vGAT References

Cat. #AB-N44


Friedman CA, Russell BJ, Kohls MD, Ancheta LR, Shramm PA, Lappi DA (2016) Targeting vesicular gaba transporter (vGAT)-expressing cells with a polyclonal antibody to the lumenal domain of vGAT: results with a saporin conjugate. Soc Neurosci Meeting Abstract 124.06


Gertow K, Wolbank S, Rozell B, Sugars R, Andang M, Parish CL, Imreh MP, Wendel M, & Ahrlund-Richter L. Organized Development from Human Embryonic Stem Cells after Injection into Immunodeficient Mice. (2004). Stem Cells Dev, 13 (4):421-435. 2004/09/04.

After thawing and brief drying, sections were fixed with 4% paraformaldehyde in PBS pH 7.4 for 40 min.  Sections were blocked [PBS, 1% bovine serum albumin (BSA), 0.3% Triton X-100], washed, and incubated with primary antibodies for Tuj1 (-tubulin type III), glial fibrillary acid protein (GFAP), tyrosine hydroxylase (TH), and vesicular GABA transporter (vGAT; 1:400) overnight at 4°C.  After rinsing in PBS, slides were incubated with Cy2- or Cy3-conjugated secondary antibodies.