Bailey DM, Catron MA, Kovtun O, Macdonald RL, Zhang Q, & Rosenthal SJ. Single Quantum Dot Tracking Reveals Serotonin Transporter Diffusion Dynamics Are Correlated with Cholesterol-Sensitive Threonine 276 Phosphorylation Status in Primary Midbrain Neurons. (2018). ACS Chem Neurosci 10.1021/acschemneuro.8b00214
Objective: Visualizing SERT behavior at the single molecule level in endogenous systems remains a challenge. The authors utilize quantum dot (QD) single particle tracking (SPT) to capture SERT dynamics in primary rat midbrain neurons.
Summary: Results provide new insights into endogenous neuronal SERT mobility and its associations with membrane cholesterol and SERT phosphorylation status.
Dose: Live Cell QD Tracking – Cells were incubated with 5 μg/mL primary SERT antibody in warm fluorescent media at 37 °C and 5% CO2 for 6 min.
Fraser M, Fortier M, Foucher D, Roumier PH, Brousseau P, Fournier M, Surette C, & Vaillancourt C. Exposure to Low Environmental Concentrations of Manganese, Lead, and Cadmium Alters the Serotonin System of Blue Mussels. (2017). Environmental Toxicology and Chemistry, 37 (1):192-200.
Objective: To determine the effects of exposure to Mn, Pb, or Cd on serotonin levels, monoamine oxidase (MAO) activity, and serotonin transporter (SERT) levels in the blue mussel Mytilus edulis.
Summary: The results clearly indicate that low environmental concentrations of Mn, Pb, and Cd affect the serotonin system in blue mussels.
Dose: Membrane was exposed to SERT primary antibody diluted 1:1000 in 5% PBS skimmed milk for 1.5 h at room temperature, then rinsed twice in deionized water, washed 3 times for 5 min in PBS, and rinsed 4 times in deionized water.
Steiner JA, Carneiro AM, Wright J, Matthies HJ, Prasad HC, Nicki CK, Dostmann WR, Buchanan CC, Corbin JD, Francis SH, Blakely RD. (2009) cGMP-dependent protein kinase Ialpha associates with the antidepressant-sensitive serotonin transporter and dictates rapid modulation of serotonin uptake. Mol Brain 2:26. (Targeting Trends 14q4)
Lacerda CM, Kisiday J, Johnson B, Orton EC. (2012) Local serotonin mediates cyclic strain-induced phenotype transformation, matrix degradation, and glycosaminoglycan synthesis in cultured sheep mitral valves. Am J Physiol Heart Circ Physiol 302(10):H1983-90. (Targeting Trends 12q4)
Kirillova V, Prosviryakov E. (2012) Disrupted serotonergic system in patients with pulmonary hypertension may serve as novel biomarkers new therapeutic targets and to assess severity, progression and response to treatment. Cardiovasc Res 93 Suppl 1:P209. (Targeting Trends 12q3)
Marshall AM, Nommsen-Rivers LA, Hernandez LL, Dewey KG, Chantry CJ, Gregerson KA, Horseman ND (2010) Serotonin Transport and Metabolism in the Mammary Gland Modulates Secretory Activation and Involution. J Clin Endocrinol Metab 95(2):837-846. (Targeting Trends 10q2)
Beaulieu JM, Zhang X, Rodriguiz RM, Sotnikova TD, Cools MJ, Wetsel WC, Gainetdinov RR, Caron MG (2008) Role of GSK3 beta in behavioral abnormalities induced by serotonin deficiency. Proc Natl Acad Sci U S A 105(4):1333-1338.
Lau T, Horschitz S, Berger S, Bartsch D, Schloss P (2008) Antidepressant-induced internalization of the serotonin transporter in serotonergic neurons. FASEB J 22(6):1702-1714.
Muller HK, Wiborg O, Haase J (2006) Subcellular redistribution of the serotonin transporter by secretory carrier membrane protein 2. J Biol Chem 281(39):28901-28909.
O’Connell PJ, Wang X, Leon-Ponte M, Griffiths C, Pingle SC, Ahern GP (2006) A novel form of immune signaling revealed by transmission of the inflammatory mediator serotonin between dendritic cells and T cells. Blood 107(3):1010-1017.
Nattie EE, Li A, Richerson GB, Lappi DA (2004) Medullary serotonergic neurons and adjacent neurons that express neurokinin-1 receptors are both involved in chemoreception in vivo. J Physiol 556(1):235-253.