NGFr (ME20.4, p75) Mouse Monoclonal References

NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)

29 entries found for : ab-n07

Immunological properties of neural crest cells derived from human induced pluripotent stem cells.

Fujii S, Yoshida S, Inagaki E, Hatou S, Tsubota K, Takahashi M, Shimmura S, Sugita S (2019) Immunological properties of neural crest cells derived from human induced pluripotent stem cells. Stem Cells Dev 28(1):28-43. doi: 10.1089/scd.2018.0058

Usage: IHC; 1:200 dilution

Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)

Selective laminin-directed differentiation of human induced pluripotent stem cells into distinct ocular lineages

Shibata S, Hayashi R, Okubo T, Kudo Y, Katayama T, Ishikawa Y, Toga J, Yagi E, Honma Y, Quantock AJ, Sekiguchi K, Nishida K (2018) Selective laminin-directed differentiation of human induced pluripotent stem cells into distinct ocular lineages. Cell Rep 25:1668-1679. doi: 10.1016/j.celrep.2018.10.032

Usage: Immunostaining, flow cytometry

Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)

Induced pluripotent stem cells with NOTCH1 gene mutation show impaired differentiation into smooth muscle and endothelial cells: Implications for bicuspid aortic valve-related aortopathy

Jiao J, Tian W, Qiu P, Norton EL, Wang MM, Chen YE, Yang B (2018) Induced pluripotent stem cells with NOTCH1 gene mutation show impaired differentiation into smooth muscle and endothelial cells: Implications for bicuspid aortic valve-related aortopathy. J Thorac Cardiovasc Surg 156:515-522. doi: 10.1016/j.jtcvs.2018.02.087

Objective: To develop an in vitro model with human-induced pluripotent stem cells (iPSCs) to evaluate the role of NOTCH1 in smooth muscle and endothelial cell differentiation.

Summary: NOTCH1 is critical in SMC and EC differentiation of iPSCs through neural crest stem cells and cardiovascular progenitor cells, respectively. NOTCH1gene mutations may potentially contribute to the development of thoracic aortic aneurysms by affecting SMC differentiation in some patients with bicuspid aortic valve-related aortopathy.

Usage: Immunofluorescence staining and flow cytometry was performed.

Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)

Extracellular matrix from periodontal ligament cells could induce the differentiation of induced pluripotent stem cells to periodontal ligament stem cell-like cells

Hamano S, Tomokiyo A, Hasegawa D, Yoshida S, Sugii H, Mitarai H, Fujino S, Wada N, Maeda H (2017) Extracellular matrix from periodontal ligament cells could induce the differentiation of induced pluripotent stem cells to periodontal ligament stem cell-like cells. Stem Cells Dev 27:100-111. doi: 10.1089/scd.2017.0077

Usage: Immunofluorescence staining (1:200 dilution)

Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)

Directed differentiation of periocular mesenchyme from human embryonic stem cells

Lovatt M, Yam GH-F, Peh GS, Colman A, Dunn NR, Mehta JS (2018) Directed differentiation of periocular mesenchyme from human embryonic stem cells. Differentiation 99:62-69. doi: 10.1016/j.diff.2017.11.003

Objective: Pluripotent stem cells are attractive sources of cells for regenerative medicine, because large numbers of therapeutically useful cells can be generated. However, a detailed understanding of how to differentiate clinically relevant cell types from stem cells is fundamentally required.

Summary: Identification of cells resembling periocular mesenchyme (POM) cells in the adult cornea, located in a niche between the trabecular meshwork and peripheral endothelium. The generation and expansion of POM is an important step in the generation of a number of cells types that could prove to be clinically useful for a number of diseases of the cornea.

Usage: 1:200 for flow cytometry and immunofluorescence.

Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)

Coordinated generation of multiple ocular-like cell lineages and fabrication of functional corneal epithelial cell sheets from human iPS cells

Hayashi R, Ishikawa Y, Katori R, Sasamoto Y, Taniwaki Y, Takayanagi H, Tsujikawa M, Sekiguchi K, Quantock AJ, Nishida K (2017) Coordinated generation of multiple ocular-like cell lineages and fabrication of functional corneal epithelial cell sheets from human iPS cells. Nat Protoc 12:683-696. doi: 10.1038/nprot.2017.007

Usage: IHC 1:100

Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)

Derivation of corneal keratocyte-like cells from human induced pluripotent stem cells.

Naylor R, McGhee C, Cowan C, Davidson A, Holm T, Sherwin T (2016) Derivation of corneal keratocyte-like cells from human induced pluripotent stem cells. PLoS One 11:e0165464. doi: 10.1371/journal.pone.0165464

Summary: Slides containing cryosections were dried overnight at 4°C and then washed twice in Tris Buffered Saline containing 0.1% Triton X100 (TBST). Slides were then placed in block solution (3% BSA, 5% Goat serum in TBST) for at least one hour. The primary antibody was then applied in the same block solution (1:100) and left overnight at 4°C.

Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)

Differentiation defect in neural crest-derived smooth muscle cells in patients with aortopathy associated with bicuspid aortic valves.

Jiao J, Xiong W, Wang L, Yang J, Qiu P, Hirai H, Shao L, Milewicz D, Chen Y, Yang B (2016) Differentiation defect in neural crest-derived smooth muscle cells in patients with aortopathy associated with bicuspid aortic valves. EBioMedicine 10:282-290. doi: 10.1016/j.ebiom.2016.06.045

Summary: Individuals with bicuspid aortic valves (BAV) are at a higher risk of developing thoracic aortic aneurysms (TAA) than patients with trileaflet aortic valves (TAV). Aneurysms associated with BAV most commonly involve the ascending aorta. Smooth muscle cells (SMCs) in the ascending and descending aorta arise from neural crest (NC) and paraxial mesoderm (PM), respectively. Scientists hypothesized defective differentiation of the neural crest stem cells (NCSCs)-derived SMCs but not paraxial mesoderm cells (PMCs)- derived SMCs contributes to the aortopathy associated with BAV. Induced pluripotent stem cells (iPSCs) from BAV/TAA patients were differentiated into NCSC-derived SMCs and showed decreased expression of a marker of SMC differentiation (MYH11) and impaired contraction. The scientists demonstrated that decreased differentiation and contraction of patient’s NCSC-derived SMCs may contribute to the aortopathy associated with BAV.

Usage: Anti-NGFr (ME20.4, p75, Cat. #AB-N07) was used for the immunofluorescence staining and flow cytometry of NCSCs.

Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)

Bone morphogenetic protein 4 promotes craniofacial neural crest induction from human pluripotent stem cells.

Mimura S, Suga M, Okada K, Kinehara M, Nikawa H, Furue M (2016) Bone morphogenetic protein 4 promotes craniofacial neural crest induction from human pluripotent stem cells. Int J Dev Biol 60:21-28. doi: 10.1387/ijdb.160040mk

Usage: Immunocytochemistry and flow cytometry

Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)

Efficient generation of human embryonic stem cell-derived corneal endothelial cells by directed differentiation

McCabe KL, Kunzevitzky NJ, Chiswell BP, Xia X, Goldberg JL, Lanza R (2015) Efficient generation of human embryonic stem cell-derived corneal endothelial cells by directed differentiation. PLoS One 10(12):e0145266. doi: 10.1371/journal.pone.0145266

Usage: For immunostaining of the expression of Zona Occludens protein 1 (ZO-1), von Willebrand factor (vWF), p75/NGFR and CD31, traditional methods were utilized. Anti-NGFr (p75) 1:100.

Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)

Cell-fate determination by ubiquitin-dependent regulation of translation

Werner A, Iwasaki S, McGourty CA, Medina-Ruiz S, Teerikorpi N, Fedrigo I, Ingolia NT, Rape M (2015) Cell-fate determination by ubiquitin-dependent regulation of translation. Nature 525(7570):523-527. doi: 10.1038/nature14978

Usage: Immunofluorescence 1:100

Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)

The cholinergic basal forebrain in the ferret and its inputs to the auditory cortex.

Bajo V, Leach N, Cordery P, Nodal F, King A (2014) The cholinergic basal forebrain in the ferret and its inputs to the auditory cortex. Eur J Neurosci 40:2922-2940. doi: 10.1111/ejn.12653

Summary: The ferret has become a more common animal model in auditory neuroscience. Unlike rodent models, however, anatomical data describing the organization of the basal forebrain cholinergic system and its projections to the auditory cortex have not been well characterized. Using a variety of methods the authors mapped the architecture of the ferret basal forebrain. IHC was done with several antibodies including anti-ChAT (Cat. #AB-N34AP; 1:1000) and anti-NGFr (Cat. #AB-N07; 1:500). Animals also received 17 μg of ME20.4-SAP (Cat. #IT-15) in a total of 17 injections into the ectosylvian gyrus. The results indicate that acetylcholine is most likely involved in modulation of auditory processing.

Related Products: Choline Acetyltransferase Rabbit Polyclonal, affinity-purified (Cat. #AB-N34AP), NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07), ME20.4-SAP (Cat. #IT-15)

Feeder-free derivation of neural crest progenitor cells from human pluripotent stem cells

Zeltner N, Lafaille FG, Fattahi F, Studer L (2014) Feeder-free derivation of neural crest progenitor cells from human pluripotent stem cells. J Vis Exp 87:51609. doi: 10.3791/51609

Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)

A robust method to derive functional neural crest cells from human pluripotent stem cells

Kreitzer FR, Salomonis N, Sheehan A, Huang M, Park JS, Spindler MJ, Lizarraga P, Weiss WA, So PL, Conklin BR (2013) A robust method to derive functional neural crest cells from human pluripotent stem cells. Am J Stem Cells 2(2):119-131.

Usage: Immunocytochemistry 1:200

Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)

Directed differentiation of human pluripotent cells to neural crest stem cells

Menendez L, Kulik MJ, Page AT, Park SS, Lauderdale JD, Cunningham ML, Dalton S (2013) Directed differentiation of human pluripotent cells to neural crest stem cells. Nat Protoc 8(1):203-212. doi: 10.1038/nprot.2012.156

Summary: This protocol describes in detail how to perform a highly efficient, lineage-specific differentiation of human pluripotent cells to a NCSC fate.

Usage: 0.2 ul per 10^6 cells

Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)

Generation of human melanocytes from induced pluripotent stem cells

Ohta S, Imaizumi Y, Okada Y, Akamatsu W, Kuwahara R, Ohyama M, Amagai M, Matsuzaki Y, Yamanaka S, Okano H, Kawakami Y (2011) Generation of human melanocytes from induced pluripotent stem cells. PLoS One 6(1):e16182. doi: 10.1371/journal.pone.0016182

Summary: For immunocytochemistry, cells were fixed with PBS containing 4% PFA for 20 min at room temperature. Then, cells were subjected to immunofluorescence staining (1∶100).

Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)

Derivation of neural crest cells from human pluripotent stem cells

Lee G, Chambers SM, Tomishima MJ, Studer L (2010) Derivation of neural crest cells from human pluripotent stem cells. Nat Protoc 5(4):688-701. doi: 10.1038/nprot.2010.35

Summary: Protocols are presented for the purification and propagation of hPSC-NC cells using flow cytometry and defined in vitro culture conditions. This protocol has been validated in multiple independent hESC and hiPSC lines. The average time required for generating purified hPSC-NC precursors using this protocol is 2–5 weeks.

Usage: Neural crest cells (1:200).

Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)

In vivo labeling of rabbit cholinergic basal forebrain neurons with fluorochromated antibodies.

Hartig W, Varga C, Kacza J, Grosche J, Seeger J, Luiten PG, Brauer K, Harkany T (2002) In vivo labeling of rabbit cholinergic basal forebrain neurons with fluorochromated antibodies. NeuroReport 13(11):1395-1398. doi: 10.1097/00001756-200208070-00009

Summary: To investigate in vivo labeling of p75 low-affinity neurotrophin receptor the authors conjugated Cy3 to ME20.4 (Cat. #AB-N07) and performed either unilateral or bilateral icv injections in rabbits. The antibody labeled only cholinergic neurons demonstrating its potential as a p75 marker.

Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)

Distribution and co-localization of choline acetyltransferase and p75 neurotrophin receptors in the sheep basal forebrain: implications for the use of a specific cholinergic immunotoxin.

Ferreira G, Meurisse M, Tillet Y, Lévy F (2001) Distribution and co-localization of choline acetyltransferase and p75 neurotrophin receptors in the sheep basal forebrain: implications for the use of a specific cholinergic immunotoxin. Neuroscience 104(2):419-439. doi: 10.1016/s0306-4522(01)00075-6

Summary: ME20.4 is a monoclonal antibody (Cat. #AB-N07) that has been shown to bind the p75 receptor in rabbit, sheep, dog, cat, raccoon, pig, and several primate species. Ferreira et al. investigate ME20.4-SAP (bilateral, 150 µl per ventricle, 50-150 µg total; Cat. #IT-15) use in sheep to assess distribution and localization of p75. The authors demonstrate 80-95% loss of basal forebrain cholinergic neurons and acetylcholinesterase-positive fibers in the hippocampus, olfactory bulb, and entorhinal cortex.

Related Products: ME20.4-SAP (Cat. #IT-15), NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)

Antibody for human p75 LNTR identifies cholinergic basal forebrain of non-primate species.

Tremere LA, Pinaud R, Grosche J, Hartig W, Rasmusson DD (2000) Antibody for human p75 LNTR identifies cholinergic basal forebrain of non-primate species. NeuroReport 11(10):2177-2183. doi: 10.1097/00001756-200007140-00023

Summary: 192-SAP (Cat. #IT-01) is a highly successful reagent for eliminating cholinergic neurons in rats. Because the targeting antibody only recognizes rat p75, it is unable to be used in other species. Tremere et al. have stained basal forebrain sections with ME20.4, a monoclonal antibody to human p75 (Cat. #AB-N07) and found excellent cross-reactivity in dog, raccoon, cat, pig and rabbit. The authors state that an ME20.4-saporin conjugate could be used to produce cholinergic basal forebrain lesions in several species.

Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07), 192-IgG-SAP (Cat. #IT-01)

Immunohistochemical detection of p140trkA and p75LNGFR neurotrophin receptors in neuroblastoma.

Dominici C, Nicotra MR, Alema S, Bosman C, Castello MA, Donfrancesco A, Gallo P, McDowell H, Natali PG (1997) Immunohistochemical detection of p140trkA and p75LNGFR neurotrophin receptors in neuroblastoma. J Neurooncol 31:57-64. doi: 10.1023/a:1005781313596

Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)

Immuno-electron microscopy characterization of human bone marrow stromal cells with anti-NGFR antibodies.

Caneva L, Soligo D, Cattoretti G, De Harven E, Deliliers GL (1995) Immuno-electron microscopy characterization of human bone marrow stromal cells with anti-NGFR antibodies. Blood Cells Mol Dis 21:73-85. doi: 10.1006/bcmd.1995.0011

Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)

Low-affinity nerve growth factor receptor immunoreactivity in the human urinary bladder.

Wakabayashi Y, Tomoyoshi T, Tooyama I, Kitahama K, Kim SU, Maeda T (1995) Low-affinity nerve growth factor receptor immunoreactivity in the human urinary bladder. Neurosci Lett 186:9-12. doi: 10.1016/0304-3940(95)11268-2

Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)

Nerve growth factor receptor expression in fetal, mature, and diseased human kidneys.

Alpers CE, Hudkins KL, Ferguson M, Johnson RJ, Schatteman GC, Bothwell M (1993) Nerve growth factor receptor expression in fetal, mature, and diseased human kidneys. Lab Invest 69:703-713.

Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)

Cholinergic innervation of the human striatum, globus pallidus, subthalamic nucleus, substantia nigra, and red nucleus.

Mesulam MM, Mash D, Hersh L, Bothwell M, Geula C (1992) Cholinergic innervation of the human striatum, globus pallidus, subthalamic nucleus, substantia nigra, and red nucleus. J Comp Neurol 323:252-268. doi: 10.1002/cne.903230209

Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)

Localization of NGF receptors in normal and Alzheimer’s basal forebrain with monoclonal antibodies against the truncated form of the receptor.

Loy R, Heyer D, Clagett-Dame M, DiStefano PS (1990) Localization of NGF receptors in normal and Alzheimer's basal forebrain with monoclonal antibodies against the truncated form of the receptor. J Neurosci Res 27:651-664. doi: 10.1002/jnr.490270426

Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)

A monoclonal antibody against nerve growth factor receptor.

Thompson SJ, Schatteman GC, Gown AM, Bothwell M (1989) A monoclonal antibody against nerve growth factor receptor. Am J Clin Pathol 92:415-423. doi: 10.1093/ajcp/92.4.415

Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)

Purification and amino terminal sequencing of human melanoma nerve growth factor receptor.

Marano N, Dietzschold B, Earley JJ Jr, Schatteman G, Thompson S, Grob P, Ross AH, Bothwell M, Atkinson BF, Koprowski H (1987) Purification and amino terminal sequencing of human melanoma nerve growth factor receptor. J Neurochem 48:225-232. doi: 10.1111/j.1471-4159.1987.tb13151.x

Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)

Characterization of nerve growth factor receptor in neural crest tumors using monoclonal antibodies.

Ross AH, Grob P, Bothwell M, Elder DE, Ernst CS, Marano N, Ghrist BFD, Slemp CC, Herlyn M, Atkinson B, Koprowski H (1984) Characterization of nerve growth factor receptor in neural crest tumors using monoclonal antibodies. Proc Natl Acad Sci U S A 81:6681-6685. doi: 10.1073/pnas.81.21.6681

Related Products: NGFr (ME20.4, p75) Mouse Monoclonal (Cat. #AB-N07)