Trans-4-Hydroxy-L-Proline Rabbit Polyclonal, Conjugated (Cat. #AB-T044)
12 entries found for : ab-t044
Li S, Li W, Yuan J, Bullova P, Wu J, Zhang X, Liu Y, Plescher M, Rodriguez J, Bedoya-Reina OC, Jannig PR, Valente-Silva P, Yu M, Henriksson MA, Zubarev RA, Smed-Sörensen A, Suzuki CK, Ruas JL, Holmberg J, Larsson C, Christofer Juhlin C, von Kriegsheim A, Cao Y, Schlisio S (2022) Impaired oxygen-sensitive regulation of mitochondrial biogenesis within the von Hippel-Lindau syndrome. Nat Metab 4(6):739-758. doi: 10.1038/s42255-022-00593-x PMID: 35760869
Objective: To investigate how mitochondria sense oxygen levels.
Summary: The authors report an oxygen-sensitive regulation of TFAM, an activator of mitochondrial transcription and replication, whose alteration is linked to tumours arising in the von Hippel–Lindau syndrome. The data obtained from this study offer pharmacological avenues to sensitize therapy-resistant VHL tumours by focusing on the mitochondria.
Usage: Immunoprecipitation (IP), Immunoblot (IB); 1:1000
Related Products: Trans-4-Hydroxy-L-Proline Rabbit Polyclonal, Conjugated (Cat. #AB-T044)
Zheng F, Chen J, Zhang X, Wang Z, Chen J, Lin X, Huang H, Fu W, Liang J, Wu W, Li B, Yao H, Hu H, Song E (2021) The HIF-1α antisense long non-coding RNA drives a positive feedback loop of HIF-1α mediated transactivation and glycolysis. Nat Commun 12(1):1341. doi: 10.1038/s41467-021-21535-3 PMID: 33637716
Objective: To investigate the contributions of HIF-1 in regulating glycolysis of cancer cells under hypoxia
Summary: HIF Antisense LncRNA (HIFAL) has a critical regulatory role in HIF-1-driven transactivation and glycolysis, identifying HIFAL as a therapeutic target of cancer treatment.
Usage: Western blot (1:500)
Related Products: Trans-4-Hydroxy-L-Proline Rabbit Polyclonal, Conjugated (Cat. #AB-T044)
Obesity shapes metabolism in the tumor microenvironment to suppress anti-tumor immunity
Ringel AE, Drijvers JM, Baker GJ, Catozzi A, García-Cañaveras JC, Gassaway BM, Miller BC, Juneja VR, Nguyen TH, Joshi S, Yao CH, Yoon H, Sage PT, LaFleur MW, Trombley JD, Jacobson CA, Maliga Z, Gygi SP, Sorger PK, Rabinowitz JD, Sharpe AH, Haigis MC (2020) Obesity shapes metabolism in the tumor microenvironment to suppress anti-tumor immunity. Cell 183(7):1848-1866.e26. doi: 10.1016/j.cell.2020.11.009 PMID: 33301708
Objective: To investigate how obesity shifts the metabolic landscape of the tumor microenvironment (TME) to inhibit T cell function and promote tumor growth.
Summary: High-fat diet – induced obesity impairs CD8+ T cell function in the murine TME, accelerating tumor growth.
Usage: Immunoprecipitation
Related Products: Trans-4-Hydroxy-L-Proline Rabbit Polyclonal, Conjugated (Cat. #AB-T044)
Proline hydroxylation primes protein kinases for autophosphorylation and activation
Lee SB, Ko A, Oh YT, Shi P, D'Angelo F, Frangaj B, Koller A, Chen EI, Cardozo T, Iavarone A, Lasorella A (2020) Proline hydroxylation primes protein kinases for autophosphorylation and activation. Mol Cell 79(3):376-389.e8. doi: 10.1016/j.molcel.2020.06.021 PMID: 32640193
Objective: To investigate the formation of prolyl-hydroxylated intermediates as a novel mechanism of kinase maturation and a general mechanism of regulation of CMGC kinases in eukaryotes.
Summary: Identified a highly conserved proline in the kinase domain of CMGC kinases that is hydroxylated by the PHD1 prolyl-hydroxylase, and this event precedes tyrosine autophosphorylation.
Usage: Western blot
Related Products: Trans-4-Hydroxy-L-Proline Rabbit Polyclonal, Conjugated (Cat. #AB-T044)
Zhao L, Wang J, Zhang Y, Wang L, Yu M, Wang F (2020) Vitamin C decreases VEGF expression levels via hypoxia‑inducible factor‑1α dependent and independent pathways in lens epithelial cells. Mol Med Rep 22(1):436-444. doi: 10.3892/mmr.2020.11103 PMID: 32377733
Objective: To investigate the mechanisms underlying the effects of vitamin C on the expression levels of VEGF.
Summary: Vitamin C inhibits the cell proliferation and the expression levels of VEGF in lens epithelial cells following a co-treatment with the HIF-1 inhibitor.
Usage: Western blot (1:500)
Related Products: Trans-4-Hydroxy-L-Proline Rabbit Polyclonal, Conjugated (Cat. #AB-T044)
Zurlo G, Liu X, Takada M, Fan C, Simon JM, Ptacek TS, Rodriguez J, von Kriegsheim A, Liu J, Locasale JW, Robinson A, Zhang J, Holler JM, Kim B, Zikánová M, Bierau J, Xie L, Chen X, Li M, Perou CM, Zhang Q (2019) Prolyl hydroxylase substrate adenylosuccinate lyase is an oncogenic driver in triple negative breast cancer. Nat Commun 10(1):5177. doi: 10.1038/s41467-019-13168-4 PMID: 31729379
Objective: To investigate the role of ADSL hydroxylation in controlling cMYC and triple negative breast cancer (TNBC) tumorigenesis.
Summary: ADSL regulates cMYC protein level through adenosine levels and leads to downstream metabolic changes and breast cancer cell proliferation. ADSL is essential for TNBC cell growth and invasiveness and contributes to TNBC breast tumorigenesis and metastasis in vivo.
Usage: Western blot
Related Products: Trans-4-Hydroxy-L-Proline Rabbit Polyclonal, Conjugated (Cat. #AB-T044)
Ullah K, Rosendahl AH, Izzi V, Bergmann U, Pihlajaniemi T, Maki JM, Myllyharju JA-Ohoo (2017) Hypoxia-inducible factor prolyl-4-hydroxylase-1 is a convergent point in the reciprocal negative regulation of NF-κB and p53 signaling pathways. Sci Rep 7:172200. doi: 10.1038/s41598-017-17376-0 PMID: 29222481
Usage: Western blot
Related Products: Trans-4-Hydroxy-L-Proline Rabbit Polyclonal, Conjugated (Cat. #AB-T044)
G9a drives hypoxia-mediated gene repression for breast cancer cell survival and tumorigenesis.
Casciello F, Al-Ejeh F, Kelly G, Brennan D, Ngiow S, Young A, Stoll T, Windloch K, Hill M, Smyth M, Gannon F, Lee J (2017) G9a drives hypoxia-mediated gene repression for breast cancer cell survival and tumorigenesis. Proc Natl Acad Sci U S A 114:7077-7082. doi: 10.1073/pnas.1618706114 PMID: 28630300
Usage: Western blot
Related Products: Trans-4-Hydroxy-L-Proline Rabbit Polyclonal, Conjugated (Cat. #AB-T044)
An ID2-dependent mechanism for VHL inactivation in cancer.
Lee SB, Frattini V, Bansal M, Castano AM, Sherman D, Hutchinson K, Bruce JN, Califano A, Liu G, Cardozo T, Iavarone A, Lasorella A (2016) An ID2-dependent mechanism for VHL inactivation in cancer. Nature 529(7585):172-177. doi: 10.1038/nature16475 PMID: 26735018
Usage: Western blot
Related Products: Trans-4-Hydroxy-L-Proline Rabbit Polyclonal, Conjugated (Cat. #AB-T044)
PHD3-mediated prolyl hydroxylation of nonmuscle actin impairs polymerization and cell motility.
Luo W, Lin B, Wang Y, Zhong J, O'Meally R, Cole R, Pandey A, Levchenko A, Semenza G (2014) PHD3-mediated prolyl hydroxylation of nonmuscle actin impairs polymerization and cell motility. Mol Biol Cell 25:2788-2796. doi: 10.1091/mbc.E14-02-0775 PMID: 25079693
Usage: Western blot
Related Products: Trans-4-Hydroxy-L-Proline Rabbit Polyclonal, Conjugated (Cat. #AB-T044)
Zheng X, Zhai B, Koivunen P, Shin S, Lu G, Liu J, Geisen C, Chakraborty A, Moslehi J, Smalley D, Wei X, Chen X, Chen Z, Beres J, Zhang J, Tsao J, Brenner M, Zhang Y, Fan C, DePinho R, Paik J, Gygi S, Kaelin W, Zhang Q (2014) Prolyl hydroxylation by EglN2 destabilizes FOXO3a by blocking its interaction with the USP9x deubiquitinase. Genes Dev 28:1429-1444. doi: 10.1101/gad.242131.114 PMID: 24990963
Summary: Members of the FOXO family are thought to act as tumor suppressor genes. In this work the authors investigated the hydroxylation of FOXO3a by EglN2. This hydroxylation pushes FOXO3a toward a protosomal degradation pathway. Loss of FOXO3a in turn allows the accumulation of Cyclin D1, which has been found to be overexpressed in some breast cancers. Some of the data were generated using immunoblots with anti-transhydroxylated proline (Cat. #AB-T044).
Usage: Western blot
Related Products: Trans-4-Hydroxy-L-Proline Rabbit Polyclonal, Conjugated (Cat. #AB-T044)
Jung IH, Park JC, Kim JC, Jeon DW, Choi SH, Cho KS, Im GI, Kim BS, Kim CS (2012) Novel application of human periodontal ligament stem cells and water-soluble chitin for collagen tissue regeneration: in vitro and in vivo investigations. Tissue Eng Part A 18(5-6):643-53. doi: 10.1089/ten.TEA.2011.0164 PMID: 21981356
Usage: Western blot 1:1000
Related Products: Trans-4-Hydroxy-L-Proline Rabbit Polyclonal, Conjugated (Cat. #AB-T044)