Targeting Trends Newsletter 06q4

Below are links to view the quarterly newsletter Targeting Trends. If you would like to be added to the mailing list, please complete the information on our contact page.

Newsletter Highlights

  • ATS Hits the Field (page 2)
  • Teaser Winners (page 2)
  • Anti-DBH-SAP (page 5)
  • Anti-6-His and Anti-SAP-HRP (page 7)

Basomedial Hypothalamic Injections of Neuropeptide Y Conjugated to Saporin Selectively Disrupt Hypothalamic Controls of Food Intake – read article

This article is a summary of data presented in the reference below. Figures 1-4 are taken from that article. This work was funded by NS045520 and DK40498 to S. Ritter. Bugarith K, Dinh TT, Li AJ, Speth RC, Ritter S. (2005) Endocrinology 146:1179-1191.

Product information related to cover article: NPY-SAP (Cat. #IT-28), Anti-DBH-SAP (Cat. #IT-03), Saporin (Cat. #PR-01), Blank-SAP (Cat. #IT-21)

Targeting Teaser Winners

Upcoming Events

  • Society for Neuroscience, Atlanta GA — October 10-14, Booth #1240
  • American Society for Cell Biology, San Diego CA — December 10-13, Booth #734

ATS Hits the Field

Recent Scientific References – read online

Targeting Talk: Anti-DBH-SAP Administration

  • We injected anti-DBH-SAP into the hypothalamus of Sprague-Dawley rats and sacrificed them 2 weeks later. We did not see any reduction in the DBH fiber staining. When the drug arrived, we aliquoted it in 1-µl snap-cap tubes on ice, and stored them at -80°C. For injections, a 1-µl aliquot was diluted to alittle over 10 µl so that we had a final concentration of 1 µg/10 µl.We administered two injections of 100 nl on each side (with 10 ng of anti-DBH-SAP) using a 0.5-µl Hamilton syringe attached to a stereotax. The needle was a 33-gauge with a blunt tip. I tried previously to use glass micropipette tips attached to a Hamilton syringe with the line filled with mineral oil, but found that the actual volume displacement was too unreliable.

Targeting Tools

Targeting Technology Tutorial

Targeting Teaser (Jumble)