Trans-hydroxyproline Rabbit Polyclonal References

Cat #AB-T044

Zheng F, Chen J, Zhang X, Wang Z, Chen J, Lin X, Huang H, Fu W, Liang J, Wu W, Li B, Yao H, Hu H, Song E. The HIF-1α antisense long non-coding RNA drives a positive feedback loop of HIF-1α mediated transactivation and glycolysis. (2021) Nat Commun 12(1):1341. doi: 10.1038/s41467-021-21535-3. Western blot (1:500).

Objective: To investigate the contributions of HIF-1 in regulating glycolysis of cancer cells under hypoxia
Summary: HIF Antisense LncRNA (HIFAL) has a critical regulatory role in HIF-1-driven transactivation and glycolysis, identifying HIFAL as a therapeutic target of cancer treatment.

Lee SB, Ko A, Oh YT, Shi P, D’Angelo F, Frangaj B, Koller A, Chen EI, Cardozo T, Iavarone A, Lasorella A. Proline Hydroxylation Primes Protein Kinases for Autophosphorylation and Activation (2020) Mol Cell 79(3):376-389.e8. doi: 10.1016/j.molcel.2020.06.021. Western Blot

Objective: To investigate the formation of prolyl-hydroxylated intermediates as a novel mechanism of kinase maturation and a general mechanism of regulation of CMGC kinases in eukaryotes.
Summary: Identified a highly conserved proline in the kinase domain of CMGC kinases that is hydroxylated by the PHD1 prolyl-hydroxylase, and this event precedes tyrosine autophosphorylation.

Ringel AE, Drijvers JM, Baker GJ, Catozzi A, García-Cañaveras JC, Gassaway BM, Miller BC, Juneja VR, Nguyen TH, Joshi S, Yao CH, Yoon H, Sage PT, LaFleur MW, Trombley JD, Jacobson CA, Maliga Z, Gygi SP, Sorger PK, Rabinowitz JD, Sharpe AH, Haigis MC. Obesity Shapes Metabolism in the Tumor Microenvironment to Suppress Anti-Tumor Immunity (2020) Cell 183(7):1848-1866.e26. doi: 10.1016/j.cell.2020.11.009.  Immunoprecipitation.

Objective: To investigate how obesity shifts the metabolic landscape of the tumor microenvironment (TME) to inhibit T cell function and promote tumor growth.
Summary: High-fat diet – induced obesity impairs CD8+ T cell function in the murine TME, accelerating tumor growth.

Zhao L, Wang J, Zhang Y, Wang L, Yu M, Wang F. Vitamin C decreases VEGF expression levels via hypoxia‑inducible factor‑1α dependent and independent pathways in lens epithelial cells. (2020) Mol Med Rep 22(1):436-444. doi: 10.3892/mmr.2020.11103. Western blot (1:500).

Objective: To investigate the mechanisms underlying the effects of vitamin C on the expression levels of VEGF.
Summary: Vitamin C inhibits the cell proliferation and the expression levels of VEGF in lens epithelial cells following a co-treatment with the HIF-1 inhibitor.

Zurlo G, Liu X, Takada M, Fan C, Simon JM, Ptacek TS, Rodriguez J, von Kriegsheim A, Liu J, Locasale JW, Robinson A, Zhang J, Holler JM, Kim B, Zikánová M, Bierau J, Xie L, Chen X, Li M, Perou CM, Zhang Q. Prolyl hydroxylase substrate adenylosuccinate lyase is an oncogenic driver in triple negative breast cancer (2019) Nat Commun 10(1):5177. doi: 10.1038/s41467-019-13168-4. Western blot.

Objective: To investigate the role of ADSL hydroxylation in controlling cMYC and triple negative breast cancer (TNBC) tumorigenesis.
Summary: ADSL regulates cMYC protein level through adenosine levels and leads to downstream metabolic changes and breast cancer cell proliferation. ADSL is essential for TNBC cell growth and invasiveness and contributes to TNBC breast tumorigenesis and metastasis in vivo.

Casciello F, Al-Ejeh F, Kelly G, Brennan D, Ngiow S, Young A, Stoll T, Windloch K, Hill M, Smyth M, Gannon F, Lee J. G9a drives hypoxia-mediated gene repression for breast cancer cell survival and tumorigenesis. (2017) Proc Natl Acad Sci U S A 114:7077-7082. doi: 10.1073/pnas.1618706114.  Western blot.

Ullah K, Rosendahl AH, Izzi V, Bergmann U, Pihlajaniemi T, Maki JM, Myllyharju JA-Ohoo. Hypoxia-inducible factor prolyl-4-hydroxylase-1 is a convergent point in the reciprocal negative regulation of NF-kappaB and p53 signaling pathways (2017) Sci Rep 7:172200. doi: 10.1038/s41598-017-17376-0.  Western blot.

Lee SB, Frattini V, Bansal M, Castano AM, Sherman D, Hutchinson K, Bruce JN, Califano A, Liu G, Cardozo T, Iavarone A, Lasorella A. An ID2-dependent mechanism for VHL inactivation in cancer. (2016) Nature 529(7585):172-177. doi: 10.1038/nature16475.  Western blot.

Luo W, Lin B, Wang Y, Zhong J, O’Meally R, Cole R, Pandey A, Levchenko A, Semenza G. PHD3-mediated prolyl hydroxylation of nonmuscle actin impairs polymerization and cell motility. (2014) Mol Biol Cell 25:2788-2796. doi: 10.1091/mbc.E14-02-0775. Western blot.

Zheng X, Zhai B, Koivunen P, Shin S, Lu G, Liu J, Geisen C, Chakraborty A, Moslehi J, Smalley D, Wei X, Chen X, Chen Z, Beres J, Zhang J, Tsao J, Brenner M, Zhang Y, Fan C, DePinho R, Paik J, Gygi S, Kaelin W, Zhang Q. Prolyl hydroxylation by EglN2 destabilizes FOXO3a by blocking its interaction with the USP9x deubiquitinase. (2014) Genes Dev 28:1429-1444. doi: 10.1101/gad.242131.114.  Western Blot.  (Targeting Trends 14q4)

Jung IH, Park JC, Kim JC, Jeon DW, Choi SH, Cho KS, Im GI, Kim BS, Kim CS. Novel application of human periodontal ligament stem cells and water-soluble chitin for collagen tissue regeneration: in vitro and in vivo investigations. (2012) Tissue Eng Part A 18(5-6):643-53. doi: 10.1089/ten.TEA.2011.0164.  Western blot 1:1000