Angiotensin II receptor References

Angiotensin II receptor (AT-1AR) Rabbit Polyclonal, affinity-purified (Cat. #AB-N25AP),
Angiotensin II receptor (AT-1BR) Rabbit Polyclonal, affinity-purified (Cat. #AB-N26AP),
Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP),
Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

65 entries found for : angiir

Bovine bone gelatin-derived peptides: Food processing characteristics and evaluation of antihypertensive and antihyperlipidemic activities

Cao S, Wang Z, Xing L, Zhou L, Zhang W (2022) Bovine bone gelatin-derived peptides: Food processing characteristics and evaluation of antihypertensive and antihyperlipidemic activities. J Agric Food Chem 70(32):9877-9887. doi: 10.1021/acs.jafc.2c02982

Objective: To evaluate the food processing properties of bovine bone gelatin-derived peptides (BGPs) and their effects and mechanisms on hypertension and hypertension complications in spontaneously hypertensive rats.

Summary: BGPs could alleviate hypertension and dyslipidemia in SHRs by inhibiting ACE/Ang II/AT1R and activating the Ang II/AT2R signaling pathway.

Usage: Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified

Related Products: Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

Spike-mediated ACE2 down-regulation was involved in the pathogenesis of SARS-CoV-2 infection

Gao X, Zhang S, Gou J, Wen Y, Fan L, Zhou J, Zhou G, Xu G, Zhang Z (2022) Spike-mediated ACE2 down-regulation was involved in the pathogenesis of SARS-CoV-2 infection. J Infect 85(4):418-427. doi: 10.1016/j.jinf.2022.06.030

Objective: To determine the role of angiotensin converting enzyme 2 (ACE2) as the receptor for SARS-CoV-2 entry, is also an important regulator of renin-angiotensin system (RAS) homeostasis, which plays an unsettled role in the pathogenesis of COVID-19.

Summary: The downregulation of ACE2 potentially links COVID-19 to the imbalance of RAS.

Usage: Western blot

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Angiotensin II enhances group 2 innate lymphoid cell responses via AT1a during airway inflammation.

Liu G, Chen Y, Wang Y, Deng X, Xiao Q, Zhang L, Xu H, Han X, Lei A, He J, Li X, Cao Y, Zhou P, He C, Wu P, Jiang W, Tan M, Chen C, Yang Q, Lu L, Deng K, Yao Z, Zhou J (2022) Angiotensin II enhances group 2 innate lymphoid cell responses via AT1a during airway inflammation. J Exp Med 219(3):e20211001. doi: 10.1084/jem.20211001

Objective: To identify angiotensin (Ang) II as a positive regulator of Group 2 innate lymphoid cells (ILC2s).

Summary: Ang II plays a critical role in regulating ILC2 responses and airways inflammation.

Usage: Administration of Ang II (30ug) i.n. or i.p. daily for 5 days.

Related Products: Angiotensin II receptor (AT-1AR) Rabbit Polyclonal, affinity-purified (Cat. #AB-N25AP)

Local renin angiotensin system and sperm DNA fragmentation

Aparicio Prieto MV, Rodríguez Gallego MV, Valdivia Palacín A, Franco Iriarte Y, Hervás Barbara G, Echevarría Orella E, Casis Saenz L (2022) Local renin angiotensin system and sperm DNA fragmentation. Asian J Androl 24(2):139-146. doi: 10.4103/aja202150

Objective: The renin angiotensin system (RAS) appears to influence male fertility at multiple levels. The relationship between the RAS and DNA integrity was analyzed.

Summary: Fragmented DNA spermatozoa have a lower capacity to respond to bioactive RAS peptides.

Usage: Immunocytochemistry

Related Products: Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

Aqueous extract of Solanum nigrum attenuates Angiotensin-II induced cardiac hypertrophy and improves cardiac function by repressing protein kinase C-ζ to restore HSF2 deSUMOlyation and Mel-18-IGF-IIR signaling suppression

Lin HJ, Mahendran R, Huang HY, Chiu PL, Chang YM, Day CH, Chen RJ, Padma VV, Liang-Yo Y, Kuo WW, Huang CY (2021) Aqueous extract of Solanum nigrum attenuates Angiotensin-II induced cardiac hypertrophy and improves cardiac function by repressing protein kinase C-ζ to restore HSF2 deSUMOlyation and Mel-18-IGF-IIR signaling suppression. J Ethnopharmacol 8:114728. doi: 10.1016/j.jep.2021.114728

Objective: To investigate the ability of Solanum nigrum to attenuate Angiotensin II - induced cardiac hypertrophy and improve cardiac function through the suppression of protein kinase PKC-ζ and Mel-18-IGF-IIR signaling leading to the restoration of HSF2 desumolyation.

Summary: The data reveals the cardioprotective effect of S. nigrum inhibiting PKC-ζ with alleviated IGF IIR level in the heart that profoundly remits cardiac hypertrophy for hypertension-induced heart failure.

Usage: Western Blot

Related Products: Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

Hypertensive effects of transforming growth factor-β1 in vascular smooth muscles cells from spontaneously hypertensive rats are mediated by sulfatase 2

Kim HS, Kim HY (2022) Hypertensive effects of transforming growth factor-β1 in vascular smooth muscles cells from spontaneously hypertensive rats are mediated by sulfatase 2. Cytokine 150:155754. doi: 10.1016/j.cyto.2021.155754

Objective: To examine the role of extracellular sulfatases on TGF-β1-induced effects associated with the expression of mediators related to hypertension in vascular smooth muscle cells (VSMCs) from spontaneously hypertensive rats (SHR).

Summary: The hypertensive effects of TGF-β1 through the AT1 R pathway were mainly dependent on Sulf2 activity in SHR VSMCs. Taken together, these results suggest that Sulf2, but not Sulf1, plays a major role in mediating the increased effects of TGF-β1 in hypertensive VSMCs.

Usage: Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified

Related Products: Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

The protective effects of AT2R agonist, CGP42112A, against angiotensin ii-induced oxidative stress and inflammatory response in astrocytes: role of AT2R/PP2A/NFκB/ROS signaling

Bhat SA, Fatima Z, Sood A, Shukla R, Hanif K (2021) The protective effects of AT2R agonist, CGP42112A, against angiotensin ii-induced oxidative stress and inflammatory response in astrocytes: role of AT2R/PP2A/NFκB/ROS signaling. Neurotox Res 39(6):1991-2006. doi: 10.1007/s12640-021-00403-4

Objective: To evaluate the role and molecular mechanism of AT2R agonist CGP against Angiotensin II-induced astrocytic activation in primary astrocytes, and in a rat model of hypertension.

Summary: AT2R activation by CGP abrogated Ang II-induced astrocytic activation, by mitigating the ROS production, mitochondrial dysfunction, IκB-α degradation, NFκB nuclear translocation, and release of TNF-α in astrocytes.

Related Products: Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

Tetrahydrocurcumin ameliorates kidney injury and high systolic blood pressure in high-fat diet-induced type 2 diabetic mice

Sangartit W, Ha KB, Lee ES, Kim HM, Kukongviriyapan U, Lee EY, Chung CH (2021) Tetrahydrocurcumin ameliorates kidney injury and high systolic blood pressure in high-fat diet-induced type 2 diabetic mice. Endocrinol Metab (Seoul) 36(4):810-822. doi: 10.3803/EnM.2021.988

Objective: To investigate the protective effect of tetrahydrocurcumin (THU) on intrarenal RAS expression, kidney injury, and systolic blood pressure (SBP) in high-fat diet (HFD)-induced type 2 diabetic mice.

Summary: THU alleviated kidney injury in mice with HFD-induced type 2 diabetes, possibly by blunting the activation of the intrarenal RAS/nicotinamide adenine dinucleotide phosphate oxidase IV (NOX4)/monocyte chemoattractant protein 1 (MCP-1) axis and by lowering the high SBP.

Usage: Western Blot (1:1000)

Related Products: Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

Baoyuan decoction (BYD) attenuates cardiac hypertrophy through ANKRD1-ERK/GATA4 pathway in heart failure after acute myocardial infarction

Meng H, Du Z, Lu W, Wang Q, Sun X, Jiang Y, Wang Y, Li C, Tu P (2021) Baoyuan decoction (BYD) attenuates cardiac hypertrophy through ANKRD1-ERK/GATA4 pathway in heart failure after acute myocardial infarction. Phytomedicine 89:153617. doi: 10.1016/j.phymed.2021.153617

Objective: To explore whether BYD plays a protective role against heart failure (HF) by attenuating cardiac hypertrophy (CH) via the ANKRD1-ERK/GATA4 pathway.

Summary: BYD ameliorates CH and improves HF through the ANKRD1-ERK/GATA4 pathway, providing a novel therapeutic option for the treatment of HF.

Usage: Western Blot

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Angiotensin (1-7) alleviates postresuscitation myocardial dysfunction by suppressing oxidative stress through the phosphoinositide 3-kinase, protein kinase b, and endothelial nitric oxide synthase signaling pathway

Zhu L, Liu Z, Huang LP, Zhou HR, Cao Y, Yang XP, Wang BJ, Yang ZL, Chen J (2021) Angiotensin (1-7) alleviates postresuscitation myocardial dysfunction by suppressing oxidative stress through the phosphoinositide 3-kinase, protein kinase b, and endothelial nitric oxide synthase signaling pathway. J Cardiovasc Pharmacol 78(1):e65-e76. doi: 10.1097/fjc.0000000000001037

Objective: To investigate the role of the Ang (1-7)-MasR axis in postresuscitation myocardial dysfunction (PRMD) and its associated mechanism.

Summary: The Ang (1-7)-MasR axis can alleviate PRMD by reducing myocardial tissue damage and oxidative stress through activation of the phosphoinositide 3-kinase-protein kinase B-endothelial nitric oxide synthase signaling pathway and provide a new direction for the clinical treatment of PRMD.

Usage: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Suppression of inflammatory cardiac cytokine network in rats with untreated obesity and pre-diabetes by AT2 receptor agonist NP-6A4

Gavini MP, Mahmood A, Belenchia AM, Beauparlant P, Kumar SA, Ardhanari S, DeMarco VG, Pulakat L (2021) Suppression of inflammatory cardiac cytokine network in rats with untreated obesity and pre-diabetes by AT2 receptor agonist NP-6A4. Front Pharmacol 12:693167. doi: 10.3389/fphar.2021.693167

Objective: To determine whether NP-6A4 (a new AT2R peptide agonist with an FDA orphan drug designation for pediatric cardiomyopathy) would mitigate cardiac damage from chronic inflammation induced by untreated obesity.

Summary: Seventeen pro-inflammatory and pro-fibrotic cytokines that increase during lethal cytokine storms caused by infections such as COVID-19 were among the cytokines suppressed by NP-6A4 treatment in ZO rat heart. Thus, NP-6A4 activates a novel anti-inflammatory network comprised of 21 proteins in the heart that was not reported previously. Since NP-6A4’s unique mode of action suppresses pro-inflammatory cytokine network and attenuates myocardial damage, it can be an ideal adjuvant drug with other anti-glycemic, anti-hypertensive, standard-of-care drugs to protect the heart tissues from pro-inflammatory and pro-fibrotic cytokine attack induced by obesity.

Usage: Immunohistochemistry, 5-micrometer heart sections

Related Products: Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

Implication of RAS in postnatal cardiac remodeling, fibrosis and dysfunction induced by fetal undernutrition

Rodríguez-Rodríguez P, Vieira-Rocha MS, Quintana-Villamandos B, Monedero-Cobeta I, Prachaney P, López de Pablo AL, González MDC, Morato M, Diniz C, Arribas SM (2021) Implication of RAS in postnatal cardiac remodeling, fibrosis and dysfunction induced by fetal undernutrition. Pathophysiology 28(2):273-290. doi: 10.3390/pathophysiology28020018

Objective: To assess if alterations in renal artery stenosis (RAS) during lactation participate in cardiac dysfunction associated with fetal undernutrition.

Summary: In rats exposed to fetal undernutrition, RAS disbalance and associated cardiac remodeling during lactation may set the basis for later heart dysfunction.

Usage: Immunohistochemistry (1:50)

Related Products: Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

Cardiac pathology in Mucopolysaccharidosis I mice: Losartan modifies ERK1/2 activation during cardiac remodeling.

Gonzalez EA, Leitão SAT, Dos Santos Soares D, Tavares AMV, Giugliani R, Baldo G, Matte U (2021) Cardiac pathology in Mucopolysaccharidosis I mice: Losartan modifies ERK1/2 activation during cardiac remodeling. J Inherit Metab Dis 44(3):740-750. doi: 10.1002/jimd.12327

Objective: To investigate whether the pathways influenced by losartan and propranolol may modulate the cardiac remodeling process in MPS I mice.

Summary: Losartan and propranolol restore the heart function and could be used to ameliorate the cardiac disease in MPS I.

Usage: Western Blot 1:400

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Mechanism of Radix Astragali and Radix Salviae Miltiorrhizae ameliorates hypertensive renal damage

Hou G, Jiang Y, Zheng Y, Zhao M, Chen Y, Ren Y, Wang C, Li W (2021) Mechanism of Radix Astragali and Radix Salviae Miltiorrhizae ameliorates hypertensive renal damage. Biomed Res Int eCollection:5598351. doi: 10.1155/2021/5598351

Objective: To explore the mechanism of combination of Radix Astragali (RA) and Radix Salviae Miltiorrhizae (RS) ameliorating HRD by regulation of the renal sympathetic nerve.

Summary: The combination of RA and RS may inhibit the hyperexcitability of sympathetic nerves, reduce Ang II overexpression, avoid abnormal activation of the RAS system, and improve rat renal blood flow resistance, accordingly maintaining the normal physiological structure and function of kidney tissue and having a protective effect on the cardiovascular system.

Usage: Immunohistochemistry (1:150)

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Vehicle emissions-exposure alters expression of systemic and tissue-specific components of the renin-angiotensin system and promotes outcomes associated with cardiovascular disease and obesity in wild-type C57BL/6 male mice

Phipps BL, Suwannasual U, Lucero J, Mitchell NA, Lund AK (2021) Vehicle emissions-exposure alters expression of systemic and tissue-specific components of the renin-angiotensin system and promotes outcomes associated with cardiovascular disease and obesity in wild-type C57BL/6 male mice. Toxicol Rep 8:846-862. doi: 10.1016/j.toxrep.2021.04.001

Objective: To investigate the hypothesis that exposure to engine emissions increases systemic and local adipocyte RAS signaling, promoting the expression of factors involved in cardiovascular disease and obesity.

Summary: Subchronic traffic-generated air pollution exposure (MVE) results in elevated expression of AT1 in the systemic vasculature, which is associated with increased vascular adhesion molecule expression, monocyte/macrophage sequestration, and production of ROS, all of which are early tissue-level signaling events in the pathogenesis of CVD. Furthermore, kidney levels of renin and Ang II receptors, AT1 and AT2, were also elevated with MVE exposure.

Usage: Immunofluorescence (1:1000)

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP), Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

[Effect of salt intake on residual renal function in rats receiving peritoneal dialysis]

Kang H, Wang Y, Zhong X, Yin W, Li F, Jiang J (2021) [Effect of salt intake on residual renal function in rats receiving peritoneal dialysis]. Nan Fang Yi Ke Da Xue Xue Bao 41(2):264-271. doi: 10.12122/j.issn.1673-4254.2021.02.15

Objective: To assess the effect of salt intake on residual renal function in rats and explore the possible mechanism.

Summary: In rats with chronic renal failure, high salt intake promotes the activation of the renal RAS system, increases blood pressure, and aggravates renal fibrosis to accelerate the decline of residual renal function, and peritoneal dialysis partially reduces the damage of residual renal function induced by high-salt diets by removing excessive sodium.

Usage: Western Blot.

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Leech extract: A candidate cardioprotective against hypertension-induced cardiac hypertrophy and fibrosis

Wang CH, Pandey S, Sivalingam K, Shibu MA, Kuo WW, Yu-LanYeh, Viswanadha VP, Lin YC, Liao SC, Huang CY (2021) Leech extract: A candidate cardioprotective against hypertension-induced cardiac hypertrophy and fibrosis. J Ethnopharmacol 264:113346. doi: 10.1016/j.jep.2020.113346

Objective: To delineate the molecular mechanisms of medicinal leech extract in the treatment of cardiac hypertrophy and fibrosis, using both in vitro and in vivo assessments.

Summary: Pre-treatment with leech extract significantly reduced angiotensin II (ANG II)-induced cardiac hypertrophy and fibrosis.

Usage: Western blot; PVDF membranes were incubated with Anti-AT-1R.

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Comparative effects of angiotensin II on the contractility of muscularis mucosae and detrusor in the pig urinary bladder.

Lim I, Mitsui R, Kameda M, Sellers DJ, Chess‐Williams R, Hashitani H (2021) Comparative effects of angiotensin II on the contractility of muscularis mucosae and detrusor in the pig urinary bladder. Neurourol Urodyn 40:102-111. doi: 10.1002/nau.24548

Objective: To explore contractile actions of angiotensin II (AT2) on the muscularis mucosae (MM) of the bladder.

Summary: The MM appears to have great sensitivity to AT2, suggesting that MM may be the predominant target of contractile actions induced by AT2 in the bladder.

Usage: Induction of contractions with Anti-AT2R (1 nM – 1 µM). Immunofluorescence (10-100 pM).

Related Products: Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

Characterization of the renin-angiotensin system in aged cavernosal tissue and its role in penile fibrosis.

Bragina ME, Costa-Fraga F, Sturny M, Ebadi B, Ruoccolo RT, Santos RAS, Fraga-Silva RA, Stergiopulos N (2020) Characterization of the renin-angiotensin system in aged cavernosal tissue and its role in penile fibrosis. J Sex Med 17:2129–2140. doi: 10.1016/j.jsxm.2020.08.008

Objective: To investigate local regulation of the renin-angiotensin system (RAS) in age-related erectile dysfunction (ED).

Summary: Experimental results highlighted the essential role of the RAS in modulation of cavernosal fibrosis.

Usage: Immunohistochemistry (1:800)

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Sulfatase 1 mediates IL-10-induced dimethylarginine dimethylaminohydrolase-1 expression and antiproliferative effects in vascular smooth muscle cells of spontaneously hypertensive rats

Kim HY, Kim HS (2021) Sulfatase 1 mediates IL-10-induced dimethylarginine dimethylaminohydrolase-1 expression and antiproliferative effects in vascular smooth muscle cells of spontaneously hypertensive rats. Cytokine 137:155344. doi: 10.1016/j.cyto.2020.155344

Objective: To examine the effects of exSulfs on IL-10-induced dimethylarginine dimethylaminohydrolase-1 (DDAH-1) expression, abrogation of Ang II-induced DDAH-1 downregulation, and inhibition of Ang II-induced proliferation of spontaneously hypertensive rats (SHRs) vascular smooth muscle cells (VSMC). IL-10-induced DDAH-1 expression and abrogation of Ang II-induced DDAH-1 downregulation were attenuated in Sulf1 siRNA-transfected SHRs VSMC.

Summary: Sulf1, and not Sulf2, mediates the IL-10-induced inhibition of Ang II-induced hypertensive effects in SHRs VSMC.

Usage: Western blot (1:800)

Related Products: Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

Endurance exercise intervention is beneficial to kidney function in a rat model of isolated abdominal venous congestion: A pilot study

Cops J, De Moor B, Haesen S, Lijnen L, Wens I, Lemoine L, Reynders C, Penders J, Lambrichts I, Mullens W, Hansen D (2020) Endurance exercise intervention is beneficial to kidney function in a rat model of isolated abdominal venous congestion: A pilot study. J Cardiovasc Transl Res 13(5):769-782. doi: 10.1007/s12265-019-09947-5

Objective: To the effects of moderate intense endurance exercise on heart and kidney function and morphology in a rat model of abdominal venous congestion.

Summary: The implementation of moderate intense endurance exercise reduces abdominal venous pressure and is beneficial to kidney function.

Usage: estern blot; to investigate whole kidney renin-angiotensin-aldosterone system (RAAS) protein expression, the membrane was incubated overnight at 4 °C in the presence of AT-1R antibody 1:2000

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Hydrogen sulfide attenuated Angiotensin II-induced sympathetic excitation in offspring of renovascular hypertensive rats

Feng X, Guo Q, Xue H, Duan X, Jin S, Wu Y (2020) Hydrogen sulfide attenuated Angiotensin II-induced sympathetic excitation in offspring of renovascular hypertensive rats. Front Pharmacol 11:565726. doi: 10.3389/fphar.2020.565726

Objective: To whether sensitivity to angiotensin II (Ang II) is enhanced in offspring of renovascular hypertensive animals and whether hydrogen sulfide (H2S) can attenuate the increased response to Ang II in offspring.

Summary: Prenatal or postnatal administration of H2S attenuated AngII-induced sympathetic excitation in offspring of hypertensive rats.

Usage: Western blot (1:800)

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Effects of adjuvant-induced arthritis on the ventral prostate of rats treated with angiotensin AT1 receptor blocker

da Rocha ES, Gomes FC, de Oliveira MR, Bruneto RV, Borges RMR, de Carvalho EP, Angeli-Junior EV, Garcia-Filho GF, Doná JPL, de Oliveira Batanero RP, Carlos CP, de Melo-Neto JS (2020) Effects of adjuvant-induced arthritis on the ventral prostate of rats treated with angiotensin AT1 receptor blocker. Life Sci 252:117629. doi: 10.1016/j.lfs.2020.117629

Objective: To analyze the prostatic compartments, extracellular matrix, microvascularization, transforming growth factor-beta (TGF-β) and angiotensin II receptors type 1 (AT1) levels, and histopathology of the ventral prostate in a rat model for rheumatoid arthritis, and to evaluate the effect of angiotensin II AT1 receptor blocker (ARB) in the disease.

Summary: AIA may activate specific mechanisms that modify the prostatic environment; the use of ARB attenuates some altered prostate parameters in a rat model for arthritis.

Usage: Immunohistochemistry (1:500)

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Activation of the renin-angiotensin system in high fructose-induced metabolic syndrome.

Kim M, Do GY, Kim I (2020) Activation of the renin-angiotensin system in high fructose-induced metabolic syndrome. Korean J Physiol Pharmacol 24(4):319-328. doi: 10.4196/kjpp.2020.24.4.319

Objective: To investigate the hypothesis that high fructose intake induces activation of the renin-angiotensin systems (RAS), resulting in hypertension and metabolic syndrome.

Summary: High fructose intake increased serum renin, Ang II, triglyceride, and cholesterol levels. High fructose intake increased the expression of angiotensinogen in the liver; angiotensin-converting enzyme in the lungs; and renin, angiotensin II type 1a receptor (AT1aR), and angiotensin II type 1b receptor (AT1bR) in the kidneys. High fructose intake induces activation of RAS, resulting in hypertension and metabolic syndrome.

Usage: Immunohistochemistry

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Vascular-cognitive impairment after chronic high-thoracic spinal cord injury

Jia M (2020) Vascular-cognitive impairment after chronic high-thoracic spinal cord injury. University of British Columbia, Experimental Medicine Thesis.

Objective: To investigate whether chronic disruption of cardiovascular control following experimental spinal cord injury (SCI) results in cerebrovascular decline and vascular cognitive impairment.

Summary: Chronic experimental SCI is associated with structural alteration and endothelial dysfunction in cerebral arteries that likely contributes to significantly reduced CBF and vascular cognitive impairment.

Usage: Immunofluorescence (1:600)

Related Products: Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

The role of the renin-angiotensin system in regulating endometrial neovascularization during the peri-implantation period: literature review and preliminary data

Qi R, Li TC, Chen X (2020) The role of the renin-angiotensin system in regulating endometrial neovascularization during the peri-implantation period: literature review and preliminary data. Ther Adv Endocrinol Metab 11:2042018820920560. doi: 10.1177/2042018820920560

Objective: The role of the renin–angiotensin system (RAS) in the peri-implantation endometrium.

Summary: Altered vasculature of the endometrium in the peri-implantation period is detrimental to implantation and may lead to recurrent miscarriage. Being an angiogenic mediator, endometrial RAS may play a role around the time of embryo implantation, affecting subsequent pregnancy outcomes.

Usage: Immunohistochemistry (1:100)

Related Products: Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

Baoyuan decoction ameliorates apoptosis via AT1-CARP signaling pathway in H9C2 cells and heart failure post-acute myocardial infarction rats

Wang X, Meng H, Wang Q, Shao M, Lu W, Chen X, Jiang Y, Li C, Wang Y, Tu P (2020) Baoyuan decoction ameliorates apoptosis via AT1-CARP signaling pathway in H9C2 cells and heart failure post-acute myocardial infarction rats. J Ethnopharmacol 252:112536. doi: 10.1016/j.jep.2019.112536

Objective: To elucidate whether Baoyuan decoction (BYD) ameliorates apoptosis to protect against heart failure via the AT1-Cardiac ankyrin repeat protein (CARP) signaling pathway.

Summary: BYD improves cardiac function and protects against cardiomyocytes injury by inhibiting apoptosis via regulating the AT1-CARP signaling pathway.

Usage: Western blot (1:1000)

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

The regulation effect of WNT-RAS signaling in hypothalamic paraventricular nucleus on renal fibrosis.

Zhou G, Li J, Zeng T, Yang P, Li A (2020) The regulation effect of WNT-RAS signaling in hypothalamic paraventricular nucleus on renal fibrosis. J Nephrol 33(2):289-297. doi: 10.1007/s40620-019-00637-8

Objective: To investigate whether abnormal expression of central wnt/β-catenin signaling and renin-angiotensin system (WNT-RAS signaling) was tightly involved in chronic kidney disease (CKD).

Summary: These data suggest that the central WNT-RAS signaling plays a significant role in the development and progression of CKD.

Usage: immunohistochemistry and western blot

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Seminal levels of angiotensin II and angiotensin II type 2 receptor expression on spermatozoa in varicocele patients: Relation to fertility status

El-Ariny AF, El-Garem YF, Eid AA, Donia HM, Aly RG, Abdelmeniem IM (2020) Seminal levels of angiotensin II and angiotensin II type 2 receptor expression on spermatozoa in varicocele patients: Relation to fertility status. Andrologia 52(3):e13513. doi: 10.1111/and.13513

Objective: To study the levels of angiotensin II in semen and angiotensin II type 2 receptor expression on spermatozoa in varicocele patients in relation to their fertility status and to evaluate the influence of varicocelectomy on their levels in infertile varicocele patients.

Summary: Dysregulation of angiotensin II and angiotensin II type 2 receptor in varicocele patients may be involved in varicocele-related infertility.

Usage: Immunocytochemistry (1:15)

Related Products: Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

Angiotensin II type 1 receptor blockers favorably affect renal angiotensin II and MAS receptor expression in patients with diabetic nephropathy

Ma YP, Yang Y, Jiang SM, Liu L, Zhang Z, Wang YN, Zou GM, Li WG (2020) Angiotensin II type 1 receptor blockers favorably affect renal angiotensin II and MAS receptor expression in patients with diabetic nephropathy. J Renin Angiotensin Aldosterone Syst 21(2):1470320320919607. doi: 10.1177/1470320320919607

Objective: To assess the renal expression of angiotensin II type 1 receptor (AT1R), angiotensin II type 2 receptor (AT2R), and MAS receptor in human type 2 diabetic nephropathy (DN).

Summary: Renal expression levels of AT1R increase during the early stages of DN, ARB reducing AT1R while increasing MAS receptor. Therefore, ARB should be used as soon as possible in patients with DN.

Usage: Immunohistochemistry

Related Products: Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

Fugan Wan alleviates hepatic fibrosis by inhibiting ACE/Ang II/AT-1R signaling pathway and enhancing ACE2/Ang 1-7/Mas signaling pathway in hepatic fibrosis rat models

Li S, Zhao W, Tao Y, Liu C (2020) Fugan Wan alleviates hepatic fibrosis by inhibiting ACE/Ang II/AT-1R signaling pathway and enhancing ACE2/Ang 1-7/Mas signaling pathway in hepatic fibrosis rat models. Am J Transl Res 12(2):592-601.

Objective: To investigate protective effects of Fugan Wan (FGW) on hepatic fibrosis and clarify associated mechanisms.

Summary: FGW alleviated hepatic fibrosis by inhibiting ACE/Ang II/AT-1R signaling and enhancing ACE2/Ang 1-7/Mas signaling pathway in hepatic fibrosis rat models.

Usage: Western blot

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Chronic treatment with apelin, losartan and their combination reduces myocardial infarct size and improves cardiac mechanical function

Abbasloo E, Najafipour H, Vakili A (2020) Chronic treatment with apelin, losartan and their combination reduces myocardial infarct size and improves cardiac mechanical function. Clin Exp Pharmacol Physiol 47:393-402. doi: 10.1111/1440-1681.13195

Objective: To determine the effect of chronic pretreatment with apelin, losartan and their combination on ischemia-reperfusion (IR) injury in the isolated perfused rat heart and on the expression of apelin-13 receptor (APJ) and angiotensin type 1 receptor (AT1R) in the myocardium.

Summary: Chronic pretreatment with apelin along with AT1R antagonist had more protective effects against IR injury.

Usage: 1:10000

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Attenuation of angiotensin II–induced hypertension in BubR1 low‐expression mice via repression of angiotensin II receptor 1 overexpression.

Aoyagi Y, Furuyama T, Inoue K, Matsuda D, Matsubara Y, Okahara A, Ago T, Nakashima Y, Mori M, Matsumoto T (2019) Attenuation of angiotensin II–induced hypertension in BubR1 low‐expression mice via repression of angiotensin II receptor 1 overexpression. J Am Heart Assoc 8(23):e011911. doi: 10.1161/JAHA.118.011911

Usage: western blot

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Qishen Granule improved cardiac remodeling via balancing M1 and M2 macrophages

Lu W, Wang Q, Sun X, He H, Wang Q, Wu Y, Liu Y, Wang Y, Li C (2019) Qishen Granule improved cardiac remodeling via balancing M1 and M2 macrophages. Front Pharmacol 10:1399. doi: 10.3389/fphar.2019.01399

Objective: To explore the effect of Qishen Granule (QSG) on the release of splenic monocytes, the recruitment of monocytes into heart tissues and the differentiation of macrophages under ischemic conditions.

Summary: Compounds of Chinese medicine have synergistic effects on cardiac and splenic organs through regulating differentiation of monocytes/macrophages in inhibiting myocardial remodeling.

Usage: Western blot

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Targeting AngII/AT1R signaling pathway by perindopril inhibits ongoing liver fibrosis in rat.

Abd El‐Rahman SS, Fayed HM (2019) Targeting AngII/AT1R signaling pathway by perindopril inhibits ongoing liver fibrosis in rat. J Tissue Eng Regen Med 13:2131-2141. doi: 10.1002/term.2940

Usage: IHC (1:100)

Related Products: Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

Systemic ß adrenergic stimulation/ sympathetic nerve system stimulation influences intraocular RAS through cAMP in the RPE

Martins JR, Reichhart N, Kociok N, Stindl J, Foeckler R, Lachmann P, Todorov V, Castrop H, Strauß O (2019) Systemic ß adrenergic stimulation/ sympathetic nerve system stimulation influences intraocular RAS through cAMP in the RPE. Exp Eye Res 189:107828. doi: 10.1016/j.exer.2019.107828

Objective: To investigate whether systemic β-adrenergic stimulation of the retinal pigment epithelium (RPE) also modulates renin expression in the RPE.

Summary: In vitro analysis of renin gene expression using polarized porcine RPE showed that the activity of the renin promoter can be increased by cAMP stimulation (IBMX/forskolin) but was not influenced by angiotensin-2.

Usage: Immunohistochemistry; eye sections were labeled overnight at 4°C with Anti-AT-1R.

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

LncRNA AK094457 promotes AngII-mediated hypertension and endothelial dysfunction through suppressing of activation of PPARγ.

Zhuo X, Wu Y, Yang Y, Gao L, Qiao X, Chen T (2019) LncRNA AK094457 promotes AngII-mediated hypertension and endothelial dysfunction through suppressing of activation of PPARγ. Life Sci 233:116745. doi: 10.1016/j.lfs.2019.116745

Objective: To find the functional lncRNAs associated with hypertension, high throughput lncRNA microarray were used to analyze expression profile of the lncRNAs in the aortic vascular endothelial cells of spontaneously hypertensive rats.

Summary: lncRNA AK094457 is identified as a key regulator in blood pressure and endothelial function, which can increase AngII-induced hypertension and endothelial dysfunction via suppression of PPARγ.

Usage: Western Blot (1:500), immunohistochemistry

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Mixed vehicle emissions induces angiotensin ii and cerebral microvascular angiotensin receptor expression in C57Bl/6 mice and promotes alterations in integrity in a blood-brain barrier coculture model.

Suwannasual U, Lucero J, Davis G, McDonald JD, Lund AK (2019) Mixed vehicle emissions induces angiotensin ii and cerebral microvascular angiotensin receptor expression in C57Bl/6 mice and promotes alterations in integrity in a blood-brain barrier coculture model. Toxicol Sci 170(2):525-535. doi: 10.1093/toxsci/kfz121

Objective: To investigate the hypothesis that inhalation exposure to a mixture of gasoline and diesel vehicle engine emissions (MVE) results in altered central nervous system microvascular integrity mediated by Ang II-AT1 signaling.

Summary: Inhalation exposure to traffic-generated pollutants results in altered BBB integrity, mediated through Ang II-AT1 signaling and inflammation, which is exacerbated by a high-fat diet.

Usage: Antibody was used in immunofluorescent staining of cerebral tissue. Brain sections (10 mm) were prepared for AT1 receptor (1:1000).

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Altered expression of renin-angiotensin system receptors throughout colorectal adenoma-adenocarcinoma sequence.

Beitia M, Solano-Iturri JD, Errarte P, Sanz B, Perez I, Etxezarraga MC, Loizate A, Asumendi A, Larrinaga G (2019) Altered expression of renin-angiotensin system receptors throughout colorectal adenoma-adenocarcinoma sequence. Int J Med Sci 16(6):813-821. doi: 10.7150/ijms.32599

Usage: immunohistochemistry (1:100)

Related Products: Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

Cardiac hypertrophy in mice submitted to a swimming protocol: Influence of training volume and intensity on myocardial renin-angiotensin system.

Soares DdS, Pinto GH, Lopes A, Caetano DSL, Nascimento TG, Andrades ME, Clausell N, Rohde LEP, Leitão SAT, Biolo A (2019) Cardiac hypertrophy in mice submitted to a swimming protocol: Influence of training volume and intensity on myocardial renin-angiotensin system. Am J Physiol Regul Integr Comp Physiol 316(6):R776-R782. doi: 10.1152/ajpregu.00205.2018

Objective: To analyze the cardiac hypertrophy phenotype and the classical and alternative RAS axes in the myocardium of mice submitted to swimming exercises of varying volume and intensity for the development of cardiac hypertrophy.

Summary: Increasing exercise volume and intensity did not induce a pathological hypertrophy phenotype, but instead improved the oxidative capacity, and this process might have the participation of the RAS alternative axis.

Usage: Incubation at 4°C with anti-AT1R diluted in TTBS buffer with 5% non-fat 220 milk 1:800).

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Inhibition of STAT3 activation mediated by toll-like receptor 4 attenuates angiotensin II-induced renal fibrosis and dysfunction

Xu Z, Zou C, Yu W, Xu S, Huang L, Khan Z, Wang J, Liang G, Wang Y (2019) Inhibition of STAT3 activation mediated by toll-like receptor 4 attenuates angiotensin II-induced renal fibrosis and dysfunction. Br J Pharmacol 176(14):2627-2641. doi: 10.1111/bph.14686

Objective: To investigate the underlying mechanisms of STAT3 activation by angiotensin II and downstream functional consequences in the kidneys.

Summary: This study reveals a novel mechanism of STAT3 activation, induced by angiotensin II, in kidney tissues and highlights a translational significance of a STAT3 inhibitor as potential therapeutic agent for hypertensive kidney disease.

Usage: Western blot

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Losartan, an angiotensin II type 1 receptor antagonist, alleviates mechanical hyperalgesia in a rat model of chemotherapy-induced neuropathic pain by inhibiting inflammatory cytokines in the dorsal root ganglia.

Kim E, Hwang S-H, Kim H-K, Abdi S, Kim HK (2019) Losartan, an angiotensin II type 1 receptor antagonist, alleviates mechanical hyperalgesia in a rat model of chemotherapy-induced neuropathic pain by inhibiting inflammatory cytokines in the dorsal root ganglia. Mol Neurobiol 56(11):7408-7419. doi: 10.1007/s12035-019-1616-0

Objective: To assess losartan’s analgesic effect on paclitaxel-induced neuropathic pain (PINP) in rats and its mechanism of action in dorsal root ganglion (DRG).

Summary: The mechanical thresholds, protein levels of inflammatory cytokines, and cellular location of AT1R and interleukin 1β (IL-1β) in the DRG were assessed with behavioral testing, Western blotting, and immunohistochemistry, respectively.

Usage: western blot (1:100)

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A.

Im E-J, Lee C-H, Moon P-G, Rangaswamy GG, Lee B, Lee JM, Lee J-C, Jee J-G, Bae J-S, Kwon T-K, Kang K-W, Jeong M-S, Lee J-E, Jung H-S, Ro H-J, Jun S, Kang W, Seo S-Y, Cho Y-E, Song B-J, Baek M-C (2019) Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A. Nature Commun 10(1):1387. doi: 10.1038/s41467-019-09387-4

Objective: To identify a direct protein target of SFX in cancer cells.

Summary: Using data from the BindingDB, SEA identified four proteins as target candidates, which are: endothelin receptor type A, kynurenine 3-monooxygenase, carbonic anhydrase 13, and angiotensin II type 1a receptor.

Usage: Western blot (1:1000).

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Kangxianling decoction prevents renal fibrosis in rats with 5/6 nephrectomy and inhibits Ang II-induced ECM production in glomerular mesangial cells.

Ji J, Tao P, He L (2019) Kangxianling decoction prevents renal fibrosis in rats with 5/6 nephrectomy and inhibits Ang II-induced ECM production in glomerular mesangial cells. J Pharmacol Sci 139(4):367-372. doi: 10.1016/j.jphs.2019.03.003

Objective: To investigate effects of Kangxianling decoction on renal fibrosis in a rat model of 5/6 Nephrectomy (5/6 N) in vivo and in angiotensin II (Ang II)-treated rat glomerular mesangial cells (HBZY-1) in vitro.

Summary: Kangxianling decoction could reduce renal fibrosis, accompanied by inhibiting the production of ECM proteins and JNK, along with downregulation of TGF-β, Ang II.

Usage: Western blot analysis (1:100).

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Inhibition of methamphetamine self-administration and reinstatement by central blockade of angiotensin II receptor in rats.

Xu X, Pan J, Li X, Cui Y, Mao Z, Wu B, Xu H, Zhou W, Liu Y (2019) Inhibition of methamphetamine self-administration and reinstatement by central blockade of angiotensin II receptor in rats. J Pharmacol Exp Ther 369(2):244-258. doi: 10.1124/jpet.118.255729

Objective: To explore the role of central angiotensin II receptor (ATR) in drug taking and seeking behavior associated with methamphetamine (METH) use disorder.

Summary: The AT1R-PLCβ-CREB signaling pathway was found to be associated with the effect of AT1R on the drug-taking and drug-seeking behavior involving METH use disorder.

Usage: Membranes were incubated at 4°C overnight (1:1000). Western blot.

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Infrared fluorescence imaging of infarcted hearts with Ag2S nanodots.

Ortgies DH, García-Villalón ÁL, Granado M, Amor S, Rodríguez EM, Santos HDA, Yao J, Rubio-Retama J, Jaque D (2019) Infrared fluorescence imaging of infarcted hearts with Ag2S nanodots. Nano Res 12:749–757. doi: 10.1007/s12274-019-2280-4

Objective: To investigate the potential application of functionalized Ag2S nanodots for accurate imaging of damaged myocardium tissues after a myocardial infarction induced by either partial or global ischemia.

Summary: NIR-II images generated by ATII-functionalized Ag2S nanodots contain valuable information about the location and extension of damaged tissue in the myocardium allowing for a proper identification of the occluded artery as well as an indirect evaluation of the damage level.

Usage: Assessment of AT1R protein content in ischemic and non-ischemic hearts by Western blot (1:1000).

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Selective abdominal venous congestion induces adverse renal and hepatic morphological and functional alterations despite a preserved cardiac function

Cops J, Mullens W, Verbrugge FH, Swennen Q, De Moor B (2018) Selective abdominal venous congestion induces adverse renal and hepatic morphological and functional alterations despite a preserved cardiac function. Sci Rep 8:17757. doi: 10.1038/s41598-018-36189-3

Objective: The consequences of isolated abdominal venous congestion on morphology and function of the kidneys, liver and heart were studied in a rat model.

Summary: This study provides relevant insight in the pathophysiology of abdominal congestion on organ function.

Usage: Western blot. PVDF membrane was incubated overnight at 4 °C in the presence of antibody (1/2000).

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Nanoparticle-mediated delivery of Tanshinone IIA reduces adverse cardiac remodeling following myocardial infarctions in a mice model: Role of NF-κB pathway

Mao S, Wang L, Chen P, Lan Y, Guo R, Zhang M (2018) Nanoparticle-mediated delivery of Tanshinone IIA reduces adverse cardiac remodeling following myocardial infarctions in a mice model: Role of NF-κB pathway. Artif Cells Nanomed Biotechnol 46(sup3):S707-S716. doi: 10.1080/21691401.2018.1508028

Objective: To develop a nanoparticle-mediated drug delivery system for Tanshinone IIA.

Summary: Demonstrated a novel delivery mechanism of a therapeutic agent, tanshinone IIA-NP, which prevented IκB phosphorylation and subsequent NF-κB activation resulting in the suppression of inflammatory responses and reduction of cardiomyocytes apoptosis and fibrotic process, contributing to the mitigation of LV remodeling and improvement of cardiac functions following MI.

Usage: Immunoblotting analysis for the activation of AT-1R on an ischemic border zone 4 weeks after explosion to coronary artery ligation.

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

The angiotensin II type 2 receptors protect renal tubule mitochondria in early stages of diabetes mellitus

Micakovic T, Papagiannarou S, Clark E, Kuzay Y, Abramovic K, Peters J, Sticht C, Volk N, Fleming T, Nawroth P, Hammes HP, Alenina N, Grone HJ, Hoffmann SC (2018) The angiotensin II type 2 receptors protect renal tubule mitochondria in early stages of diabetes mellitus. Kidney Int 94:937-950. doi: 10.1016/j.kint.2018.06.006

Objective: To investigate the role of the angiotensin II type 2 receptor (AT-2R) at the early stages of diabetic nephropathy.

Summary: Targeted AT-2R overexpression in renal cells may open new avenues to develop novel types of drugs preventing diabetic nephropathy.

Usage: Histology, immunohistochemistry; 1:100

Related Products: Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

Expression of the intrarenal angiotensin receptor and the role of renin-angiotensin system inhibitors in IgA nephropathy.

Zhang Z, Jiang SM, Ma YP, Dai PL, Wang YN, Zou GM, Gao HM, Yang Y, Li WG (2019) Expression of the intrarenal angiotensin receptor and the role of renin-angiotensin system inhibitors in IgA nephropathy. Mol Cell Biochem 453:103-110. doi: 10.1007/s11010-018-3435-4

Objective: To investigate the role of intrarenal renin-angiotensin system (RAS) in the development of human kidney disease. Patients with IgA nephropathy (IgAN) were screened and their clinical characteristics analyzed.

Summary: Overexpression of AT1R on the mesangial cells of IgAN patients is associated with mesangial cell proliferation, glomerular segmental sclerosis, and crescent formation.

Usage: immunohistochemistry (1:500)

Related Products: Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

AT2R activation prevents microglia pro-inflammatory activation in a nox-dependent manner: Inhibition of PKC activation and p47(PHOX) phosphorylation by PP2A.

Bhat SA, Sood A, Shukla R, Hanif K (2019) AT2R activation prevents microglia pro-inflammatory activation in a nox-dependent manner: Inhibition of PKC activation and p47(PHOX) phosphorylation by PP2A. Mol Neurobiol 56(4):3005-3023. doi: 10.1007/s12035-018-1272-9

Objective: To investigate the involvement of AT2R in NADPH oxidase (NOX)-mediated microglia activation.

Summary: AT2R, via PP2A-mediated inhibition of PKC, prevents the NOX activation, ROS generation, and subsequent pro-inflammatory activation of microglia.

Usage: Immunoblotting: membranes were incubated with respective primary antibodies AT1R (1:1000), AT2R (1:500). Immunocytochemistry: AT1R (1:100), AT2R (1:100).

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP), Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

Eriobotrya japonica ameliorates cardiac hypertrophy in H9c2 cardiomyoblast and in spontaneously hypertensive rats

Chiang JT, Badrealam KF, Shibu MA, Kuo CH, Huang CYA-Ohoo, Chen BC, Lin YM, Viswanadha VP, Kuo WW, Huang CY (2018) Eriobotrya japonica ameliorates cardiac hypertrophy in H9c2 cardiomyoblast and in spontaneously hypertensive rats. Environ Toxicol 33:1113-1122. doi: 10.1002/tox.22589

Objective: To evaluate the prospective efficacy of E. japonica leave extract (EJLE) against Angiotensin-II induced cardiac hypertrophy.

Summary: Supplementation of EJLE could rescue Ang-II induced cardiac hypertrophy.

Usage: Western blot, immunohistochemistry

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

The effect and mechanism of chinese herbal formula Sini Tang in heart failure after myocardial infarction in rats

Zhu Y, Zhao J, Han Q, Wang Z, Wang Z, Dong X, Li J, Liu L, Shen X (2018) The effect and mechanism of chinese herbal formula Sini Tang in heart failure after myocardial infarction in rats. Evid Based Complement Alternat Med 2018:5629342. doi: 10.1155/2018/5629342

Objective: To investigate the effectiveness and mechanism of theChinese herbal formula Sini Tang (SNT) in heart failure after myocardial infarction in rats.

Summary: The Chinese herbal formula SNT could improve left ventricular systolic function in heart failure after myocardial infarction.

Usage: Western blot.

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Effect of chronic intermittent hypoxia on angiotensin II receptors in the central nervous system

Morgan BJ, Schrimpf N, Rothman M, Mitzey A, Brownfield MS, Speth RC, Dopp JM (2018) Effect of chronic intermittent hypoxia on angiotensin II receptors in the central nervous system. Clin Exp Hypertens 41:1-7. doi: 10.1080/10641963.2018.1451536

Objective: To quantify the effects of chronic intermittent hypoxia (CIH) on AT1R- and AT2R-like immunoreactivity in the rostroventrolateral medulla (RVLM) and paraventricular nucleus of the hypothalamus (PVN), central regions that are important components of the extended chemoreflex pathway.

Summary: Exposure to CIH of at least 5 days duration augmented AT1R-like immunoreactivity in RVLM. In contrast, CIH exposure did not affect AT2R-like reactivity in RVLM or that of either Ang II receptor subtype in PVN.

Usage: Immunoreactivity, immunostaining. For immunoreactivity assays, antibodies were diluted in the blocking solution with 0.05% sodium azide at the following concentrations: AT-1R, (1:1600) and AT-2R, (1:5000).

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP), Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

Developmental and degenerative cardiac defects in the Taiwanese mouse model of severe spinal muscular atrophy

Maxwell GK, Szunyogova E, Shorrock HK, Gillingwater TH (2018) Developmental and degenerative cardiac defects in the Taiwanese mouse model of severe spinal muscular atrophy. J Anat 232:965-978. doi: 10.1111/joa.12793

Objective: To investigate changes occurring in the heart, predominantly at pre- and early symptomatic ages, in the Taiwanese mouse model of severe Spinal muscular atrophy (SMA).

Summary: The findings support the requirement to develop systemic therapies for SMA capable of treating non-neuromuscular pathologies.

Usage: Immunohistochemistry, Western blot

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Erythropoietin alleviates post-resuscitation myocardial dysfunction in rats potentially through increasing the expression of angiotensin II receptor type 2 in myocardial tissues

Zhou H, Huang J, Zhu L, Cao Y (2018) Erythropoietin alleviates post-resuscitation myocardial dysfunction in rats potentially through increasing the expression of angiotensin II receptor type 2 in myocardial tissues. Mol Med Rep 17:5184-5192. doi: 10.3892/mmr.2018.8473

Objective: To determine whether erythropoietin (EPO) improves post‑resuscitation myocardial dysfunction and how it affects the renin‑angiotensin system.

Summary: The present study confirmed that EPO treatment is beneficial for protecting cardiac function post‑resuscitation, and the roles of EPO in alleviating post‑resuscitation myocardial dysfunction may potentially be associated with enhanced myocardial expression of AT2R.

Usage: Western blot analysis of AT-1R (1:500) in the myocardium

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Attenuation of the infiltration of angiotensin ii expressing CD3+ T-cells and the modulation of nerve growth factor in lumbar dorsal root ganglia – a possible mechanism underpinning analgesia produced by EMA300, an Angiotensin II type 2 (AT2) receptor antagonist

Khan N, Muralidharan A, Smith MT (2017) Attenuation of the infiltration of angiotensin ii expressing CD3+ T-cells and the modulation of nerve growth factor in lumbar dorsal root ganglia - a possible mechanism underpinning analgesia produced by EMA300, an Angiotensin II type 2 (AT2) receptor antagonist. Front Mol Neurosci 10:389. doi: 10.3389/fnmol.2017.00389

Objective: To investigate the cellular and molecular mechanism of action of selective small molecule angiotensin II type 2 (AT2) receptor antagonists in the alleviation of peripheral neuropathic pain.

Summary: The analgesic effect of EMA300 in CCI-rats involves multimodal actions that appear to be mediated at least in part by a significant reduction in the otherwise increased expression levels of Ang II as well as the number of Ang II-expressing CD3+ T-cells in the ipsilateral lumbar DRGs of CCI-rats.

Usage: Immunocytochemistry; specifically labeled HEK cells expressing the AT2 but not the AT1 receptor or the non-transfected HEK-cells.

Related Products: Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

Angiotensin II induces monocyte chemoattractant protein-1 expression by increasing reactive oxygen species-mediated activation of the nuclear factor‑κB signaling pathway in osteoblasts

Wang C, Zhang C, Zhou F, Gao L, Wang Y, Wang C, Zhang Y (2018) Angiotensin II induces monocyte chemoattractant protein-1 expression by increasing reactive oxygen species-mediated activation of the nuclear factor‑κB signaling pathway in osteoblasts. Mol Med Rep 17:1166-1172. doi: 10.3892/mmr.2017.7971

Objective: To investigate the effect of angiotensin II (Ang II) on monocyte chemoattractant protein‑1 (MCP‑1) expression and the underlying mechanism in osteoblasts.

Summary: These data indicate that Ang II-enhanced ROS production and activated NF‑κB signaling via AT1R, thus upregulating MCP‑1 expression in osteoblasts.

Usage: Western Blot; the membranes were blocked with 5% fat‑free milk and probed with anti‑AT1R (1:600).

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

Angiotensin II’s role in sodium lactate-induced panic-like responses in rats with repeated urocortin 1 injections into the basolateral amygdala: amygdalar angiotensin receptors and panic

Johnson PL, Sajdyk TJ, Fitz SD, Hale MW, Lowry CA, Hay-Schmidt A, Shekhar A (2013) Angiotensin II's role in sodium lactate-induced panic-like responses in rats with repeated urocortin 1 injections into the basolateral amygdala: amygdalar angiotensin receptors and panic. Prog Neuropsychopharmacol Biol Psychiatry 44:248-256. doi: 10.1016/j.pnpbp.2013.02.014

Objective: To test the hypothesis that the subfornical organ (SFO) projects to the basolateral amygdala (BLA) and releases angiotensin-II (A-11) to mobilize panic responses.

Summary: The presence of AT-1R was confirmed in the BLA which, combined with previous data, suggests that A-II’s panicogenic effects in the BLA are AT-1R dependent.

Usage: Immunohistochemistry (1:250 to 1:500).

Related Products: Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

A small molecule angiotensin II type 2 receptor (AT₂R) antagonist produces analgesia in a rat model of neuropathic pain by inhibition of p38 mitogen-activated protein kinase (MAPK) and p44/p42 MAPK activation in the dorsal root ganglia

Smith MT, Woodruff TM, Wyse BD, Muralidharan A, Walther T (2013) A small molecule angiotensin II type 2 receptor (AT₂R) antagonist produces analgesia in a rat model of neuropathic pain by inhibition of p38 mitogen-activated protein kinase (MAPK) and p44/p42 MAPK activation in the dorsal root ganglia. Pain Med 14(10):1557-1568. doi: 10.1111/pme.12157

Objective: To elucidate the mechanism through which EMA300, a small molecule antagonist of the angiotensin II type 2 receptor (AT2R) with >1,000-fold selectivity over the angiotensin II type 1 receptor, produces analgesia in a rodent model of neuropathic pain.

Summary: Augmented angiotensin II/AT2R signaling in the DRGs of CCI rats is attenuated by EMA300 to block p38 MAPK and p44/p42 MAPK activation, a mechanism with clinical validity for alleviating neuropathic pain.

Usage: Immunostaining (1:250), rat DRG sections

Related Products: Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

Immunohistochemical localization of AT1a, AT1b, and AT2 Angiotensin II receptor subtypes in the rat adrenal, pituitary, and brain with a perspective commentary.

Premer C, Lamondin C, Mitzey A, Speth R, Brownfield M (2013) Immunohistochemical localization of AT1a, AT1b, and AT2 Angiotensin II receptor subtypes in the rat adrenal, pituitary, and brain with a perspective commentary. Int J Hypertens 2013:175428. doi: 10.1155/2013/175428

Objective: To immunohistochemically differentiate the angiotensin receptor subtypes: AT-1ARa, AT-1ARb, and AT-2R.

Summary: Antibodies that can differentiate the three different angiotensin II receptor subtypes in the rat were used to immunohistochemically label angiotensin II receptor subtype-like immunoreactivity in the rat adrenal, pituitary, and brain. The pattern of staining corroborates mRNA, radioligand binding, and functional studies of adrenal and anterior pituitary angiotensin receptors.

Usage: Western blot (1:500), immunofluorescence (1:500), immunocytochemistry (AB-N25AP and AB-N26AP at 1:500, AB-N28AP at 1:2000).

Related Products: Angiotensin II receptor (AT-1AR) Rabbit Polyclonal, affinity-purified (Cat. #AB-N25AP), Angiotensin II receptor (AT-1BR) Rabbit Polyclonal, affinity-purified (Cat. #AB-N26AP), Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

Angiotensin-II is a putative neurotransmitter in lactate-induced panic-like responses in rats with disruption of GABAergic inhibition in the dorsomedial hypothalamus.

Shekhar A, Johnson PL, Sajdyk TJ, Fitz SD, Keim SR, Kelley PE, Gehlert DR, DiMicco JA (2006) Angiotensin-II is a putative neurotransmitter in lactate-induced panic-like responses in rats with disruption of GABAergic inhibition in the dorsomedial hypothalamus. J Neurosci 26(36):9205-9215. doi: 10.1523/JNEUROSCI.2491-06.2006

Objective: To determine the exact mechanism for lactate to elicit a panic attack.

Summary: Results implicate A-II pathways and the A-II receptors (AT-1Ra, AT-1Rb) in the hypothalamus as putative substrates for sodium lactate-induced panic-like responses in vulnerable subjects.

Usage: Immunohistochemistry (1:500), Western blot (1:500)

Related Products: Angiotensin II receptor (AT-1AR) Rabbit Polyclonal, affinity-purified (Cat. #AB-N25AP), Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP)

NADPH oxidase contributes to angiotensin II signaling in the nucleus tractus solitarius.

Wang G, Anrather J, Huang J, Speth RC, Pickel VM, Iadecola C (2004) NADPH oxidase contributes to angiotensin II signaling in the nucleus tractus solitarius. J Neurosci 24(24):5516-5524. doi: 10.1523/JNEUROSCI.1176-04.2004

Objective: To investigated whether NADPH oxidase is involved in angiotensin II signaling in central autonomic neurons.

Summary: Angiotensin II (AngII), acting through angiotensin type 1 (AT1 ) receptors, exerts powerful effects on central autonomic networks regulating cardiovascular homeostasis and fluid balanceColocalization of AT-1r’s and a NADPH oxidase subunit provides evidence that NADPH oxidase is involved in the effects of angiotensin II on autonomic neurons.

Usage: Immunolabeling (1:200)

Related Products: Angiotensin II receptor (AT-1AR) Rabbit Polyclonal, affinity-purified (Cat. #AB-N25AP), Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP), Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

Angiotensin II subtype 1A (AT1A) receptors in the rat sensory vagal complex: Subcellular localization and association with endogenous angiotensin.

Huang J, Hara Y, Anrather J, Speth RC, Iadecola C, Pickel VM (2003) Angiotensin II subtype 1A (AT1A) receptors in the rat sensory vagal complex: Subcellular localization and association with endogenous angiotensin. Neuroscience 122(1):21-36. doi: 10.1016/s0306-4522(03)00606-7

Objective: To determine interactive sites in the sensory vagal complex of rat brain using dual immunolabeling of antisera recognizing the AT1-AR and Ang II.

Summary: In the rat sensory vagal complex, AT-1A receptors are strategically positioned for involvement in modulation of the postsynaptic excitability and intracrine hormone-like effects of Ang II. In addition, these receptors have distributions consistent with diverse roles in regulation of transmitter release, regional blood flow and/or vascular permeability.

Usage: Immunolabeling (1:200)

Related Products: Angiotensin II receptor (AT-1AR) Rabbit Polyclonal, affinity-purified (Cat. #AB-N25AP), Angiotensin II receptor (AT-1R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N27AP), Angiotensin II receptor (AT-2R) Rabbit Polyclonal, affinity-purified (Cat. #AB-N28AP)

Immunohistochemical localization of AT-1A and AT-1B angiotensin II receptor subtypes in the rat adrenal

Speth RC, Grove KL, Brownfield MS (2001) Immunohistochemical localization of AT-1A and AT-1B angiotensin II receptor subtypes in the rat adrenal. Endocrine Society ENDO 2001 - 83rd Annual Meeting of the Endocrine Society, Denver, Colorado.

Related Products: Angiotensin II receptor (AT-1AR) Rabbit Polyclonal, affinity-purified (Cat. #AB-N25AP)