NGFr (mu p75) Rabbit Polyclonal References

NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01), NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

30 entries found for : ab-n01

Toll-like receptor 4-mediated enteric glia loss is critical for the development of necrotizing enterocolitis

Kovler ML, Gonzalez Salazar AJ, Fulton WB, Lu P, Yamaguchi Y, Zhou Q, Sampah M, Ishiyama A, Prindle T Jr, Wang S, Jia H, Wipf P, Sodhi CP, Hackam DJ (2021) Toll-like receptor 4-mediated enteric glia loss is critical for the development of necrotizing enterocolitis. Sci Transl Med 13(612):eabg3459. doi: 10.1126/scitranslmed.abg3459

Objective: Hypothesized that enteric glia loss in the premature intestine could lead to dysmotility, exaggerated Toll-like receptor 4 (TLR4) signaling and necrotizing enterocolitis (NEC) development.

Summary: TLR4-induced enteric glia loss leads to experimental NEC through Brain Derived Neurotrophic Factor (BDNF) loss, which can be treated with compound 'J11.'

Usage: Immunofluorescence

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Dorso-ventral heterogeneity in tracheal basal stem cells

Tadokoro T, Tanaka K, Osakabe S, Kato M, Kobayashi H, Hogan BLM, Taniguchi H (2021) Dorso-ventral heterogeneity in tracheal basal stem cells. Biol Open 10(9):bio058676. doi: 10.1242/bio.058676

Objective: To determine whether tracheal basal cells (BCs) from the dorso-ventral airways have intrinsic molecular and behavioural differences relevant to their in vivo function.

Summary: This study revealed the differences in the characteristics of BCs based on spatial distribution, and also indicated the role of epithelial-mesenchymal interactions in tissue homoeostasis in the trachea. The findings of this study suggest that careful observation is necessary in future research, as the influence of environment on the phenomenon should be considered.

Usage: Immunohistochemistry (1:100)

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Airway basal stem cells reutilize the embryonic proliferation regulator, Tgfβ-Id2 axis, for tissue regeneration

Kiyokawa H, Yamaoka A, Matsuoka C, Tokuhara T, Abe T, Morimoto M (2021) Airway basal stem cells reutilize the embryonic proliferation regulator, Tgfβ-Id2 axis, for tissue regeneration. Dev Cell 56(13):1917-1929.e9. doi: 10.1016/j.devcel.2021.05.016

Objective: To investigate the molecular mechanisms regulating how basal cells contribute to adult tissue regeneration by shifting from slow cycling to proliferating and subsequently back to slow cycling.

Summary: TGF-β-Id2 axis commonly regulates the proliferation transitions in basal cells during development and regeneration, and its fine-tuning is critical for normal regeneration while avoiding basal cell hyperplasia.

Usage: Immunostaining

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Premigratory neural crest stem cells generate enteric neurons populating the mouse colon and regulating peristalsis in tissue-engineered intestine

Yuan H, Hu H, Chen R, Mu W, Wang L, Li Y, Chen Y, Ding X, Xi Y, Mao S, Jiang M, Chen J, He Y, Wang L, Dong Y, Tou J, Chen W (2021) Premigratory neural crest stem cells generate enteric neurons populating the mouse colon and regulating peristalsis in tissue-engineered intestine. Stem Cells Transl Med 10(6):922-938. doi: 10.1002/sctm.20-0469

Objective: To determine whether premigratory NCSCs (pNCSCs) could form enteric neurons and mediate the motility.

Summary: The results show that the pNCSCs that were previously assumed to not be induced by intestinal environment or cues can innervate the intestine and establish neuron-dependent motility.

Usage: Immunochemistry (1:200)

Related Products: NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01)

Peripheral nerve regeneration with 3D printed bionic scaffolds loading neural crest stem cell derived Schwann cell progenitors

Li Y, Lv S, Yuan H, Ye G, Mu W, Fu Y, Zhang X, Feng Z, He Y, Chen W (2021) Peripheral nerve regeneration with 3D printed bionic scaffolds loading neural crest stem cell derived Schwann cell progenitors. Advanced Functional Materials 31(16):2010215. doi: 10.1002/adfm.202010215

Objective: Regeneration efficiency and behavior of peripheral nerves are compared under three treatment strategies: 1) transplantation of Schwann cell progenitors induced from purified neural crest stem cells; 2) implantation of a multiscale scaffold based on high-resolution 3D printing; and 3) implantation of this bionic scaffold loading Schwann cell progenitors.

Summary: Implantation of multiscale scaffolds preloaded with neural crest stem cell derived Schwann cell progenitors is the best strategy to promote peripheral nerve regeneration with improved anatomy and function among the three different strategies.

Usage: Immunostaining (1:200)

Related Products: NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01)

Sox10 functions as an inducer of the direct conversion of keratinocytes into neural crest cells

Motohashi T, Kawamura N, Watanabe N, Kitagawa D, Goshima N, Kunisada T (2020) Sox10 functions as an inducer of the direct conversion of keratinocytes into neural crest cells. Stem Cells Dev 29(23):1510-1519. doi: 10.1089/scd.2020.0106

Objective: To investigate whether Sox10 functions in the direct conversion of other somatic cells into neural crest cells (NCCs).

Summary: Results demonstrate that Sox10 functions as an inducer of direct conversion into NCCs in cooperation with the transcription factors involved in NCC generation.

Usage: Flow cytometry and cell sorting

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Gli1+ mesenchymal stromal cells form a pathological niche to promote airway progenitor metaplasia in the fibrotic lung

Cassandras M, Wang C, Kathiriya J, Tsukui T, Matatia P, Matthay M, Wolters P, Molofsky A, Sheppard D, Chapman H, Peng T (2020) Gli1+ mesenchymal stromal cells form a pathological niche to promote airway progenitor metaplasia in the fibrotic lung. Nat Cell Biol 22(11):1295-1306. doi: 10.1038/s41556-020-00591-9

Objective: To show that Gli1+ mesenchymal stromal cells (MSCs) promote metaplastic differentiation of airway progenitors into KRT5+ basal cells.

Summary: Gli1+ MSCs integrate hedgehog signaling as a rheostat to control BMP activation in the progenitor niche to determine regenerative outcome in fibrosis.

Usage: Histology and Immunofluorescence (1:200)

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Cell lineage tracing identifies hormone-regulated and wnt-responsive vaginal epithelial stem cells

Ali A, Syed SM, Jamaluddin MFB, Colino-Sanguino Y, Gallego-Ortega D, Tanwar PS (2020) Cell lineage tracing identifies hormone-regulated and wnt-responsive vaginal epithelial stem cells. Cell Rep 30(5):1463-1477.e7. doi: 10.1016/j.celrep.2020.01.003

Objective: To learn more about the role of Wnt signaling in vaginal epithelium (VE) homeostasis.

Summary: Data define heterogeneity in VE and identify VE stem cells. It was shown that canonical Wnt/b-catenin signaling is required for the proliferation and differentiation of VE stem cells.

Usage: histology and immunostaining (1:250)

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Development of intestinal scaffolds that mimic native mammalian intestinal tissue

Ladd MR, Costello CM, Gosztyla C, Werts AD, Johnson B, Fulton WB, Martin LY, Redfield EJ, Crawford B, Panaparambil R, Sodhi CP, March JC, Hackam DJ (2019) Development of intestinal scaffolds that mimic native mammalian intestinal tissue. Tissue Eng Part A 25(17-18):1225-1241. doi: 10.1089/ten.TEA.2018.0239

Objective: To develop a scaffold for the generation of an artificial intestine that specifically mimics the architecture and biomechanical properties of the native small intestine, and to evaluate the scaffold in vitro and in vivo.

Summary: Novel scaffolds were developed using PGS with promise for use in artificial intestine for individuals with short bowel syndrome.

Usage: Histology, immunohistochemistry, microscopy (1:500)

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Nicotinamide mononucleotide adenylyltransferase 2 maintains neuronal structural integrity through the maintenance of golgi structure.

Pottorf T, Mann A, Fross S, Mansel C, Vohra BPS (2018) Nicotinamide mononucleotide adenylyltransferase 2 maintains neuronal structural integrity through the maintenance of golgi structure. Neurochem Int 121:86-97. doi: 10.1016/j.neuint.2018.09.010

Objective: To Investigate the link between NMNAT2 loss, Golgi fragmentation and axon degeneration

Summary: Results degeneration signals converge on a common cytNmnat1 mediated axon protective program and are distinct from the SARM1 mediated caspase independent axon degeneration

Usage: Immunohistochemistry (1:1000)

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Enteric neurodegeneration is mediated through independent neuritic and somal mechanisms in rotenone and MPP+ toxicity.

Virga DM, Capps J, Vohra BPS (2018) Enteric neurodegeneration is mediated through independent neuritic and somal mechanisms in rotenone and MPP+ toxicity. Neurochem Res 43(12):2288-2303. doi: 10.1007/s11064-018-2649-x

Objective: To investigate the mechanism by which enteric nervous system degeneration occurs in Parkinson's Disease.

Summary: Therapies targeting both axonal and somal protection can be important in finding interventions for enteric symptoms in PD.

Usage: P75NTR antibody utilized (1:1000) to immunoselect enteric neural crest from the rest of the cells in the gut including stomach, small and large bowl and colon) from E12.5 Sprague CD1 mice.

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Non-canonical Ret signaling augments p75-mediated cell death in developing sympathetic neurons

Donnelly CR, Gabreski NA, Suh EB, Chowdhury M, Pierchala BA (2018) Non-canonical Ret signaling augments p75-mediated cell death in developing sympathetic neurons. J Cell Biol 217:327. doi: 10.1083/jcb.201703120

Usage: Proximity ligation assays (1:500); Western blotting (1:1,000)

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Direct conversion of mouse embryonic fibroblasts into neural crest cells

Motohashi T, Kunisada T (2019) Direct conversion of mouse embryonic fibroblasts into neural crest cells. (eds. Turksen K). In: Skin Stem Cells. Methods in Molecular Biology. 1879:307-321. Humana Press, New York, NY. doi: 10.1007/7651_2018_145

Objective: To describe methods for the direct conversion of mouse embryonic fibroblasts into neural crest cells (NCCs).

Summary: Sox10-IRES-Venus mouse fibroblasts were used for the conversion and isolation of converted NCCs as SOX10-positive cells.

Usage: Immunostaining for Flow Cytometry

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Myoepithelial cells of submucosal glands can function as reserve stem cells to regenerate airways after injury

Tata A, Kobayashi Y, Chow RD, Tran J, Desai A, Massri AJ, McCord TJ, Gunn MD, Tata PR (2018) Myoepithelial cells of submucosal glands can function as reserve stem cells to regenerate airways after injury. Cell Stem Cell 22:668-683. doi: 10.1016/j.stem.2018.03.018

Objective: To show that myoepithelial cells (MECs) of the submucosal glands (SMGs) proliferate and migrate to repopulate the airway surface epithelium (SE) in multiple injury models.

Summary: SMG-like cells appear on the SE of extra-and intra-lobular airways of large animal lungs following severe injury. The transcription factor SOX9 is necessary for MEC plasticity in airway regeneration.

Usage: Immunostaining

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The specification and maturation of nociceptive neurons from human embryonic stem cells

Boisvert EM, Engle SJ, Hallowell SE, Liu P, Wang ZW, Li XJ (2015) The specification and maturation of nociceptive neurons from human embryonic stem cells. Sci Rep 5:16821. doi: 10.1038/srep16821

Usage: Immunocytochemistry 1:200

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proBDNF and p75NTR control excitability and persistent firing of cortical pyramidal neurons.

Gibon J, Buckley S, Unsain N, Kaartinen V, Séguéla P, Barker P (2015) proBDNF and p75NTR control excitability and persistent firing of cortical pyramidal neurons. J Neurosci 35:9741-9753. doi: 10.1523/JNEUROSCI.4655-14.2015

Summary: Principal neurons in the entorhinal cortex (EC) display persistent firing (PF) during working-memory tasks. Much of the communication between the hippocampus and the neocortex passes through the EC, and the EC also receives some cholinergic input from the medial septum and diagonal band of Broca. In this work the authors investigated the role of pro-brain-derived neurotrophic factor (proBDNF) and the p75 receptor in excitability and PF in the EC. The authors propose the proBDNF/p75 system as a regulator for pyramidal neuron excitability and PF in the EC, preventing runaway activity. Some of the western blot and current-clamp data was generated using Anti-p75 (Cat. #AB-N01; no concentration information provided).

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BMP9 ameliorates amyloidosis and the cholinergic defect in a mouse model of Alzheimer’s disease.

Burke RM, Norman TA, Haydar TF, Slack BE, Leeman SE, Blusztajn JK, Mellott TJ (2013) BMP9 ameliorates amyloidosis and the cholinergic defect in a mouse model of Alzheimer’s disease. Proc Natl Acad Sci U S A 110(48):19567-19572. doi: 10.1073/pnas.1319297110

Summary: During development bone morphogenetic protein 9 (BMP9) induces the cholinergic phenotype in the basal forebrain. The authors investigated the use of BMP9 as a treatment of basal forebrain cholinergic degeneration, such as is seen in Alzheimer's disease (AD). Transgenic mice displaying AD phenotypes and expressing GFP in cholinergic neurons received icv infusions of BMP9, and several cholinergic markers were assessed. Anti-p75NTR (Cat. #AB-N01) was used in immunoblotting at a 1:3000 dilution to measure p75 levels. The results demonstrate the protective and therapeutic activity of BMP9 on AD symptoms.

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Neurotrophin/receptor expression in urinary bladder of mice with overexpression of NGF in urothelium.

Girard BM, Malley SE, Vizzard MA (2011) Neurotrophin/receptor expression in urinary bladder of mice with overexpression of NGF in urothelium. Am J Physiol Renal Physiol 300(2):F345-55. doi: 10.1152/ajprenal.00515.2010

Summary: Chronic overexpression of nerve growth factor (NGF) in the urinary bladder results in neuronal sprouting, increased voiding frequency, and referred somatic hypersensitivity. The authors investigated several growth factor and receptor responses to chronic overexpression of NGF. A p75 antibody (Cat. #AB-N01) was used to obtain the immunohistochemistry data. The data suggest that changes due to NGF overexpression may be a compensatory attempt to reduce voiding frequency.

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Basal cells as stem cells of the mouse trachea and human airway epithelium

Rock JR, Onaitis MW, Rawlins EL, Lu Y, Clark CP, Xue Y, Randell SH, Hogan BL (2009) Basal cells as stem cells of the mouse trachea and human airway epithelium. Proc Natl Acad Sci U S A 106(31):12771-12775. doi: 10.1073/pnas.0906850106

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The p75 receptor is associated with inflammatory thermal hypersensitivity.

Watanabe T, Ito T, Inoue G, Ohtori S, Kitajo K, Doya H, Takahashi K, Yamashita T (2008) The p75 receptor is associated with inflammatory thermal hypersensitivity. J Neurosci Res 86:3566-3574. doi: 10.1002/jnr.21808

Summary: Inflammation, which can lead to pain hypersensitivity, not only elevates NGF levels, but also can increase the sensitivity of high-threshold receptors. NGF is thought to be a major contributor to heightened pain states due to inflammation, and in this work the authors investigated the role that the p75 neurotrophin receptor might play in NGF-induced hyperalgesia. 30 minutes prior to injection with Freund's adjuvant, mice received 10 μl of polyclonal anti-p75 (Cat. #AB-N01) into the plantar surface of the paw. The antibody administration blocked hyperalgesia, indicating that p75 plays an important role in inflammation-induced hyperalgesia.

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Isolation and directed differentiation of neural crest stem cells derived from human embryonic stem cells

Lee G, Kim H, Elkabetz Y, Al Shamy G, Panagiotakos G, Barberi T, Tabar V, Studer L (2007) Isolation and directed differentiation of neural crest stem cells derived from human embryonic stem cells. Nat Biotechnol 25(12):1468-1475. doi: 10.1038/nbt1365

Usage: Flow Cytometry cell sorting was performed using Anti-NGFr (p75).

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Altered balance of glutamatergic/GABAergic synaptic input and associated changes in dendrite morphology after BDNF expression in BDNF-deficient hippocampal neurons.

Singh B, Henneberger C, Betances D, Arevalo MA, Rodríguez-Tébar A, Meier JC, Grantyn R (2006) Altered balance of glutamatergic/GABAergic synaptic input and associated changes in dendrite morphology after BDNF expression in BDNF-deficient hippocampal neurons. J Neurosci 26(27):7189-7200. doi: 10.1523/JNEUROSCI.5474-05.2006

Related Products: NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01), NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Bone morphogenetic protein 9 induces the transcriptome of basal forebrain cholinergic neurons.

Lopez-Coviella I, Follettie MT, Mellott TJ, Kovacheva VP, Slack BE, Diesl V, Berse B, Thies RS, Blusztajn JK (2005) Bone morphogenetic protein 9 induces the transcriptome of basal forebrain cholinergic neurons. Proc Natl Acad Sci U S A 102(19):6984-6989. doi: 10.1073/pnas.0502097102

Related Products: NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01), NGFr (mu p75) Rabbit Polyclonal, affinity-purified (Cat. #AB-N01AP)

Astrocytic production of nerve growth factor in motor neuron apoptosis: implications for amyotrophic lateral sclerosis

Pehar M, Cassina P, Vargas MR, Castellanos R, Viera L, Beckman JS, Estévez AG, Barbeito L (2004) Astrocytic production of nerve growth factor in motor neuron apoptosis: implications for amyotrophic lateral sclerosis. J Neurochem 89(2):464-473. doi: 10.1111/j.1471-4159.2004.02357.x

Summary: The role of p75-mediated activity evaluated with p75NTR blocking antibody. Motor neuron loss induced by reactive astrocytes was prevented by blocking the p75NTR.

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Mesenchymal cell precursors of peritubular smooth muscle cells of the mouse testis can be identified by the presence of the p75 neurotrophin receptor.

Campagnolo L, Russo MA, Puglianiello A, Favale A, Siracusa G (2001) Mesenchymal cell precursors of peritubular smooth muscle cells of the mouse testis can be identified by the presence of the p75 neurotrophin receptor. Biol Reprod 64(2):464-472. doi: 10.1095/biolreprod64.2.464

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Early migratory rat neural crest cells express functional gap junctions: Evidence that neural crest cell survival requires gap junction function.

Bannerman P, Nichols W, Puhalla S, Oliver T, Berman M, Pleasure D (2000) Early migratory rat neural crest cells express functional gap junctions: Evidence that neural crest cell survival requires gap junction function. J Neurosci Res 61:605-615. doi: 10.1002/1097-4547(20000915)61:6<605::AID-JNR4>3.0.CO;2-U

Summary: Gap junctions are vital for intercellular communication, especially during development. Neural crest cells develop into several types of neural cells, often migrating as a mass of cells to their final destinations. Bannerman et al. use the anti-p75 antibody (Cat. #AB-N01) to confirm the presence of p75 in neural crest cells. The authors examine how crucial survival signals are communicated during migration and demonstrate that interfering with gap junction formation causes death of neural crest cells.

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Identification of a human olfactory ensheathing cell that can effect transplant mediated remyelination of demyelinated CNS axons.

Barnett SC, Alexander CL Iwashita Y, Gilson JM, Crowther J, Clark L, Dunn KT, Papanastassiou V, Kennedy PG, Franklin RJ (2000) Identification of a human olfactory ensheathing cell that can effect transplant mediated remyelination of demyelinated CNS axons. Brain 123:1581-1588. doi: 10.1093/brain/123.8.1581

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Adhesion molecule expression and phenotype of glial cells in the olfactory tract.

Barnett SC, Franceschini IA (1999) Adhesion molecule expression and phenotype of glial cells in the olfactory tract. (eds. Matsas R, Tsacopoulos M). The Functional Roles of Glial Cells in Health and Disease. Advances in Experimental Medicine and Biology. 468:297-307. Springer, Boston, MA. doi: 10.1007/978-1-4615-4685-6_24

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Mesenchymal and neuronal cell expression of the p75 neurotrophin receptor gene occur by different mechanisms.

Huber LJ, Chao MV (1995) Mesenchymal and neuronal cell expression of the p75 neurotrophin receptor gene occur by different mechanisms. Devel Biol 167:227-238. doi: 10.1006/dbio.1995.1019

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High-affinity NGF binding requires coexpression of the trk proto-oncogene and the low-affinity NGF receptor.

Hempstead BL, Martin-Zanca D, Kaplan DR, Parada LF, Chao MV (1991) High-affinity NGF binding requires coexpression of the trk proto-oncogene and the low-affinity NGF receptor. Nature 350(6320):678-683. doi: 10.1038/350678a0

Related Products: NGFr (mu p75) Rabbit Polyclonal (Cat. #AB-N01)