vGAT Rabbit Polyclonal References

Cat. #AB-N44; AB-N44AP

Friedman CA, Russell BJ, Kohls MD, Ancheta LR, Shramm PA, Lappi DA (2016) Targeting vesicular gaba transporter (vGAT)-expressing cells with a polyclonal antibody to the lumenal domain of vGAT: results with a saporin conjugate. Soc Neurosci Meeting Abstract 124.06

Bitzenhofer SH, Hanganu-Opatz IL (2014) Oscillatory coupling within neonatal prefrontal-hippocampal networks is independent of selective removal of GABAergic neurons in the hippocampus. Neuropharmacology 77:57-67. doi: 10.1016/j.neuropharm.2013.09.007

Summary: During cognitive tasks neuronal networks are entrained by oscillatory electrical rhythms with different frequencies. It has been proposed that GABAergic neurons in the prefrontal-hippocampal networks control this processing. The authors administered 252 ng of Anti-vGAT-SAP (Cat. #IT-71) into the ventral hippocampus of rats to examine how the GABAergic neurons could be involved. Unconjugated anti-vGAT (Cat #AB-N44) was used as a control. Hippocampal sharp waves were impaired during neonatal development, but the data indicate that oscillatory coupling between the neonatal prefrontal cortex and hippocampus is not controlled by GABAergic hippocampal interneurons.

Andäng M, Hjerling-Leffler J, Moliner A, Lundgren TK, Castelo-Branco G, Nanou E, Pozas E, Bryja V, Halliez S, Nishimaru H, Wilbertz J, Arenas E, Koltzenburg M, Charnay P, Manira AE, Ibañez CF, Ernfors P (2008) Histone H2AX-dependent GABAA receptor regulation of stem cell proliferation. Nature 451(7177):460-464. doi: 10.1038/nature06488

Summary: Immunofluorescence staining for vesicular GABA transporter (VGAT) in embryonic stem cells reveals a punctate localization.
Dose: Immunostaining and western blotting.  After being blocked for 2 h in PBS containing 3% BSA and 0.3% Triton X-100 and after two washes in PBS, cells were incubated in primary antibody against VGAT (diluted 1:400).

Gertow K, Wolbank S, Rozell B, Sugars R, Andang M, Parish CL, Imreh MP, Wendel M, Ahrlund-Richter L (2004) Organized development from human embryonic stem cells after injection into immunodeficient mice. Stem Cells Dev 13:421-435. doi: 10.1089/scd.2004.13.421

Summary: After thawing and brief drying, sections were fixed with 4% paraformaldehyde in PBS pH 7.4 for 40 min.  Sections were blocked [PBS, 1% bovine serum albumin (BSA), 0.3% Triton X-100], washed, and incubated with primary antibodies for Tuj1 (-tubulin type III), glial fibrillary acid protein (GFAP), tyrosine hydroxylase (TH), and vesicular GABA transporter (vGAT; 1:400) overnight at 4°C.  After rinsing in PBS, slides were incubated with Cy2- or Cy3-conjugated secondary antibodies.