Corticotropin Releasing Hormone Rabbit Polyclonal (Cat. #AB-02)
18 entries found for : ab-02
Amano M, Amiya N, Fukushima K, Hagio H, Yamamoto N, Sakakura Y (2022) Effects of crowding stress on the hypothalamo-pituitary-interrenal axis of the self-fertilizing fish, Kryptolebias marmoratus. Comp Biochem Physiol A Mol Integr Physiol 264:111110. doi: 10.1016/j.cbpa.2021.111110
Objective: To test whether crowding stress affects the hypothalamo-pituitary-interrenal (HPI) axis of the self-fertilizing fish, Kryptolebias marmoratus.
Summary: The number of CRH-ir cell bodies and cortisol levels were significantly higher in paired fish. The results indicate that crowding stress affects the HPI axis in K. marmoratus which thrive in small burrows with limited water volume.
Usage: Immunohistochemistry (1:2000)
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Li X (2019) Light stimulation into dorsal raphe nucleus contributes to antidepressant effect for a stressed rat model. bioRxiv 821421. doi: 10.1101/821421Usage: immunohisochemistry (1:500)
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Enteric apelin enhances the stress-induced stimulation of colonic motor functions.
Bülbül M, Sinen O, Bayramoğlu O, Akkoyunlu G (2019) Enteric apelin enhances the stress-induced stimulation of colonic motor functions. Stress 23(2):201-212. doi: 10.1080/10253890.2019.1658739Usage: histology (1:100)
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Xu T, Zhou Z, Liu N, Deng C, Huang G, Zhou F, Liu X, Wang X (2019) Disrupted compensatory response mediated by Wolfram syndrome 1 protein and corticotrophin-releasing hormone family peptides in early-onset intrahepatic cholestasis pregnancy. Placenta 83:63-71. doi: 10.1016/j.placenta.2019.06.378Usage: Western (1:1000)
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de Miguel E, Vekovischeva O, Elsilä LV, Panhelainen A, Kankuri E, Aitta-aho T, Korpi ER (2019) Conditioned aversion and neuroplasticity induced by a superagonist of extrasynaptic gabaa receptors: Correlation with activation of the oval BNST neurons and CRF mechanisms. Front Mol Neurosci 12:130. doi: 10.3389/fnmol.2019.00130Usage: immunohistochemistry (1:250)
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Amano M, Amiya N, Yamamoto N, Osugi T, Tsutsui K (2019) Immunohistochemical detection of prolactin-releasing peptide2 in the brain of the inshore hagfish Eptatretus burgeri. Gen Comp Endocrinol 274:1-7. doi: 10.1016/j.ygcen.2018.12.005Objective: To identify Prolactin-releasing peptide2 (PrRP2) in hagfish.
Summary: The study demonstrates, for the first time, that PrRP2 is expressed in the brain of inshore hagfish. The restricted distribution of PrRP2-ir fibers in the HYinf suggests that PrRP2 does not directly regulate the pituitary gland, but regulates the function of the HYinf where PQRFa and CRH are expressed.
Usage: Immunohistochemistry (1:1000). The authors clarified the cross-reactivity of the antibody against other CRH family peptides, such as urocortin-I, II, III, urotensin-I, and sauvagine: the cross-reactivity of anti-CRH against CRH family peptides was found to be less than 0.01%, indicating the specificity of the antibody.
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Ke Q, Li R, Cai L, Wu SD, Li CM (2019) Ro41-5253, a selective antagonist of retinoic acid receptor α, ameliorates chronic unpredictable mild stress-induced depressive-like behaviors in rats: Involvement of regulating HPA axis and improving hippocampal neuronal deficits. Brain Res Bull 146:302-309. doi: 10.1016/j.brainresbull.2019.01.022Usage: histology
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Wang Y, Li G, Wang X, Zhu S (2019) Effects of Shugan Hewei Granule on depressive behavior and protein expression related to visceral sensitivity in a rat model of nonerosive reflux disease. Evid Based Complement Alternat Med 2019:1505693. doi: 10.1155/2019/1505693Usage: immunofluorescent staining (1:200)
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Tao X, Li N, Liu F, Hu Y, Liu J, Zhang Y-M (2018) In vitro examination of microglia-neuron crosstalk with BV2 cells, and primary cultures of glia and hypothalamic neurons. Heliyon 4:e00730. doi: 10.1016/j.heliyon.2018.e00730
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Trifunovic S, Manojlovic-Stojanoski M, Nestorovic N, Ristic N, Sosic-Jurjevic B, Pendovski L, Milosevic V (2018) Histological and morphofunctional parameters of the hypothalamic-pituitary-adrenal system are sensitive to daidzein treatment in the adult rat. Acta Histochem 120:129-135. doi: 10.1016/j.acthis.2017.12.006 Related Products: Corticotropin Releasing Hormone Rabbit Polyclonal (Cat. #AB-02)
Amano M, Amiya N, Yokoyama T, Onikubo K, Yamamoto N, Takahashi A (2016) Immunohistochemical detection of corticotropin-releasing hormone (CRH) in the brain and pituitary of the hagfish, Eptatretus burgeri. Gen Comp Endocrinol 236:174-180. doi: 10.1016/j.ygcen.2016.07.018
Summary: The distribution of corticotropin-releasing hormone (CRH) in the brain and pituitary of the hagfish Eptatretus burgeri, representing the earliest branch of vertebrates, was examined by immunohistochemistry to better understand the neuroendocrine system of hagfish. A rabbit polyclonal antibody raised against human/mouse/rat CRH (Cat. #AB-02) was used. A standard curve was obtained from 0.78 ng/ml to 50 ng/ml. The cross-reactivity of anti-CRH antibody against CRH family peptides was found to be less than 0.01%, indicating the specificity of the antibody. The specificity of the antibody raised against human/mouse/rat CRH was demonstrated by a TR-FIA and absorption test. CRH-ir cell bodies were detected in two brain regions; the preopticohypothalamic area (PO, POp, and Hyinf) and the medulla oblongata. CRH-ir fibers were mainly distributed in the hypothalamus and the medulla oblongata, in which CRH-ir cell bodies were detected.
Related Products: Corticotropin Releasing Hormone Rabbit Polyclonal (Cat. #AB-02)
Wagle M, Mathur P, Guo S (2011) Corticotropin-releasing factor critical for zebrafish camouflage behavior is regulated by light and sensitive to ethanol. J Neurosci 31(1):214-224. doi: 10.1523/JNEUROSCI.3339-10.2011
Objective: To explore neural circuit assembly and function using the hardwired camouflage response of zebrafish.
Summary: Both light exposure and ethanol affect the camouflage response. Understanding this system could provide a tool to further investigate the effect of alcohol on neural circuits.
Usage: Immunostaining (1:500)
Related Products: Corticotropin Releasing Hormone Rabbit Polyclonal (Cat. #AB-02)
Oxytocin deficiency mediates hyperphagic obesity of Sim1 haploinsufficient mice.
Kublaoui BM, Gemelli T, Tolson KP, Wang Y, Zinn AR (2008) Oxytocin deficiency mediates hyperphagic obesity of Sim1 haploinsufficient mice. Mol Endocrinol 22(7):1723-1734. doi: 10.1210/me.2008-0067
Summary: Central administration of neuropeptides in the paraventricular nucleus (PVN) is known to inhibit feeding. Here the authors examined altered hypothalamic expression of PVN neuropeptides in Sim1+/- mice. Hypothalamic expression of several neuropeptides, including corticotrophin releasing hormone (Crh) was measured. To do so, anti-Crh (Cat. #AB-02, 1:800) was used in immunohistochemistry. The data suggests that these neurons are involved in melanocortin feeding circuits.
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Xu J, Xu F, Hennebold JD, Molskness TA, Stouffer RL (2007) Expression and role of the corticotropin-releasing hormone/urocortin-receptor-binding protein system in the primate corpus luteum during the menstrual cycle. Endocrinology 148(11):5385-5395. doi: 10.1210/en.2007-0541
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Anton PM, Gay J, Mykoniatis A, Pan A, O'Brien M, Brown D, Karalis K, Pothoulakis C (2004) Corticotropin-releasing hormone (CRH) requirement in Clostridium difficile toxin A-mediated intestinal inflammation. Proc Natl Acad Sci U S A 101(22):8503-8508. doi: 10.1073/pnas.0402693101
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Widmaier EP, Lim AT, Vale W (1989) Secretion of corticotropin-releasing factor from cultured rat hypothalamic cells: effects of catecholamines. Endocrinology 124:583-590. doi: 10.1210/endo-124-2-583
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Bloch B, Gaillard RC, Brazeau P, Lin HD, Ling N (1984) Topographical and ontogenetic study of the neurons producing growth hormone-releasing factor in human hypothalamus. Regul Pept 8(1):21-31. doi: 10.1016/0167-0115(84)90025-9
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Assay of corticotropin releasing factor.
Vale W, Vaughan J, Yamamoto G, Bruhn T, Douglas C, Dalton D, Rivier C, Rivier J (1983) Assay of corticotropin releasing factor. In: Methods in Enzymology 103:565-577. Elsevier. doi: 10.1016/s0076-6879(83)03040-2
Related Products: Corticotropin Releasing Hormone Rabbit Polyclonal (Cat. #AB-02)