trkA Rabbit Polyclonal References

trkA Rabbit Polyclonal (Cat. #AB-N03)

12 entries found for : ab-n03

KDM6A promotes imatinib resistance through YY1-mediated transcriptional upregulation of TRKA independently of its demethylase activity in chronic myelogenous leukemia

Zhang C, Shen L, Zhu Y, Xu R, Deng Z, Liu X, Ding Y, Wang C, Shi Y, Bei L, Wei D, Thorne RF, Zhang XD, Yu L, Chen S (2021) KDM6A promotes imatinib resistance through YY1-mediated transcriptional upregulation of TRKA independently of its demethylase activity in chronic myelogenous leukemia. Theranostics 11(6):2691-2705. doi: 10.7150/thno.50571

Objective: To identify histone lysine demethylases (KDMs) with altered expression in chronic myelogenous leukemia (CML) and define their contribution to imatinib resistance.

Summary: KDM6A promotes imatinib-resistance in CML cells. The identification of the KDM6A/YY1/TRKA axis as a novel imatinib-resistance mechanism represents an unexplored avenue to overcome tyrosine kinase inhibitor resistance in CML.

Usage: Western blot

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The impact of bone cancer on the peripheral encoding of mechanical pressure stimuli.

Kucharczyk MW, Chisholm KI, Denk F, Dickenson AH, Bannister K, McMahon SB (2020) The impact of bone cancer on the peripheral encoding of mechanical pressure stimuli. Pain 161(8):1894-1905. doi: 10.1097/j.pain.0000000000001880

Usage: immunohistochemistry (1:400)

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The impact of bone cancer on the peripheral encoding of mechanical pressure stimuli

Kucharczyk MW, Chisholm KI, Denk F, Dickenson AH, Bannister K, McMahon SB (2019) The impact of bone cancer on the peripheral encoding of mechanical pressure stimuli. bioRxiv 498980. doi: 10.1101/498980

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The reduced level of growth factors in an animal model of depression is accompanied by regulated necrosis in the frontal cortex but not in the hippocampus

Kucharczyk M, Kurek A, Pomierny B, Detka J, Papp M, Tota K, Budziszewska B (2018) The reduced level of growth factors in an animal model of depression is accompanied by regulated necrosis in the frontal cortex but not in the hippocampus. Psychoneuroendocrinology 94:121-133. doi: 10.1016/j.psyneuen.2018.05.008

Objective: To investigate if the different types of stress alter neuronal plasticity markers distinctively in the frontal cortex (FCx) and in the hippocampus (Hp).

Summary: The authors concluded that chronic stress during pregnancy can result in serious alterations in the functioning of the FCx of the progeny, facilitating the development of depressive behavior later in life. They also suggest that the altered energy metabolism may redirect pro-NGF/p75NTR/ATF2 signaling in the cortical neurons towards cellular death resembling regulated necrosis, rather than apoptosis.

Usage: Western blot (1:1000)

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TrkA in vivo function is negatively regulated by ubiquitination.

Kiris E, Wang T, Yanpallewar S, Dorsey S, Becker J, Bavari S, Palko M, Coppola V, Tessarollo L (2014) TrkA in vivo function is negatively regulated by ubiquitination. J Neurosci 34:4090-4098. doi: 10.1523/JNEUROSCI.4294-13.2014

Summary: The high affinity nerve growth factor receptor, trkA, plays an intrinsic role in the regulation of various aspects of the mammalian nervous system. The post-translational attachment of ubiquitin to trkA plays a role in the final disposition and function of many proteins; in this work the authors investigate the result of trkA ubiquitination. By removing a 3 amino acid sequence from the receptor the ubiquitination of TrkA was reduced which resulted in an increase in TrkA protein levels and activity. In mice containing this mutation, the rise in TrkA activity was accompanied by enhanced thermal sensitivity and inflammatory pain. Anti-trkA (Cat. #AB-N03) was used at a concentration of 1:500 in immunohistochemistry.

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c-Maf is required for the development of dorsal horn laminae III/IV neurons and mechanoreceptive DRG axon projections.

Hu J, Huang T, Li T, Guo Z, Cheng L (2012) c-Maf is required for the development of dorsal horn laminae III/IV neurons and mechanoreceptive DRG axon projections. J Neurosci 32(16):5362-5373. doi: 10.1523/JNEUROSCI.6239-11.2012

Summary: The molecular mechanisms responsible for development of laminae III/IV neurons are not yet well understood. In this work the authors investigated the role of c-Maf, a basic leucine-zipper transcription factor from the AP-1 superfamily. Anti-TrkA (Cat. #AB-N03: 1:100) was used for immunohistochemistry.

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TrkA gene ablation in basal forebrain results in dysfunction of the cholinergic circuitry.

Sanchez-Ortiz E, Yui D, Song D, Li Y, Rubenstein JL, Reichardt LF, Parada LF (2012) TrkA gene ablation in basal forebrain results in dysfunction of the cholinergic circuitry. J Neurosci 32(12):4065-4079. doi: 10.1523/JNEUROSCI.6314-11.2012

Summary: The authors created a conditional trkA knockout mouse line. Anti-trkA (Cat. #AB-N03) was used for immunohistochemistry (1:1000) and western blots (1:4000). The data demonstrate the importance of trkA in the establishment of basal forebrain cholinergic circuitry, and choline acetyltransferase expression.

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Proteomic analysis uncovers novel actions of the neurosecretory protein VGF in nociceptive processing.

Riedl MS, Braun PD, Kitto KF, Roiko SA, Anderson LB, Honda CN, Fairbanks CA, Vulchanova L (2009) Proteomic analysis uncovers novel actions of the neurosecretory protein VGF in nociceptive processing. J Neurosci 29:13377-13388. doi: 10.1523/JNEUROSCI.1127-09.2009

Summary: Peripheral tissue injury can alter protein expression in sensory neurons, which may contribute to abnormal nociceptive processing. The authors used cultured dorsal root ganglion neurons as a model for axotomized neurons to examine early changes in protein expression after nerve injury. Several different parameters were measured, including immunohistochemistry using anti-TrkA (Cat. #AB-N03). The data show an increased level of a putative neuropeptide precursor, VGF, as a result of nerve injury.

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Hippocampal Trk-A receptors are compartmentalized within axonal transport vesicle sites and present in select astrocytes.

Barker-Gibb ML, Einheber S, Milner TA (1999) Hippocampal Trk-A receptors are compartmentalized within axonal transport vesicle sites and present in select astrocytes. Neuroscience 1999 Abstracts 803.6. Society for Neuroscience, Miami, FL.

Summary: Trk-A is a high affinity transmembrane tyrosine kinase receptor which is activated by its ligand, nerve growth factor (NGF), to mediate cell survival and the retrograde transport of NGF by septohippocampal cholinergic neurons. To determine its subcellularlocalization, antibodies to the extracellular domain of the Trk-A receptor (Chemicon; Upstate) were localized immunocytochemically in rat dentate gyrus by light & electron microscopy (LM; EM). These antibodies recognized two bands migrating at -125 and 140 kD on immunoblots of septal and hippocampal lysates. Consistent with previous LM reports, Trk-A-immuno- reactivity (-I) with both antibodies was found in fine, varicose fibers, primarily in the hilus. However, the Chemicon antibody also labeled fibers in the granule and molecular layers (ML) as well as numerous astrocytic profiles in the external and medial zones of the ML and the superficial blade of the hilus. EM analysis of the dentate gyrus using the Chemicon antibody revealed that Trk-A-I was in axons, axon terminals and astrocytes. Trk-A-I in axons was not homogeneous, but was concentrated in patches near regions where vesicles accumulated. Trk-A-I in terminals was affiliated with clusters of small synaptic vesicles frequently located away from synaptic specializations. In both axons and axon terminals, the clusters of Trk-A-labeled vesicles often were adjacent to somatic and dendritic profiles. Trk-A-labeled astrocytes in the hilus regularly closely apposed terminals presynaptic to dendritic spines. The findings support evidence that hippocampal Trk-A receptors are compartmentalized within transport vesicle sites. Also, they suggest that astrocytes play a role in the uptake and retrograde transport of NGF by septohippocampal cholinergic neurons to support their survival. (Support: MH42834; DA 08259)

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A role for nerve growth factor in sympathetic sprouting in rat dorsal root ganglia.

Jones MG, Munson JB, Thompson SW (1999) A role for nerve growth factor in sympathetic sprouting in rat dorsal root ganglia. Pain 79(1):21-29. doi: 10.1016/S0304-3959(98)00142-0

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TrkA-immunoreactive profiles in the central nervous system: colocalization with neurons containing p75 nerve growth factor receptor, choline acetyltransferase, and serotonin.

Sobreviela T, Clary DO, Reichardt LF, Brandabur MM, Kordower JH, Mufson EJ (1994) TrkA-immunoreactive profiles in the central nervous system: colocalization with neurons containing p75 nerve growth factor receptor, choline acetyltransferase, and serotonin. J Comp Neurol 350:587-611. doi: 10.1002/cne.903500407

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TrkA cross-linking mimics neuronal responses to nerve growth factor.

Clary DO, Weskamp G, Austin LR, Reichardt LF (1994) TrkA cross-linking mimics neuronal responses to nerve growth factor. Mol Biol Cell 5:549-563. doi: 10.1091/mbc.5.5.549

Related Products: trkA Rabbit Polyclonal (Cat. #AB-N03)