Meng J, Chen W, & Wang J. Intervening B-Type Natriuretic Peptide Signaling for Controlling Chronic Itch. (2019). Brit J Pharmacol, (in press)
Objective: Review of recent findings used to examine the role of B-type natriuretic peptide (BNP) in itch transduction and the modulation of other pururitic proteims.
Summary: Mice treated with Nppb-saporin, a toxin ablated 70% BNP receptor positive neurons in the spinal cord (Mishra & Hoon, 2013; Pitake, Ralph, DeBrecht & Mishra, 2018).
Sakata D, Uruno T, Matsubara K, Andoh T, Yamamura K, Magoshi Y, Kunimura K, Kamikaseda Y, Furue M, & Fukui Y. Selective role of neurokinin B in IL-31–induced itch response in mice. (2019). J Allergy Clin Immunol, [Epub ahead of print]
IT-69: Nppb-SAP; IT-40: Bombesin-SAP; IT-21: Blank-SAP
Also see IT-63: NK3-SAP
Objective: To examine the physiological significance of neurokinin B in IL-31–induced itch sensation.
Summary: IL-31–induced scratching was unaffected by intrathecal injection of Nppb-SAP. In contrast, treatment with Bombesin-SAP reduced IL-31–induced scratching. Neurokinin B acts upstream of GRP to transmit IL-31–induced itch sensation.
Dose: Intrathecal injection.
Fatima M, Ren X, Pan H, Slade HFE, Asmar AJ, Xiong CM, Shi A, Xiong AE, Wang L, & Duan B. Spinal somatostatin-positive interneurons transmit chemical itch. (2019). PAIN, 160 (5):
Objective: To further study the cellular identity of spinal interneurons that contribute to itch processing.
Summary: Findings reveal a novel spinal mechanism for sensory encoding of itch perception.
Dose: Npra receptor–expressing spinal cord interneurons were ablated through intrathecal injection of Nppb-SAP (5 μg/10 μL) or control Blank-SAP in lumbar segment 3 to 4. Behavioral analyses were performed 1 week after the toxin injection.
Pitake S, Ralph PC, DeBrecht J, & Mishra SK. Atopic Dermatitis Linked Cytokine Interleukin-31 Induced Itch Mediated via a Neuropeptide Natriuretic Polypeptide B. (2018). Acta Derm Venereol, 98 (8):795-796. 2018/05/26.
Objective: To determine if NPPB is involved as a neuropeptide in IL-31-mediated itch in atopic dermatitis (AD) via natriuretic polypeptide receptor A (NPRA) in the spinal cord.
Summary: This study reveals an important role of neuropeptide NPPB in AD that could provide a therapeutic target for alleviating chronic itch associated with AD.
Dose: To further demonstrate the IL-31-mediated itch response by NPRA receptors expressed in the spinal cord, Nppb-SAP (5 μg) was used to eliminate neurons expressing NPRA receptors in the spinal cord.
Huang J, Polgár E, Solinski HJ, Mishra SK, Tseng P-Y, Iwagaki N, Boyle KA, Dickie AC, Kriegbaum MC, Wildner H, Zeilhofer HU, Watanabe M, Riddell JS, Todd AJ, & Hoon MA. Circuit Dissection of the Role of Somatostatin in Itch and Pain. (2018). Nature Neuroscience.
Objective: To determine the role of somatostatin in itch and pain.
Summary: Results define the neural circuit underlying somatostatin-induced itch and characterize a contrasting antinociceptive role for the peptide.
Dose: Ablation of Npr1- and GRPR-expressing spinal cord interneurons was accomplished by intrathecal (segment L3/4) injection of Nppb-SAP (4 μg/10 μL) and Bombesin-SAP (2.5 μg) respectively.
Mishra SK, Hoon MA. (2013) The cells and circuitry for itch responses in mice. Science 340(6135):968-971. (Targeting Trends 13q3)