Targeting Trends Newsletter 06q4

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Newsletter Highlights

  • ATS Hits the Field (page 2)
  • Teaser Winners (page 2)
  • Anti-DBH-SAP (page 5)
  • Anti-6-His and Anti-SAP-HRP (page 7)

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Basomedial Hypothalamic Injections of Neuropeptide Y Conjugated to Saporin Selectively Disrupt Hypothalamic Controls of Food Intake – read article
(continued on page 6)

This article is a summary of data presented in the reference below. Figures 1-4 are taken from that article. This work was funded by NS045520 and DK40498 to S. Ritter.
Bugarith K, Dinh TT, Li AJ, Speth RC, Ritter S. (2005) Endocrinology 146:1179-1191.

Product information related to cover article: NPY-SAP (Cat. #IT-28), Anti-DBH-SAP (Cat. #IT-03), Saporin (Cat. #PR-01), Blank-SAP (Cat. #IT-21)

Targeting Teaser Winners

Upcoming Events

  • Society for Neuroscience, Atlanta GA — October 10-14, Booth #1240
  • American Society for Cell Biology, San Diego CA — December 10-13, Booth #734

ATS Hits the Field

Recent Scientific References – read online

Targeting Talk: Anti-DBH-SAP Administration

  • We injected anti-DBH-SAP into the hypothalamus of Sprague-Dawley rats and sacrificed them 2 weeks later. We did not see any reduction in the DBH fiber staining.
    When the drug arrived, we aliquoted it in 1-µl snap-cap tubes on ice, and stored them at -80°C. For injections, a 1-µl aliquot was diluted to alittle over 10 µl so that we had a final concentration of 1 µg/10 µl.We administered two injections of 100 nl on each side (with 10 ng of anti-DBH-SAP) using a 0.5-µl Hamilton syringe attached to a stereotax. The needle was a 33-gauge with a blunt tip. I tried previously to use glass micropipette tips attached to a Hamilton syringe with the line filled with mineral oil, but found that the actual volume displacement was too unreliable.

NPY- SAP Selectively Disrupts Hypothalamic Controls of Food Intake – read article
(continued from page 1)

Targeting Tools

Targeting Technology Tutorial

Targeting Teaser (Jumble)

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