Angiotensin II receptor References

Cat #AB-N25AP, Cat #AB-N26AP, Cat #AB-N27AP, Cat #AB-N28AP


Gonzalez EA, Leitão SAT, Dos Santos Soares D, Tavares AMV, Giugliani R, Baldo G, & Matte U. Cardiac pathology in Mucopolysaccharidosis I mice: Losartan modifies ERK1/2 activation during cardiac remodeling. (2020). J Inherit Metab Dis (2020) 5 Nov. 10.1002/jimd.12327

AB-N27AP: Anti-Angiotensin Receptor (AT1R)
Objective:  To investigate whether the pathways influenced by losartan and propranolol may modulate the cardiac remodeling process in MPS I mice.
Summary: Losartan and propranolol restore the heart function and could be used to ameliorate the cardiac disease in MPS I.
Dose: AB-N27AP was used in Western Blot at 1:400

Lim I, Mitsui R, Kameda M, Sellers DJ, Chess-Williams R, & Hashitani H. Comparative effects of angiotensin II on the contractility of muscularis mucosae and detrusor in the pig urinary bladder. (2020). Neurourol Urodyn, online ahead of print (n/):

AB-N28AP:  Angiotensin II receptor antibody (AT2R)
Objective: To explore contractile actions of angiotensin II (AT2) on the muscularis mucosae (MM) of the bladder.
Summary: The MM appears to have great sensitivity to AT2, suggesting that MM may be the predominant target of contractile actions induced by AT2 in the bladder.
Dose: Induction of contractions with Anti-AT2R (1nM – 1uM). Immunofluorescence (10-100pM).

Bragina ME, Costa-Fraga F, Sturny M, et al. Characterization of the Renin-Angiotensin System in Aged Cavernosal Tissue and its Role in Penile Fibrosis.  (2020). J Sex Med
AB-N27AP: Anti-Angiotensin (AT1)
Application:  immunohistochemistry (1:800)

Kim M, Do GY, & Kim I. Activation of the renin-angiotensin system in high fructose-induced metabolic syndrome. (2020). Korean J Physiol & Pharmacol 24(4):319-328.   10.4196/kjpp.2020.24.4.319
AB-N27AP: Anti-Angiotensin (AT1)
Application: immunohistochemistry


Beitia M, Solano-Iturri JD, Errarte P, Sanz B, Perez I, Etxezarraga MC, Loizate A, Asumendi A, & Larrinaga G. Altered Expression of Renin-Angiotensin System Receptors Throughout Colorectal Adenoma-Adenocarcinoma Sequence. (2019). Int J Med Sci, 16 (6):813-821.
Application: immunohistochemistry (1:100)

Zhou G, Li J, Zeng T, Yang P, & Li A. The Regulation Effect of Wnt-Ras Signaling in Hypothalamic Paraventricular Nucleus on Renal Fibrosis. (2019). J Nephrol,
Application:  immunohistochemistry and western blot

Zhang Z, Jiang SM, Ma YP, Dai PL, Wang YN, Zou GM, Gao HM, Yang Y, & Li WG.  Expression of the intrarenal angiotensin receptor and the role of renin-angiotensin system inhibitors in IgA nephropathy. (2019). Mol Cell Biochem, 453 (1-2):103-110. 2018/08/31.
AB-N28AP:  Angiotensin IIR (AT2R)
Application:  immunohistochemistry (1:500)

Abd El-Rahman SS, & Fayed HM. Targeting AngII/AT1R signaling pathway by perindopril inhibits ongoing liver fibrosis in rat. (2019). J Tissue Eng Regen Med Jul 26. doi: 10.1002/term.2940. [Epub ahead of print]
AB-N28AP:  Angiotensin IIR (AT2R)
Application:  immunohistochemistry (1:100)

Aoyagi Y, Furuyama T, Inoue K, Matsuda D, Matsubara Y, Okahara A, Ago T, Nakashima Y, Mori M, & Matsumoto T. Attenuation of Angiotensin II–Induced Hypertension in BubR1 Low‐Expression Mice Via Repression of Angiotensin II Receptor 1 Overexpression. (2019). J Amer Heart Assoc, 8 (23):e011911.
AB-N27AP:  Angiotensin IIR (AT1R)
     Application: Western

Bhat SA, Sood A, Shukla R, & Hanif K. AT2R Activation Prevents Microglia Pro-inflammatory Activation in a NOX-Dependent Manner: Inhibition of PKC Activation and p47(phox) Phosphorylation by PP2A. (2019). Mol Neurobiol, 56 (4):3005-3023.
AB-N27AP:  Angiotensin IIR (AT1R) and AB-N28AP:  Angiotensin IIR (AT2R)

Objective: To investigate the involvement of AT2R in NADPH oxidase (NOX)-mediated microglia activation.
Summary: AT2R, via PP2A-mediated inhibition of PKC, prevents the NOX activation, ROS generation, and subsequent pro-inflammatory activation of microglia.
Dose:  Immunoblotting: membranes were incubated with respective primary antibodies AT1R (1:1000), AT2R (1:500). Immunocytochemistry: AT1R (1:100),
AT2R (1:100).

Ortgies DH, García-Villalón ÁL, Granado M, Amor S, Rodríguez EM, Santos HDA, Yao J, Rubio-Retama J, & Jaque D. Infrared fluorescence imaging of infarcted hearts with Ag2S nanodots. (2019). Nano Res, 12 (4):749-757.
AB-N27AP:  Angiotensin IIR (AT1R)

Objective: To investigate the potential application of functionalized Ag2S nanodots for accurate imaging of damaged myocardium tissues after a myocardial infarction induced by either partial or global ischemia.
Summary: NIR-II images generated by ATII-functionalized Ag2S nanodots contain valuable information about the location and extension of damaged tissue in the myocardium allowing for a proper identification of the occluded artery as well as an indirect evaluation of the damage level.
Dose: Assessment of AT1R protein content in ischemic and non-ischemic hearts by Western blot (1:1000).

Suwannasual U, Lucero J, Davis G, McDonald JD, & Lund AK. Mixed Vehicle Emissions Induces Angiotensin II and Cerebral Microvascular Angiotensin Receptor Expression in C57Bl/6 Mice and Promotes Alterations in Integrity in a Blood-Brain Barrier Coculture Model. (2019). Toxicological Sciences, 170 (2):525-535.  AB-N27AP:  Angiotensin IIR (AT1R)

Objective: To investigate the hypothesis that inhalation exposure to a mixture of gasoline and diesel vehicle engine emissions (MVE) results in altered central nervous system microvascular integrity mediated by Ang II-AT1 signaling.
Summary: Inhalation exposure to traffic-generated pollutants results in altered BBB integrity, mediated through Ang II-AT1 signaling and inflammation, which is exacerbated by a high-fat diet.
Dose: Antibody was used in immunofluorescent staining of cerebral tissue. Brain sections (10 mm) were prepared for AT1 receptor (1:1000).

Soares DdS, Pinto GH, Lopes A, Caetano DSL, Nascimento TG, Andrades ME, Clausell N, Rohde LEP, Leitão SAT, & Biolo A. Cardiac hypertrophy in mice submitted to a swimming protocol: influence of training volume and intensity on myocardial renin-angiotensin system. (2019). Am J Physiol Regul Integr Comp Physiol, 316 (6):R776-R782. PMID: 31042418
AB-N27AP:  Angiotensin IIR (AT1R)

Objective: To analyze the cardiac hypertrophy phenotype and the classical and alternative RAS axes in the myocardium of mice submitted to swimming exercises of varying volume and intensity for the development of cardiac hypertrophy.
Summary: Increasing exercise volume and intensity did not induce a pathological hypertrophy phenotype, but instead improved the oxidative capacity, and this process might have the participation of the RAS alternative axis.
Dose: incubation at 4°C with anti-AT1R diluted in TTBS buffer with 5% non-fat 220 milk 1:800).

Xu X, Pan J, Li X, Cui Y, Mao Z, Wu B, Xu H, Zhou W, & Liu Y. Inhibition of Methamphetamine Self-Administration and Reinstatement by Central Blockade of Angiotensin II Receptor in Rats. (2019). J Pharmacol ExpTher, 369 (2):244. PMID: 30867225    AB-N27AP:  Angiotensin IIR (AT1R)

Objective: To explore the role of central angiotensin II receptor (ATR) in drug taking and seeking behavior associated with methamphetamine (METH) use disorder.
Summary: The AT1R-PLCβ-CREB signaling pathway was found to be associated with the effect of AT1R on the drug-taking and drug-seeking behavior involving METH use disorder.
Dose: Membranes were incubated at 4°C overnight (1:1000).

Abbasloo E, Najafipour H, & Vakili A. Chronic treatment with apelin, losartan and their combination reduces myocardial infarct size and improves cardiac mechanical function. (2019). Clin Exp Pharmacol Physiol, epub. AB-N27AP:  Angiotensin IIR (AT1R)

Objective:  To determine the effect of chronic pretreatment with apelin, losartan and their combination on ischemia-reperfusion (IR) injury in the isolated perfused rat heart and on the expression of apelin-13 receptor (APJ) and angiotensin type 1 receptor (AT1R) in the myocardium.
Summary:  Chronic pretreatment with apelin along with AT1R antagonist had more protective effects against IR injury.
Dose:  1:10000

Zhou G, Li J, Zeng T, Yang P, & Li A. The regulation effect of WNT-RAS signaling in hypothalamic paraventricular nucleus on renal fibrosis. (2019). J Nephrol, [Epub ahead of print]
AB-N27AP:  Angiotensin IIR (AT1R)

Dose:  Histology and IHC, Western blot.

Zhuo X, Wu Y, Yang Y, Gao L, Qiao X, & Chen T. LncRNA AK094457 promotes AngII-mediated hypertension and endothelial dysfunction through suppressing of activation of PPARγ. (2019). Life Sciences, 233 116745.
AB-N27AP:  Angiotensin IIR (AT1R)

Dose:  IHC

Ji J, Tao P, & He L. Kangxianling decoction prevents renal fibrosis in rats with 5/6 nephrectomy and inhibits Ang II-induced ECM production in glomerular mesangial cells. (2019). J Pharmacol Sci, 139 (4):367-372.
AB-N27AP:  Angiotensin IIR (AT1R)

Objective: To investigate effects of Kangxianling decoction on renal fibrosis in a rat model of 5/6 Nephrectomy (5/6 N) in vivo and in angiotensin II (Ang II)-treated rat glomerular mesangial cells (HBZY-1) in vitro.
Summary:  Kangxianling Decoction could reduce renal fibrosis, accompanied by inhibiting the production of ECM proteins and JNK, along with downregulation of TGF-β, Ang II.
Dose:  Western blot analysis (1:100).

Kim E, Hwang S-H, Kim H-K, Abdi S, & Kim HK. Losartan, an Angiotensin II Type 1 Receptor Antagonist, Alleviates Mechanical Hyperalgesia in a Rat Model of Chemotherapy-Induced Neuropathic Pain by Inhibiting Inflammatory Cytokines in the Dorsal Root Ganglia. (2019). Molec Neurobiol 1-12. 10.1007/s12035-019-1616-0
AB-N27AP:  Angiotensin IIR (AT1R)

Objective:  To assess losartan’s analgesic effect on paclitaxel-induced neuropathic pain (PINP) in rats and its mechanism of action in dorsal root ganglion (DRG).
Summary:  The mechanical thresholds, protein levels of inflammatory cytokines, and cellular location of AT1R and interleukin 1β (IL-1β) in the DRG were assessed with behavioral testing, Western blotting, and immunohistochemistry, respectively.
Dose:  Western blot (1:100).

Im E-J, Lee C-H, Moon P-G, Rangaswamy GG, Lee B, Lee JM, Lee J-C, Jee J-G, Bae J-S, Kwon T-K, Kang K-W, Jeong M-S, Lee J-E, Jung H-S, Ro H-J, Jun S, Kang W, Seo S-Y, Cho Y-E, Song B-J, & Baek M-C. Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A. (2019). Nature Commun, 10 (1):1387.
AB-N27AP:  Angiotensin IIR (AT1R)

Objective:  To identify a direct protein target of SFX in cancer cells.
Summary:  Using data from the BindingDB, SEA identified four proteins as target candidates, which are: endothelin receptor type A, kynurenine 3-monooxygenase, carbonic anhydrase 13, and angiotensin II type 1a receptor.
Dose:  Western blot (1:1000).


Cops J, et al. Selective abdominal venous congestion induces adverse renal and hepatic morphological and functional alterations despite a preserved cardiac function (2018) Sci Rep 8: (1):17757. AB-N27AP:  Angiotensin IIR (AT1R)

Objective: The consequences of isolated abdominal venous congestion on morphology and function of the kidneys, liver and heart were studied in a rat model.
Summary: This study provides relevant insight in the pathophysiology of abdominal congestion on organ function.
Dose:  PVDF membrane was incubated overnight at 4 °C in the presence of antibody (1/2000).

Zhou H, et al. Erythropoietin alleviates post-resuscitation myocardial dysfunction in rats potentially through increasing the expression of angiotensin II receptor type 2 in myocardial tissues (2018) Mol Med Rep 17: (4):5184-5192.
AB-N27AP:  Angiotensin IIR (AT1R)

Objective: To determine whether erythropoietin (EPO) improves post‑resuscitation myocardial dysfunction and how it affects the renin‑angiotensin system.
Summary:  The present study confirmed that EPO treatment is beneficial for protecting cardiac function post‑resuscitation, and the roles of EPO in alleviating post‑resuscitation myocardial dysfunction may potentially be associated with enhanced myocardial expression of AT2R.
Dose: Western blot analysis of AT1R (1:500) in the myocardium.

Wang C, et al. Angiotensin II induces monocyte chemoattractant protein1 expression by increasing reactive oxygen species mediated activation of the nuclear factor kappaB signaling pathway in osteoblasts (2018) Mol Med Rep 17: (1):1166-1172.
AB-N27AP Angiotensin IIR (AT1R)

Objective: To investigate the effect of angiotensin II (Ang II) on monocyte chemoattractant protein‑1 (MCP‑1) expression and the underlying mechanism in osteoblasts.
Summary: These data indicate that Ang II-enhanced ROS production and activated NF‑κB signaling via AT1R, thus upregulating MCP‑1 expression in osteoblasts.
Dose: The membranes were blocked with 5% fat‑free milk and probed with anti‑AT1R (1:600).

Zhu Y, et al. The Effect and Mechanism of Chinese Herbal Formula Sini Tang in Heart Failure after Myocardial Infarction in Rats (2018) Evid Based Complement Alternat Med  5629342.  AB-N27AP:  Angiotensin IIR (AT1R)

Objective: To investigate the effectiveness and mechanism of theChinese herbal formula Sini Tang (SNT) in heart failure after myocardial infarction in rats.
Summary: The Chinese herbal formula SNT could improve left ventricular systolic function in heart failure after myocardial infarction.
Dose: Western blot.

Morgan BJ, Schrimpf N, Rothman M, Mitzey A, Brownfield MS, Speth RC, & Dopp JM. Effect of Chronic Intermittent Hypoxia on Angiotensin Ii Receptors in the Central Nervous System. (2018). Clinical and Experimental Hypertension1-7.  AB-N28AP:  Angiotensin AT-2R and AB-N28AP: AT1R.  

Dose:  Antibodies were diluted in the blocking solution with 0.05% sodium azide at the following concentrations:  AT1R, (1:1600) and AT2R, (1:5000).

Johnson PL, Sajdyk TJ, Fitz SD, Hale MW, Lowry CA, Hay-Schmidt A, & Shekhar A. Angiotensin II’s role in sodium lactate-induced panic-like responses in rats with repeated urocortin 1 injections into the basolateral amygdala: Amygdalar angiotensin receptors and panic. (2013). Progress in Neuro-Psychopharmacol Biol Psychiatr, 44 248-256. AB-N27AP: Angiotensin IIR (AT1R)

Summary: The presence of A-II 1 receptor in the basolateral amygdala was confirmed.
Dose: Serial coronal sections (30 μm) were cut to yield three alternate sets of sections. Sections were stored at −20 °C until immunohistochemical processing. All solutions had a pH of 7.4. Sections were incubated overnight at room temperature (1:250 to 1:500 dilution).

Premer C, Lamondin C, Mitzey A, Speth RC, Brownfield MS. (2013) Immunohistochemical Localization of AT1a, AT1b, and AT2 Angiotensin II Receptor Subtypes in the Rat Adrenal, Pituitary, and Brain with a Perspective Commentary. Int J Hypertens 2013:175428. (Targeting Trends 14q4)

Shekhar A, Johnson PL, Sajdyk TJ, Fitz SD, Keim SR, Kelley PE, Gehlert DR, DiMicco JA (2006) Angiotensin-II is a putative neurotransmitter in lactate-induced panic-like responses in rats with disruption of GABAergic inhibition in the dorsomedial hypothalamus. J Neurosci 26(36):9205-9215.

Huang J, Hara Y, Anrather J, Speth RC, Iadecola C, Pickel VM (2003) Angiotensin II subtype 1A (AT1A) receptors in the rat sensory vagal complex: Subcellular localization and association with endogenous angiotensin. Neuroscience 122(1):21-36.

Speth RC, Grove KL, Brownfield MS (2001) Immunohistochemical localization of AT-1A and AT-1B angiotensin II receptor subtypes in the rat adrenal. Endocrine Soc Mtg, Denver CO, Abstract.

Wang G, Anrather J, Huang J, Speth RC, Pickel VM, Iadecola C (2004) NADPH oxidase contributes to angiotensin II signaling in the nucleus tractus solitarius. J Neurosci 24(24):5516-5524. (Targeting Trends 04q4)