Thal LB, Kovtun O, & Rosenthal SJ. (2020). Labeling Neuronal Proteins with Quantum Dots for Single-Molecule Imaging. In A Fontes & B Santos (Eds.), Quantum Dots. Methods in Molecular Biology (Vol. 2135). New York, NY: Humana.
Summary: The authors describe how nanometer-sized fluorescent semiconductors called quantum dots (QD) can be used to label neuronal proteins in a single QD imaging format.
Dose: Secondary antibody-conjugated QDs target membrane proteins pre-treated with SERT Mouse Monoclonal.
Bailey DM, Catron MA, Kovtun O, Macdonald RL, Zhang Q, & Rosenthal SJ. Single Quantum Dot Tracking Reveals Serotonin Transporter Diffusion Dynamics Are Correlated with Cholesterol-Sensitive Threonine 276 Phosphorylation Status in Primary Midbrain Neurons. (2018). ACS Chem Neurosci 10.1021/acschemneuro.8b00214
Objective: Visualizing SERT behavior at the single molecule level in endogenous systems remains a challenge. The authors utilize quantum dot (QD) single particle tracking (SPT) to capture SERT dynamics in primary rat midbrain neurons.
Summary: Results provide new insights into endogenous neuronal SERT mobility and its associations with membrane cholesterol and SERT phosphorylation status.
Dose: Live Cell QD Tracking – Cells were incubated with 5 μg/mL primary SERT antibody in warm fluorescent media at 37 °C and 5% CO2 for 6 min.
Fraser M, Fortier M, Foucher D, Roumier PH, Brousseau P, Fournier M, Surette C, & Vaillancourt C. Exposure to Low Environmental Concentrations of Manganese, Lead, and Cadmium Alters the Serotonin System of Blue Mussels. (2017). Environmental Toxicology and Chemistry, 37 (1):192-200.
Objective: To determine the effects of exposure to Mn, Pb, or Cd on serotonin levels, monoamine oxidase (MAO) activity, and serotonin transporter (SERT) levels in the blue mussel Mytilus edulis.
Summary: The results clearly indicate that low environmental concentrations of Mn, Pb, and Cd affect the serotonin system in blue mussels.
Dose: Membrane was exposed to SERT primary antibody diluted 1:1000 in 5% PBS skimmed milk for 1.5 h at room temperature, then rinsed twice in deionized water, washed 3 times for 5 min in PBS, and rinsed 4 times in deionized water.
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