What is a second immunotoxin?
ATS’s second immunotoxins are conjugations of a secondary antibody (as of December 2000, either goat anti-mouse IgG or goat anti-rabbit IgG) to the ribosome-inactivating protein, saporin.
How does a second immunotoxin target?
The second immunotoxin uses the secondary antibody to “piggyback” onto your primary antibody in order to evaluate the ability of the primary antibody to internalize.
What happens when the second immunotoxin gets inside the cell?
If the second immunotoxin is internalized, saporin will inactivate the ribosomes of the cell, thereby causing cell death.
Are there different types of second immunotoxins available?
What is the ratio of antibody to second immunotoxin for in vitro testing?
Both Mab-ZAP and Rab-ZAP have been shown effective in concentrations ranging from 0.5 to 2 moles of primary antibody per mole of second immunotoxin.
See also: Second Immunotoxins Catalog