NO-L-Cysteine Mouse Monoclonal References

NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)

23 entries found for : ab-t125

Posttranslational S-nitrosylation modification regulates HMGB1 secretion and promotes its proinflammatory and neurodegenerative effects

Yang R, Gao Y, Li H, Huang W, Tu D, Yang M, Liu X, Hong JS, Gao HM (2022) Posttranslational S-nitrosylation modification regulates HMGB1 secretion and promotes its proinflammatory and neurodegenerative effects. Cell Rep 40(11):111330. doi: 10.1016/j.celrep.2022.111330

Objective: To demonstrate that S-nitrosylation (SNO) is essential and sufficient for inflammation-elicited HMGB1 secretion.

Summary: Results indicate crucial roles for SNO modification in HMGB1 secretion and HMGB1-Mac1 interaction for inflammatory neurodegeneration, identifying a mechanistic basis for Parkinson’s Disease development.

Usage: Confocal double-label immunofluorescence (1:1000)

Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)

Stimulation of Akt phosphorylation and glucose transport by metalloporphyrins with peroxynitrite decomposition catalytic activity

Eccardt AM, Pelzel RJ, Bell TP, Fisher JS (2022) Stimulation of Akt phosphorylation and glucose transport by metalloporphyrins with peroxynitrite decomposition catalytic activity. Catalysts 12(8):849. doi: 10.3390/catal12080849

Objective: To determine whether a catalytic action of iron porphyrin compounds would be related to their stimulation of insulin signaling and glucose uptake in C2C12 myotubes.

Summary: Findings suggest that iron porphyrin compounds with both peroxynitrite decomposition activity and peroxidase activity can stimulate insulin signaling and glucose transport in skeletal muscle cells.

Usage: Western blot

Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)

Characterizing SERCA function in the hippocampal and prefrontal cortex regions of the brain from C57 and D2 mdx mice

Copeland EN (2022) Characterizing SERCA function in the hippocampal and prefrontal cortex regions of the brain from C57 and D2 mdx mice. Brock University St. Catharines, Ontario, Canada Thesis.

Objective: To investigate the differences between two models of Duchenne muscular dystrophy (DMD): D2 mouse model with young mice showing cognitive deficits, and C57, the traditional mouse model.

Summary: Impaired sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA) function in both C57 and D2 mdx brain – perhaps due to heightened oxidative/nitrosative stress.

Usage: Western blot; NO-L-Cysteine Mouse Monoclonal, Conjugated as a marker of oxidative/nitrosative stress.

Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)

P2X7 receptor augments LPS-induced nitrosative stress by regulating Nrf2 and GSH levels in the mouse hippocampus

Lee DS, Kim JE (2022) P2X7 receptor augments LPS-induced nitrosative stress by regulating Nrf2 and GSH levels in the mouse hippocampus. Antioxidants (Basel) 11(4):778. doi: 10.3390/antiox11040778

Objective: To elucidate whether P2X7R affects nuclear factor-erythroid 2-related factor 2 (Nrf2) activity/expression and glutathione (GSH) synthesis under nitrosative stress in response to lipopolysaccharide (LPS)-induced neuroinflammation.

Summary: The findings indicate that P2X7R may augment LPS-induced neuroinflammation by leading to Nrf2 degradation, aberrant glutamate-glutamine cycle, and impaired cystine/cysteine uptake, which would inhibit GSH biosynthesis.

Usage: Immunohistochemistry (1:1000)

Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)

Sarcoplasmic reticulum Ca2+ handling in young DBA/2J vs C57BL/10 mdx models of Duchenne muscular dystrophy

Cleverdon REG, Whitley KC, Marko DM, Hamstra SI, Braun JL, Roy BD, MacPherson REK, Fajardo VA (2021) Sarcoplasmic reticulum Ca2+ handling in young DBA/2J vs C57BL/10 mdx models of Duchenne muscular dystrophy. bioRxiv 2021.10.25.465805. doi: 10.1101/2021.10.25.465805

Objective: To investigate whether sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA) function would differ in muscles from young D2 and C57 mdx mice.

Summary: The study demonstrates that SERCA function is drastically impaired in young D2 mdx mice.

Usage: Western blot

Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)

Characterizing SERCA function in murine skeletal muscles after 35-37 days of spaceflight

Braun JL, Geromella MS, Hamstra SI, Messner HN, Fajardo VA (2021) Characterizing SERCA function in murine skeletal muscles after 35-37 days of spaceflight. Int J Mol Sci 22(21):11764. doi: 10.3390/ijms222111764

Objective: To investigate SERCA function, SERCA regulatory protein content, and reactive oxygen/nitrogen species (RONS) protein adduction in murine skeletal muscle after 35–37 days of spaceflight.

Summary: Spaceflight affects SERCA function differently between the soleus and tibialis anterior.

Usage: Western blot (20 mg)

Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)

Detection of nitric oxide enzymes and its metabolites

de Sanctis JB (2022) Detection of nitric oxide enzymes and its metabolites. (eds. K Agrawal, J Bouchal, V Das, J Drábek, P Dzubák, M Hajdúch, K Koberna, A Ligasová, M Mistrik, JB de Sanctis, J Srovnal). In: Laboratory Techniques in Cellular and Molecular Medicine 243-252. Palacky University.

Objective: To provide a protocol for detection of nitrotyrosine by ELISA.

Summary: Reiss reaction sulfanilic acid (SA) in the presence of acid medium (phosphoric acid) from the diazonium salt interacts with the azo dye agent, N-alpha-naphthyl-ethylenediamine (NAD), to form a pink color which is read at 540 nm.

Usage: Western blot; detection of S-nitrosocysteine

Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)

Proteome-wide modulation of S-nitrosylation in Trypanosoma cruzi trypomastigotes upon interaction with the host extracellular matrix

Mule SN, Manchola NC, de Oliveira GS, Pereira M, Magalhães RDM, Teixeira AA, Colli W, Alves MJM, Palmisano G (2021) Proteome-wide modulation of S-nitrosylation in Trypanosoma cruzi trypomastigotes upon interaction with the host extracellular matrix. J Proteomics 231:104020. doi: 10.1016/j.jprot.2020.104020

Objective: To map site-specific S-nitrosylated (SNO) proteins from Trypanosoma cruzi trypomastigotes incubated (MTy) or not (Ty) with extracellular matrix (ECM).

Summary: The results provide the first site-specific characterization of S-nitrosylated proteins in T. cruzi and their modulation upon ECM incubation before infection of the mammalian hosts. The reduction of S-nitrosylation upon incubation with ECM, associated with a rewiring of the subcellular distribution and intracellular signaling pathways, was confirmed.

Usage: Indirect Immunofluorescence Assay (1:250)

Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)

Potential roles of neuronal nitric oxide synthase and the PTEN-induced kinase 1 (PINK1)/Parkin pathway for mitochondrial protein degradation in disuse-induced soleus muscle atrophy in adult rats

Uda M, Yoshihara T, Ichinoseki-Sekine N, Baba T, Yoshioka T (2020) Potential roles of neuronal nitric oxide synthase and the PTEN-induced kinase 1 (PINK1)/Parkin pathway for mitochondrial protein degradation in disuse-induced soleus muscle atrophy in adult rats. PLoS One 15(12):e0243660. doi: 10.1371/journal.pone.0243660

Objective: To investigate the involvement of neuronal nitric oxide synthase (nNOS) and the PINK1/Parkin pathway in soleus muscle atrophy induced by 14 days of hindlimb unloading (HU) in adult rats.

Summary: Excessive NO is not produced in atrophied soleus muscles despite nNOS accumulation, suggesting that excessive NO dose not mediate in soleus muscle atrophy at least after 14 days of HU.

Usage: Western blot (1:1000)

Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)

A DNA-based fluorescent probe maps NOS3 activity with subcellular spatial resolution.

Jani MS, Zou J, Veetil AT, Krishnan Y (2020) A DNA-based fluorescent probe maps NOS3 activity with subcellular spatial resolution. Nat Chem Biol 16:660–666. doi: 10.1038/s41589-020-0491-3

Objective: Usage of novel fluorescent DNA-based probe technology to map activities of endogenous NOS3 in live cells provides a platform to discover selective regulators of NOS3 in PM and TGN.

Summary: Targeting of plasma membrane (PM) and trans-Golgi network (TGN) shows that NOS3 is tenfold less active at the Golgi but its activity is essential for the structural integrity of the Golgi.

Usage: immunofluorescence

Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)

Ptps facilitates compartmentalized LTBP1 S-nitrosylation and promotes tumor growth under hypoxia

Zhao Q, Zheng K, Ma C, Li J, Zhuo L, Huang W, Chen T, Jiang Y (2020) Ptps facilitates compartmentalized LTBP1 S-nitrosylation and promotes tumor growth under hypoxia. Mol Cell 77(1):95-107.e5. doi: 10.1016/j.molcel.2019.09.018

Objective: To investigate whether there is a molecular mechanism underlying the regulation of LTBP1-TGF-b signaling by the BH4-biosynthesis pathway.

Summary: GTP cyclohydrolase I (GTPCH), 6-pyruvoyltetrahydropterin synthase (PTPS), and sepiapterin reductase (SR) are sequentially responsible for de novo synthesis of tetrahydrobiopterin (BH4), a known cofactor for nitric oxide synthase (NOS). PTPS metabolic activity is required for colorectal tumor cell growth under hypoxia.

Usage: Immunoblotting

Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)

Waterpipe smoke effect on oxidative stress levels in buccal cells using MAWI i-SWAB tubes

Mouawad J, Azar JA, Bassil M (2019) Waterpipe smoke effect on oxidative stress levels in buccal cells using MAWI i-SWAB tubes. Biomark J 5(1):1-6. doi: 10.36648/2472-1646.5.1.57

Objective: To evaluate the effect of water pipe smoke on oxidative stress levels in buccal cells, using MAWI i-SWAB tubes for the detection of different protein oxidation biomarkers (S-glutathionylation, S-nitrosylation and tyrosine nitration). Furthermore, these tubes were used for the revelation of iNOS and NOX2.

Summary: Results confirmed the findings about the ability of these tubes to detect S-nitrosylation, nitrotyrosine, as well as S-glutathionylation. Results showed also the presence of immature NOX2 in human buccal cells equally.

Usage: Western blot

Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)

Stimulation of brain nicotinic acetylcholine receptors activates adrenomedullary outflow via brain inducible NO synthase-mediated S-nitrosylation

Higashi Y, Shimizu T, Yamamoto M, Tanaka K, Yawata T, Shimizu S, Zou S, Ueba T, Yuri K, Saito M (2018) Stimulation of brain nicotinic acetylcholine receptors activates adrenomedullary outflow via brain inducible NO synthase-mediated S-nitrosylation. Br J Pharmacol 175(19):3758-3772. doi: 10.1111/bph.14445

Objective: To examine central mechanisms for the (±)-epibatidine-induced responses, focusing on brain NOS and NO-mediated mechanisms, soluble GC (sGC) and protein S-nitrosylation (a posttranslational modification of protein cysteine thiol groups), in urethane-anaesthetized (1.0 g·kg1, i.p.) male Wistar rats.

Summary: Stimulation of brain nAChRs can induce elevation of plasma catecholamines through brain iNOS-derived NO-mediated protein Snitrosylation in rats. Therefore, brain nAChRs (at least α4β2 subtype) and NO might be useful targets for alleviation of catecholamines overflow induced by smoking.

Usage: Immunohistochemistry (1:1000)

Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)

Detection of oxidative stress in buccal cells using iSWAB tubes

Bassil M, Atat AA, Ibragim M (2018) Detection of oxidative stress in buccal cells using iSWAB tubes. J Mol Biomark Diagn 9(2):1-6. doi: 10.4172/2155-9929.1000387

Objective: To investigate the oxidative profile of iSWAB-Mawi protein tubes by examining their ability to detect the protein oxidation biomarkers: nitrotyrosine and S-nitrosocysteine in buccal cells.

Summary: Results showed the presence of S-nitrosocysteine and nitrotyrosine in buccal cells reflecting the ability of iSWAB-Mawi protein tubes to detect those protein oxidation biomarkers.

Usage: Western Blot

Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)

Antitumor Potential of S-Nitrosothiol-Containing Polymeric Nanoparticles against Melanoma

Ferraz LS, Watashi CM, Colturato-Kido C, Pelegrino MT, Paredes-Gamero EJ, Weller RB, Seabra AB, Rodrigues T (2018) Antitumor Potential of S-Nitrosothiol-Containing Polymeric Nanoparticles against Melanoma. Mol Pharm 15(3):1160-1168. doi: 10.1021/acs.molpharmaceut.7b01001

Objective: To investigate the molecular mechanisms underlying chitosan nanoparticles containing S-nitrosomercaptosuccinic acid (S-nitroso-MSA-CS) induced cytotoxicity in melanoma cells.

Summary: Melanoma cells were more sensitive to cell death than normal melanocytes. S-Nitroso-MSA-CS-induced cytotoxicity exhibited features of caspase-dependent apoptosis, and it was associated with oxidative stress, characterized by increased mitochondrial superoxide production and oxidation of protein thiol groups.

Usage: Immunostaining (1:200)

Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)

S-Glutathionylation of p47phox sustains superoxide generation in activated neutrophils

Nagarkoti S, Dubey M, Awasthi D, Kumar V, Chandra T, Kumar S, Dikshit M (2018) S-Glutathionylation of p47phox sustains superoxide generation in activated neutrophils. Biochim Biophys Acta Mol Cell Res 1865(2):444-454. doi: 10.1016/j.bbamcr.2017.11.014

Objective: To investigate the role of S-Glutathionylation of p47phox.

Summary: In Phorbol 12-myristate 13-acetate (PMA) or Nitric oxide (NO) treated neutrophils a subunit of NOX2, p47phox gets glutathionylated to sustain ROS generation along with a decrease in catalase, Grx-1 activity and change in GSH/GSSG ratio.

Usage: Western blot

Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)

PARK2 depletion connects energy and oxidative stress to PI3K/Akt activation via PTEN S-Nitrosylation

Gupta A, Anjomani-Virmouni S, Koundouros N, Dimitriadi M, Choo-Wing R, Valle A, Zheng Y, Chiu YH, Agnihotri S, Zadeh G, Asara JM, Anastasiou D, Arends MJ, Cantley LC, Poulogiannis G (2017) PARK2 depletion connects energy and oxidative stress to PI3K/Akt activation via PTEN S-Nitrosylation. Mol Cell 65(6):999-101.e7. doi: 10.1016/j.molcel.2017.02.019

Objective: To investigate the missing piece in the dynamic signaling and metabolic network governing PI3K/Akt activation.

Summary: PARK2 inactivation connects energy and oxidative stress to Akt activation via redox-mediated inactivation of PTEN by S-nitrosylation to support cell survival under conditions of energy deprivation.

Usage: Immunoblotting

Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)

Evidence for nitric oxide involvement in experimental autoimmune encephalomyelitis and adjuvant-induced arthritis in Lewis rat.

Mnaimneh S, Damaj M, Barhoumi R, Mouneimne Y, Geffard M, Veyret B, Vincendeau P (2004) Evidence for nitric oxide involvement in experimental autoimmune encephalomyelitis and adjuvant-induced arthritis in Lewis rat. The Pain Clinic 16(3):229-243. doi: 10.1163/1568569041798407

Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)

S-nitrosating nitric oxide donors induce long-lasting inhibition of contraction in isolated arteries.

Alencar JL, Lobysheva I, Chalupsky K, Geffard M, Nepveu F, Stoclet JC, Muller B (2003) S-nitrosating nitric oxide donors induce long-lasting inhibition of contraction in isolated arteries. J Pharmacol Exp Ther 307(1):152-159. doi: 10.1124/jpet.103.052605

Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)

Role of S-nitrosation of cysteine residues in long-lasting inhibitory effect of nitric oxide on arterial tone.

Alencar JL, Lobysheva I, Geffard M, Sarr M, Schott C, Schini-Kerth VB, Nepveu F, Stoclet JC, Muller B (2003) Role of S-nitrosation of cysteine residues in long-lasting inhibitory effect of nitric oxide on arterial tone. Mol Pharmacol 63(5):1148-1158. doi: 10.1124/mol.63.5.1148

Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)

Immunohistochemical localization of protein 3-nitrotyrosine and S-nitrosocysteine in a murine model of inhaled nitric oxide therapy.

Lorch SA, Foust R 3rd, Gow A, Arkovitz M, Salzman AL, Szabo C, Vayert B, Geffard M, Ischiropoulos H (2000) Immunohistochemical localization of protein 3-nitrotyrosine and S-nitrosocysteine in a murine model of inhaled nitric oxide therapy. Pediatr Res 47(6):798-805. doi: 10.1203/00006450-200006000-00020

Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)

Detection of nitrosylated epitopes in Trypanosoma brucei gambiense by polyclonal and monoclonal anti-conjugated-NO-cysteine antibodies.

Mnaimneh S, Geffard M, Veyret B, Vincendeau P (1999) Detection of nitrosylated epitopes in Trypanosoma brucei gambiense by polyclonal and monoclonal anti-conjugated-NO-cysteine antibodies. C R Acad Sci III 322(4):311-322. doi: 10.1016/s0764-4469(99)80067-5

Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)

Albumin nitrosylated by activated macrophages possesses antiparasitic effects neutralized by anti-NO-acetylated-cysteine antibodies.

Mnaimneh S, Geffard M, Veyret B, Vincendeau P (1997) Albumin nitrosylated by activated macrophages possesses antiparasitic effects neutralized by anti-NO-acetylated-cysteine antibodies. J Immunol 158(1):308-314.

Related Products: NO-L-Cysteine Mouse Monoclonal, Conjugated (Cat. #AB-T125)