NGFr (mu p75) Rabbit Polyclonal References

Cat #AB-N01, Cat #AB-N01AP


Qin J, Wang M, Zhao T, Xiao X, Li X, Yang J, Yi L, Goffinet AM, Qu Y, & Zhou L. Early Forebrain Neurons and Scaffold Fibers in Human Embryos. (2020) Cerebral Cortex 30(3):913–928. doi: 10.1093/cercor/bhz136

AB-N01AP: Immunofluorescence (1:600)


Katri A, Dąbrowska A, Löfvall H, Ding M, Karsdal MA, Andreassen KV, Thudium CS, & Henriksen K. Combining Naproxen and a Dual Amylin and Calcitonin Receptor Agonist Improves Pain and Structural Outcomes in the Collagen-Induced Arthritis Rat Model. (2019) Arthritis Res Ther. 21(1):68. doi: 10.1186/s13075-019-1819-9

AB-N01AP: histology (1:4000)

Ladd MR, Costello CM, Gosztyla C, Werts AD, Johnson B, Fulton WB, Martin LY, Redfield EJ, Crawford B, Panaparambil R, Sodhi CP, March JC, & Hackam DJ. Development of Intestinal Scaffolds That Mimic Native Mammalian Intestinal Tissue (2019) Tissue Eng Part A 25(17-18):1225-1241. doi: 10.1089/ten.TEA.2018.0239

AB-N01: immunohistochemistry (1:500)

Matamoros AJ, Tom VJ, Wu D, Rao Y, Sharp DJ, & Baas PW. Knockdown of Fidgetin Improves Regeneration of Injured Axons by a Microtubule-Based Mechanism. (2019) J Neurosci 39(11):2011-2024. doi: 10.1523/JNEUROSCI.1888-18.2018

AB-N01AP: immunohistochemistry

Motohashi T, Kunisada T. Direct Conversion of Mouse Embryonic Fibroblasts into Neural Crest Cells (2019) Methods Mol Biol 1879:307-321.. doi: 10.1007/7651_2018_145

AB-N01: Flow Cytometry

Wang Z, Wu J-L, Zhong F, Liu Y, Yu Y-Q, Sun J-J, Wang S, Li H, Zhou X-F, Hu Z-L, & Dai R-P. Upregulation of Probdnf in the Mesenteric Lymph Nodes in Septic Mice. (2019) Neurotox Res 36(3):540-550. doi: 10.1007/s12640-019-00081-3

AB-N01AP: Western (1:2000)

Zhong F, Liu L, Wei J-L, Hu Z-L, Li L, Wang S, Xu J-M, Zhou X-F, Li C-Q, Yang Z-Y, & Dai R-P. Brain-Derived Neurotrophic Factor Precursor in the Hippocampus Regulates Both Depressive and Anxiety-Like Behaviors in Rats. (2019) Front Psychiatry 9:776. doi: 10.3389/fpsyt.2018.00776

Objective: To investigate the role of proBDNF in the hippocampus in the development of depression and anxiety.
Summary: Despite some behavioral and morphological differences between depression and anxiety, hippocampal proBDNF is a common mediator to regulate these two mental disorders.
Application: Western Blot


Pottorf T, Mann A, Fross S, Mansel C, & Vohra BPS. Nicotinamide Mononucleotide Adenylyltransferase 2 maintains neuronal structural integrity through the maintenance of Golgi structure. (2018). Neurochem Intl

Objective:  investigated the link between NMNAT2 loss, Golgi fragmentation and axon degeneration.
Summary:  Results degeneration signals converge on a common cytNmnat1 mediated axon protective program and are distinct from the SARM1 mediated caspase independent axon degeneration.
Dose:  Immunohistochemistry (1:1000).

Virga DM, Capps J, & Vohra BPS. Enteric Neurodegeneration is Mediated Through Independent Neuritic and Somal Mechanisms in Rotenone and MPP+ Toxicity. (2018). Neurochem Res 1-16. 27 September 2018.

Objective:  To the mechanism by which enteric nervous system degeneration occurs in Parkinson’s Disease.
Summary:  Therapies targeting both axonal and somal protection can be important in finding interventions for enteric symptoms in PD.
Dose:  P75NTR antibody utilized (1:1000) to immunoselect enteric neural crest from the rest of the cells in the gut including stomach, small and large bowl and colon) from E12.5 Sprague CD1 mice.

Donnelly CR, Gabreski NA, Suh EB, Chowdhury M, & Pierchala BA. Non-canonical Ret signaling augments p75-mediated cell death in developing sympathetic neurons. (2018) J Cell Biol, 217 (9):327. DOI: 10.1083/jcb.201703120

Dose:  Proximity ligation assays (1:500); Western blotting (1:1,000)

Jones I, Novikova LN, Novikov LN, Renardy M, Ullrich A, Wiberg M, Carlsson L, Kingham PJ.  (2018)  Regenerative Effects of Human Embryonic Stem Cell-Derived Neural Crest Cells for Treatment of Peripheral Nerve Injury.  J Tissue Eng Regen Med  1-11.

Maass JC, Gu R, Cai T, Wan YW, Cantellano SC, Asprer JS, Zhang H, Jen HI, Edlund RK, Liu Z, Groves AK. (2016) Transcriptomic Analysis of Mouse Cochlear Supporting Cell Maturation Reveals Large-Scale Changes in Notch Responsiveness Prior to the Onset of Hearing. PLoS One 11(12):e0167286. PMID: 27918591 (Targeting Trends 17q1)

Wehner AB, Milen AM, Albin RL, Pierchala BA. (2016) The P75 Neurotrophin Receptor Augments Survival Signaling in the Striatum of Pre-Symptomatic Q175(wt/hd) Mice. Neuroscience 324:297-306. PMID: 26947127 (Targeting Trends 17q1)

Boisvert EM, Engle SJ, Hallowell SE, Liu P, Wang Z-W, & Li X-J. The Specification and Maturation of Nociceptive Neurons from Human Embryonic Stem Cells. (2015). SciRept, 5 16821. ICC  1:200

Gibon J, Buckley SM, Unsain N, Kaartinen V, Seguela P, Barker PA. (2015) proBDNF and p75NTR Control Excitability and Persistent Firing of Cortical Pyramidal Neurons. J Neurosci 35(26):9741-9753. PMID: 26134656 (Targeting Trends 16q1)

Burke RM, Norman TA, Haydar TF, Slack BE, Leeman SE, Blusztajn JK, Mellott TJ. (2013) BMP9 ameliorates amyloidosis and the cholinergic defect in a mouse model of Alzheimer’s disease. Proc Natl Acad Sci U S A 110(48):19567-19572. PMID: 24218590 (Targeting Trends 14q1)

Kinikoglu B, Kong Y, Liao EC. (2014) Featured Article: Characterization of cultured multipotent zebrafish neural crest cells. Exp Biol Med (Maywood) 239(2):159-168. PMID: 24326414 (Targeting Trends 14q1)

Girard BM, Malley SE, Vizzard MA. (2011) Neurotrophin/receptor expression in urinary bladder of mice with overexpression of NGF in urothelium. Am J Physiol Renal Physiol 300(2):F345-55. PMID: 21048026 (Targeting Trends 11q2)

Schnitzler AC, Mellott TJ, Lopez-Coviella I, Tallini YN, Kotlikoff MI, Follettie MT, Blusztajn JK. (2010) BMP9 (bone morphogenetic protein 9) induces NGF as an autocrine/paracrine cholinergic trophic factor in developing basal forebrain neurons. J Neurosci 30(24):8221-8228. PMID: 20554873 (Targeting Trends 10q3)

Rock JR, Onaitis MW, Rawlins EL, Lu Y, Clark CP, Xue Y, Randell SH, Hogan BL (2009) Basal cells as stem cells of the mouse trachea and human airway epithelium. Proc Natl Acad Sci U S A 106(31):12771-12775.

Watanabe T, Ito T, Inoue G, Ohtori S, Kitajo K, Doya H, Takahashi K, Yamashita T. (2008) The p75 receptor is associated with inflammatory thermal hypersensitivity. J Neurosci Res 86(16):3566-3574. PMID: 18709654 (Targeting Trends 14q2)

Lee G, Kim H, Elkabetz Y, Al Shamy G, Panagiotakos G, Barberi T, Tabar V, & Studer L. Isolation and directed differentiation of neural crest stem cells derived from human embryonic stem cells. (2007). Nat Biotechnol, 25 (12):1468-1475. 2007/11/27.

Flow Cytometry cell sorting was performed using Anti-NGFr (p75).

Singh B, Henneberger C, Betances D, Arevalo MA, Rodríguez-Tébar A, Meier JC, Grantyn R (2006) Altered balance of glutamatergic/GABAergic synaptic input and associated changes in dendrite morphology after BDNF expression in BDNF-deficient hippocampal neurons. J Neurosci 26(27):7189-7200.

Lopez-Coviella I, Follettie MT, Mellott TJ, Kovacheva VP, Slack BE, Diesl V, Berse B, Thies RS, Blusztajn JK (2005) Bone morphogenetic protein 9 induces the transcriptome of basal forebrain cholinergic neurons. Proc Natl Acad Sci U S A 102(19):6984-6989.

Pehar M, Cassina P, Vargas MR, Castellanos R, Viera L, Beckman JS, Estévez AG, & Barbeito L. Astrocytic Production of Nerve Growth Factor in Motor Neuron Apoptosis: Implications for Amyotrophic Lateral Sclerosis. (2004). Journal of Neurochemistry, 89 (2):464-473.

AB-N01: The role of p75-mediated activity evaluated with p75NTR blocking antibody.  Motor neuron loss induced by reactive astrocytes was prevented by blocking the p75NTR.

Campagnolo L, Russo MA, Puglianiello A, Favale A, Siracusa G (2001) Mesenchymal cell precursors of peritubular smooth muscle cells of the mouse testis can be identified by the presence of the p75 neurotrophin receptor. Biol Reprod 64(2):464-472.

Bannerman P, Nichols W, Puhalla S, Oliver T, Berman M, Pleasure D (2000) Early migratory rat neural crest cells express functional gap junctions: Evidence that neural crest cell survival requires gap junction function. J Neurosci Res 61:605-615. (Targeting Trends 01q2)

Huber LJ, Chao MV (1995) Mesenchymal and neuronal cell expression of the p75 neurotrophin receptor gene occur by different mechanisms. Devel Biol 167:227-238.

Huber LJ, Lee K-F, Dreyfus CF, Chao MV (1994) Generation and characterization of a murine p75 receptor blocking antibody. Soc Neurosci Mtg, Miami Beach FL, Abstract #23-12.

Hempstead BL, Martin-Zanca D, Kaplan DR, Parada LF, Chao MV (1991) High-affinity NGF binding requires coexpression of the trk proto-oncogene and the low-affinity NGF receptor. Nature 350(6320):678-683.