are shipped by overnight delivery; Monday-Thursday in the U.S. and Monday-Wednesday in Europe.
Is there any saporin derivative available that selectively destroys all or some hippocampal neurons?
Assuming that you mean to eliminate cell bodies from the hippocampus, rather than just projections, a neat paper (Martin JL, Sloviter RS, J Comp Neurol 436(2):127-152) describes the use of SSP-SAP (Cat #IT-11) to do this. NPY-SAP (Cat. #IT-28) could also be interesting, but there are no publications to demonstrate that.
When we contacted you to find out more about having a custom saporin conjugation performed with our primary antibody, you recommended that we use the ATS secondary conjugate system to determine that our antibody was specific to the population we want to eliminate. We looked more at the website, and it seems that we are supposed to start with Anti-M-ZAP, Cat. #IT-30 (our primary Ab is a mouse IgM), and use the Mouse IgM-SAP, Cat. #IT-41 for control. Is this correct?
We don’t quite understand what the Mouse IgM-SAP control is or how it should be used. If it is a control for the Anti-M-ZAP, then it should consist of saporin coupled to a dummy antibody, and we should add it to our primary in our test. Is it also supposed to be used as a control for a directly conjugated primary+saporin?
If your primary antibody is a mouse IgM, then you are correct that Anti-M-ZAP (Cat# IT-30) is the appropriate secondary conjugate to use. As for control conjugates, the best control would be a secondary conjugate using an IgM isotype control mixed with Anti-M-ZAP. An alternative would be to use Goat IgG-SAP (Cat# IT-19) made with normal goat IgG and mimics Anti-M-ZAp without the specific affinity for mouse IgM.
Once you determine you need a direct conjugate made between your mouse IgM primary antibody and saporin, then you would want to use the Mouse IgM-SAP (Cat# IT-41) as a control toxin just as you use your direct conjugate. ATS currently offers a 50% discount on the purchase of control conjugates when you have a custom saporin conjugation service with your primary targeting agent.