Cy3-labeled 192-IgG Mouse Monoclonal References

192-IgG Mouse Monoclonal, Cy3-labeled (Cat. #FL-01)

14 entries found for : fl-01

Neurotrophin receptor p75 mediates the uptake of the amyloid beta (Abeta) peptide, guiding it to lysosomes for degradation in basal forebrain cholinergic neurons.

Ovsepian SV, Antyborzec I, O’Leary VB, Zaborszky L, Herms J, Oliver Dolly J (2013) Neurotrophin receptor p75 mediates the uptake of the amyloid beta (Abeta) peptide, guiding it to lysosomes for degradation in basal forebrain cholinergic neurons. Brain Struct Funct 219(5):1527-1541. doi: 10.1007/s00429-013-0583-x

Summary: Accumulation of β-amyloid in the brain is considered one of the main causes of Alzheimer's disease. The increase in β-amyloid is accompanied by a reduction in levels of the high affinity nerve growth factor receptor (trkA) and cognitive impairment. The authors looked at levels of the low affinity nerve growth factor receptor (p75) that do not decline. Using a 0.8-μg injection of 192-Cy3 (Cat. #FL-01) into the medial prefrontal cortex of rats the authors assessed the transport of p75 and β-amyloid by microscopy. The results indicate that the primary destinations of both p75 and β-amyloid were the late endosome and lysosome.

Related Products: 192-IgG Mouse Monoclonal, Cy3-labeled (Cat. #FL-01)

Intrinsic voltage dynamics govern the diversity of spontaneous firing profiles in basal forebrain noncholinergic neurons.

Ovsepian SV, Dolly JO, Zaborszky L (2012) Intrinsic voltage dynamics govern the diversity of spontaneous firing profiles in basal forebrain noncholinergic neurons. J Neurophysiol 108(2):406-418. doi: 10.1152/jn.00642.2011

Summary: The voltage modulation functions of the basal forebrain have commonly been associated with cholinergic cells. More recent work has suggested that noncholinergic cells have an influence on this type of neuronal activity. The authors labeled cholinergic neurons by injecting 0.8-1.6 μg of Cy3-192-IgG (Cat. #FL-01) into the lateral ventricles of rats. Patch-clamp recordings were taken from brain slices of these animals under various blockade conditions. The results demonstrate that neuropeptide Y receptors as well as ions such as Ca2+ and K+ are important for regenerative firing in basal forebrain cholinergic neurons.

Related Products: 192-IgG Mouse Monoclonal, Cy3-labeled (Cat. #FL-01)

Chronic exposure to nerve growth factor increases acetylcholine and glutamate release from cholinergic neurons of the rat medial septum and diagonal band of Broca via mechanisms mediated by p75NTR.

Huh CY, Danik M, Manseau F, Trudeau LE, Williams S (2008) Chronic exposure to nerve growth factor increases acetylcholine and glutamate release from cholinergic neurons of the rat medial septum and diagonal band of Broca via mechanisms mediated by p75NTR. J Neurosci 28(6):1404-1409. doi: 10.1523/JNEUROSCI.4851-07.2008

Related Products: 192-IgG Mouse Monoclonal, Cy3-labeled (Cat. #FL-01)

Amyloid beta protein modulates glutamate-mediated neurotransmission in the rat basal forebrain: involvement of presynaptic neuronal nicotinic acetylcholine and metabotropic glutamate receptors.

Chin JH, Ma L, MacTavish D, Jhamandas JH (2007) Amyloid beta protein modulates glutamate-mediated neurotransmission in the rat basal forebrain: involvement of presynaptic neuronal nicotinic acetylcholine and metabotropic glutamate receptors. J Neurosci 27:9262-9269. doi: 10.1523/JNEUROSCI.1843-07.2007

Summary: This work focused on the effect of amyloid beta on glutamate-mediated neurotransmission in the diagonal band of Broca. Using neurons identified by staining with Cy3-labeled 192-IgG (Cat. #FL-01, 5 µl of 1:1 diluted antibody injected into the left and right ventricle) the authors monitored the response to amyloid beta by measuring excitatory postsynaptic currents via whole-cell patch-clamp recordings. The results suggest that glutamate neurotransmission might be vulnerable to Alzheimer’s disease, and may also be a therapeutic target.

Related Products: 192-IgG Mouse Monoclonal, Cy3-labeled (Cat. #FL-01)

D1-like dopamine receptors selectively block P/Q-type calcium channels to reduce glutamate release onto cholinergic basal forebrain neurones of immature rats.

Momiyama T, Fukazawa Y (2007) D1-like dopamine receptors selectively block P/Q-type calcium channels to reduce glutamate release onto cholinergic basal forebrain neurones of immature rats. J Physiol 580(Pt 1):103-117. doi: 10.1113/jphysiol.2006.125724

Related Products: 192-IgG Mouse Monoclonal, Cy3-labeled (Cat. #FL-01)

Somatostatin presynaptically inhibits both GABA and glutamate release onto rat basal forebrain cholinergic neurons.

Momiyama T, Zaborszky L (2006) Somatostatin presynaptically inhibits both GABA and glutamate release onto rat basal forebrain cholinergic neurons. J Neurophysiol 96(2):686-694. doi: 10.1152/jn.00507.2005

Related Products: 192-IgG Mouse Monoclonal, Cy3-labeled (Cat. #FL-01)

Histamine innervation and activation of septohippocampal GABAergic neurones: Involvement of local ACh release.

Xu C, Michelsen KA, Wu M, Morozova E, Panula P, Alreja M (2004) Histamine innervation and activation of septohippocampal GABAergic neurones: Involvement of local ACh release. J Physiol 561(Pt 3):657-670. doi: 10.1113/jphysiol.2004.071712

Related Products: 192-IgG Mouse Monoclonal, Cy3-labeled (Cat. #FL-01)

Acetylcholinesterase inhibitors activate septohippocampal GABAergic neurons via muscarinic but not nicotinic receptors.

Wu M, Newton SS, Atkins JB, Xu C, Duman RS, Alreja M (2003) Acetylcholinesterase inhibitors activate septohippocampal GABAergic neurons via muscarinic but not nicotinic receptors. J Pharmacol Exp Ther 307(2):535-543. doi: 10.1124/jpet.103.052514

Related Products: 192-IgG Mouse Monoclonal, Cy3-labeled (Cat. #FL-01)

Physiological and pharmacological characteristics of the inhibitory muscarinic response in septohippocampal cholinergic neurons.

Wu M, Xu C, Alreja M (2002) Physiological and pharmacological characteristics of the inhibitory muscarinic response in septohippocampal cholinergic neurons. Neuroscience 2002 Abstracts 35.7. Society for Neuroscience, Orlando, FL.

Summary: Septohippocampal cholinergic neurons in the MSDB provide the hippocampus with almost its entire ACh and also release ACh locally within the MSDB. The released ACh sustains activity in the GABAergic limb of the septohippocampal pathway. Septohippocampal cholinergic neurons undergo atrophy in neurodegenerative disorders associated with loss of cognition. In a recent study we demonstrated that 65% of septohippocampal cholinergic neurons are inhibited by ACh via muscarinic receptors. Because of the importance of ACh and septohippocampal cholinergic neurons in cognition, we studied the physiological and pharmacological properties of the muscarinic response in MSDB neurons. Using intracellular and whole-cell recordings, we tested the effects of muscarine on retrogradely-labeled septohippocampal cholinergic neurons in vitro in rat brain slices. The cells were labeled using the Cy3-192IgG, a selective marker of septohippocampal cholinergic neurons. Prolonged (10-15 mins) but not short (1-2 min) applications of muscarine or oxotremorine produced a marked desensitization (>50%). The muscarine-induced outward current was found to be mediated via direct as well as indirect mechanisms. It reversed at Ek and was blocked by external barium. The M2/M4 antagonist, methoctramine blocked the muscarine response in only 10% of the neurons tested and tropicamide, an M4-prefering antagonist, blocked the muscarine response in 5/5 neurons tested, suggesting possible involvement of M4 receptors.

Related Products: 192-IgG Mouse Monoclonal, Cy3-labeled (Cat. #FL-01)

Immunolesioning of identified motoneuron pools following intramuscular injection of the immunotoxin, 192-IgG-saporin, in neonatal rats.

Peterson WE, Jordan LM, Brownstone RM (2001) Immunolesioning of identified motoneuron pools following intramuscular injection of the immunotoxin, 192-IgG-saporin, in neonatal rats. Neuroscience 2001 Abstracts 626.14. Society for Neuroscience, San Diego, CA.

Summary: Studies have shown that the immunotoxin, 192-IgG-Saporin, can selectively lesion p75-positive cholinergic neurons of the basal forebrain in adult rats. Here we demonstrate the novel use of 192-IgG-Saporin to induce MN loss following intramuscular (I.M.) injection in neonatal rats. Two days following I.M. injection of 192-IgG-Cy3, neonatal rats (but not adult rats or neonatal mice) had Cy3-labeled MNs. This suggests that the 192-IgG antibody and its conjugates can be internalised by receptor-mediated endocytosis and retrogradely transported to spinal motor neurons. To induce MN loss, the left hind limb musculature of anaesthetised Sprague-Dawley rats were exposed, and several muscles injected with 0.5μg of 192-IgG-Saporin (Chemicon). Right hind-limb muscles were injected with DiI. Animals were sacrificed 25 days later. Ten μm coronal sections were obtained using a cryostat and Nissl stained. The neonatal rats showed signs of a locomotor deficit 2.5 weeks post injection with 192-IgG-Saporin, which increased slightly in severity over the next week and a half. Nissl stained coronal sections of the lumbar region showed an obvious MN deficit on the 192-IgG-Saporin treated side compared to control side. The injected muscles were also severely atrophic, a not unexpected finding given that they too express p75 receptors. We conclude that 192-IgG-Saporin can be used to lesion MN pools when IM injected in neonatal rats. This model may prove useful for testing cell replacement therapies for the treatment of MN diseases like amyotrophic lateral sclerosis (ALS).

Related Products: 192-IgG Mouse Monoclonal, Cy3-labeled (Cat. #FL-01)

Septal innervation of the hippocampus regulates expression α7 nicotinic receptors in CA1 and CA3 pyramidal neurons.

Camara AL, Pereira EF, Alkondon M, Randall WR, Castro NG, Cintra WM, Albuquerque EX (2001) Septal innervation of the hippocampus regulates expression α7 nicotinic receptors in CA1 and CA3 pyramidal neurons. Neuroscience 2001 Abstracts 145.1. Society for Neuroscience, San Diego, CA.

Summary: To investigate the effects of septal innervation on expression of α7 nicotinic receptors (nAChRs) in CA1 and CA3 pyramidal neurons in the hippocampus, the patch-clamp technique and confocal microscopy were applied to organotypic hippocampal cultures and septal-hippocampal co-cultures. In the co-cultures, septal fibers labeled with DiI were visualized in the hippocampus. Field stimulation of septal fibers also resulted in postsynaptic currents that could be recorded from CA1 and CA3 pyramidal neurons in the hippocampus. These currents had glutamatergic, GABAergic and cholinergic components. The latter originated most likely from the septal cholinergic neurons that were labeled in situ with the cholinergic marker Cy3-192 IgG. α7 nAChRs in the somatodendritic region of CA1 and CA3 pyramidal neurons in the hippocampus in cultures and co-cultures were activated by the α7 nAChR agonist choline, which elicited type IA currents, and were visualized by labeling with rhodamine-conjugated α-bungarotoxin (Rho-α-BGT). After 21 days in vitro, the amplitude of type IA currents was substantially smaller in pyramidal neurons in septal-hippocampal co-cultures than in hippocampal oragnotypic cultures. Labeling of the somatodendritic region of hippocampal pyramidal neurons with Rho-α-BGT was also less intense in the organotypic co-cultures than in cultures. These results suggest that functional septal innervation of the hippocampus regulates the expression of α7 nAChRs in hippocampal pyramidal neurons.

Related Products: 192-IgG Mouse Monoclonal, Cy3-labeled (Cat. #FL-01)

Muscarinic tone sustains impulse flow in the septohippocampal GABA but not cholinergic pathway: implications for learning and memory.

Alreja M, Wu M, Liu W, Atkins JB, Leranth C, Shanabrough M (2000) Muscarinic tone sustains impulse flow in the septohippocampal GABA but not cholinergic pathway: implications for learning and memory. J Neurosci 20(21):8103-8110. doi: 10.1523/JNEUROSCI.20-21-08103.2000

Related Products: 192-IgG Mouse Monoclonal, Cy3-labeled (Cat. #FL-01)

Cholinergic excitation of septohippocampal GABA but not cholinergic neurons: implications for learning and memory.

Wu M, Shanabrough M, Leranth C, Alreja M (2000) Cholinergic excitation of septohippocampal GABA but not cholinergic neurons: implications for learning and memory. J Neurosci 20(10):3900-3908. doi: 10.1523/JNEUROSCI.20-10-03900.2000

Summary: It has long been assumed that the drug-induced enhancement of learning and memory in both young and aged rats was accomplished through a cholinergic pathway in the hippocampus. Wu et al. used a fluorescent labeling molecule, 192-IgG conjugated to Cy3 (Custom Service from ATS) to visualize these neurons. They found that the effects of cognition-enhancing drugs are not facilitated through action on cholinergic neurons. Instead, activation of GABA neurons is implicated in this model.

Related Products: 192-IgG Mouse Monoclonal, Cy3-labeled (Cat. #FL-01)

Selective in vivo fluorescence labelling of cholinergic neurons containing p75(NTR) in the rat basal forebrain.

Hartig W, Seeger J, Naumann T, Brauer K, Bruckner G. (1998) Selective in vivo fluorescence labelling of cholinergic neurons containing p75(NTR) in the rat basal forebrain. Brain Res 808(2):155-165. doi: 10.1016/s0006-8993(98)00792-6

Related Products: 192-IgG Mouse Monoclonal, Cy3-labeled (Cat. #FL-01)