Trans-hydroxyproline Rabbit Polyclonal References

Cat #AB-T044

Zheng F, Chen J, Zhang X, Wang Z, Chen J, Lin X…Song E. The HIF-1α antisense long non-coding RNA drives a positive feedback loop of hif-1α mediated transactivation and glycolysis. (2021). Nature communications, 12 (1):1341. 10.1038/s41467-021-21535-3. Western blot (1:500).

Objective: To investigate the contributions of HIF-1 in regulating glycolysis of cancer cells under hypoxia
Summary: HIF Antisense LncRNA (HIFAL) has a critical regulatory role in HIF-1-driven transactivation and glycolysis, identifying HIFAL as a therapeutic target of cancer treatment.

Lee SB, Ko A, Oh YT, Shi P, D’Angelo F, Frangaj B…Lasorella A. Proline hydroxylation primes protein kinases for autophosphorylation and activation. (2020). Mol Cell, 79 (3):376-389.e378. 2020/07/09. PMC7849370 10.1016/j.molcel.2020.06.021. Western Blot (1:500)

Objective: To investigate the contributions of HIF-1 in regulating glycolysis of cancer cells under hypoxia.
Summary: HIF Antisense LncRNA (HIFAL) has a critical regulatory role in HIF-1-driven transactivation and glycolysis, identifying HIFAL as a therapeutic target of cancer treatment.

Ringel AE, Drijvers JM, Baker GJ, Catozzi A, García-Cañaveras JC, Gassaway BM…Haigis MC. Obesity shapes metabolism in the tumor microenvironment to suppress anti-tumor immunity. (2020). Cell, 183 (7):1848-1866.e1826. 10.1016/j.cell.2020.11.009.  Immunoprecipitation.

Objective: To investigate how obesity shifts the metabolic landscape of the tumor microenvironment (TME) to inhibit T cell function and promote tumor growth.
Summary: High-fat diet – induced obesity impairs CD8+ T cell function in the murine TME, accelerating tumor growth.

Zhao L, Wang J, Zhang Y, Wang L, Yu M, & Wang F. Vitamin c decreases VEGF expression levels via hypoxia‑inducible factor‑1α dependent and independent pathways in lens epithelial cells. (2020). Mol Med Rep, 22 (1):436-444. 10.3892/mmr.2020.11103. Western blot (1:500).

Objective: To investigate the mechanisms underlying the effects of vitamin C on the expression levels of VEGF.
Summary: Vitamin C inhibits the cell proliferation and the expression levels of VEGF in lens epithelial cells following a co-treatment with the HIF-1 inhibitor.

Zurlo G, Liu X, Takada M, Fan C, Simon JM, Ptacek TS…Zhang Q. Prolyl hydroxylase substrate adenylosuccinate lyase is an oncogenic driver in triple negative breast cancer. (2019). Nature communications, 10 (1):5177. 10.1038/s41467-019-13168-4. Western blot.

Objective: To investigate the role of ADSL hydroxylation in controlling cMYC and triple negative breast cancer (TNBC) tumorigenesis.
Summary: ADSL regulates cMYC protein level through adenosine levels and leads to downstream metabolic changes and breast cancer cell proliferation. ADSL is essential for TNBC cell growth and invasiveness and contributes to TNBC breast tumorigenesis and metastasis in vivo.

Ullah K, Rosendahl AH, Izzi V, Bergmann U, Pihlajaniemi T, Maki JM, & Myllyharju JA-Ohoo. Hypoxia-inducible factor prolyl-4-hydroxylase-1 is a convergent point in the reciprocal negative regulation of NF-kappaB and p53 signaling pathways. (2017). Sci Rep, 7 (1):172200.  Western blot.

Casciello F, Al-Ejeh F, Kelly G, Brennan DJ, Ngiow SF, Young A, Stoll T, Windloch K, Hill MM, Smyth MJ, Gannon F, & Lee JS. G9a drives hypoxia-mediated gene repression for breast cancer cell survival and tumorigenesis. (2017). Proc Natl Acad Sci, 114 (27):7077.  Western blot.

Lee SB, Frattini V, Bansal M, Castano AM, Sherman D, Hutchinson K, Bruce JN, Califano A, Liu G, Cardozo T, Iavarone A, & Lasorella A. An ID2-dependent mechanism for VHL inactivation in cancer. (2016). Nature, 529 (7585):172-177. 2016/01/07. PMC5384647.  Western blot.

Luo W, Lin B, Wang Y, Zhong J, O’Meally R, Cole RN, Pandey A, Levchenko A, & Semenza GL. Phd3-Mediated Prolyl Hydroxylation of Nonmuscle Actin Impairs Polymerization and Cell Motility. (2014). Mol Biol Cell, 25 (18):2788-2796. 2014/08/01. Pmc4161513.
Western blot.

Zheng X, Zhai B, Koivunen P, Shin SJ, Lu G, Liu J, Geisen C, Chakraborty AA, Moslehi JJ, Smalley DM, Wei X, Chen X, Chen Z, Beres JM, Zhang J, Tsao JL, Brenner MC, Zhang Y, Fan C, DePinho RA, Paik J, Gygi SP, Kaelin WGJ, Zhang Q. (2014) Prolyl hydroxylation by EglN2 destabilizes FOXO3a by blocking its interaction with the USP9x deubiquitinase. Genes Dev 28(13):1429-1444.  Western Blot.  (Targeting Trends 14q4)

Jung IH, Park JC, Kim JC, Jeon DW, Choi SH, Cho KS, Im GI, Kim BS, & Kim CS. Novel Application of Human Periodontal Ligament Stem Cells and Water-Soluble Chitin for Collagen Tissue Regeneration: In Vitro and In Vivo Investigations. (2011). Tissue Engineer Part A, 18 (5-6):643-653.  Western blot 1:1000