NK3-SAP References

Cat #IT-63

Goodman RL, He W, Lopez JA, Bedenbaugh MN, McCosh RB, Bowdridge EC, Coolen LM, Lehman MN, & Hileman SM. Evidence That the LH Surge in Ewes Involves Both Neurokinin B–Dependent and –Independent Actions of Kisspeptin. (2019) Endocrinology, 160 (12):2990-3000. . doi: 10.1210/en.2019-00597

Objective:  To determine if NKB is involved in the RCh of the ewe in the LH surge.
Summary:  NKB signaling in the RCh increases kisspeptin levels critical for the full amplitude of the LH surge in the ewe, but kisspeptin release occurs independently of retrochiamatic area (RCh) input at the onset of the surge to initiate GnRH secretion.
Dose:  Bilaterial injections in the RCh of either NK3-SAP or Blank-SAP.

Krajewski-Hall SJ, Miranda Dos Santos F, McMullen NT, Blackmore EM, Rance NE. Glutamatergic Neurokinin 3 Receptor Neurons in the Median Preoptic Nucleus Modulate Heat-Defense Pathways in Female Mice. (2019) Endocrinology 160(4):803-816. doi: 10.1210/en.2018-00934

Objective: To characterize the thermoregulatory role of MnPO NK3R neurons in female mice.
Summary: Study suggests that KNDy neurons modulate thermosensory pathways for heat defense indirectly via a subpopulation of glutamatergic MnPO neurons that express NK3R.
Dose: Mice were bilaterally injected with 10 ng NK3-SAP in 100 nL PBS (n = 14) or blank-SAP (n = 8) in the preoptic area adjacent to the MnPO.

Sakata D, Uruno T, Matsubara K, Andoh T, Yamamura K, Magoshi Y, Kunimura K, Kamikaseda Y, Furue M, & Fukui Y. Selective role of neurokinin B in IL-31–induced itch response in mice. (2019) J Allergy Clin Immunol, 144 (4):1130-1133. doi: 10.1016/j.jaci.2019.06.031

Objective:  To examine the physiological significance of neurokinin B in IL-31–induced itch sensation. Summary:  IL-31–induced scratching was unaffected by intrathecal injection of Nppb-SAP.  In contrast, treatment with Bombesin-SAP reduced IL-31–induced scratching.  Neurokinin B acts upstream of GRP to transmit IL-31–induced itch sensation.
Dose: Intrathecal injection.

Mittelman-Smith MA, Krajewski-Hall SJ, McMullen NT, Rance NE. (2016) Ablation of KNDy neurons results in hypogonadotropic hypogonadism and amplifies the steroid-induced LH surge in female rats. Endocrinology 157(5):2015-2027. PMID: 26937713 (Targeting Trends 16q3)

Helena CV, Toporikova N, Kalil B, Stathopoulos AM, Pogrebna VV, Carolino RO, Anselmo-Franci JA, Bertram R. (2015) KNDy neurons modulate the magnitude of the steroid-induced luteinizing hormone surges in ovariectomized rats. Endocrinology 156(11):4200-4213. PMID: 26302111 (Targeting Trends 15q4)

Mittelman-Smith MA, Krajewski-Hall SJ, McMullen NT, Rance NE. (2015) Neurokinin 3 Receptor-Expressing Neurons in the Median Preoptic Nucleus Modulate Heat-Dissipation Effectors in the Female Rat. Endocrinology 156(7):2552-2562. (Targeting Trends 15q3)

Helena CV, Kalil B, Anselmo-Franci JA, Bertram R. (2013) Time Course Study of Targeted Ablation of KNDy Neurons, but not Tyrosine-Hydroxylase Neurons, in the Rat Arcuate Nucleus Using a Neurokinin B-Saporin. Endocr Rev 34 (03_MeetingAbstracts):OR47-5. (Targeting Trends 13q3)

Mittelman-Smith MA, Williams H, Krajewski-Hall SJ, Lai J, Ciofi P, McMullen NT, Rance NE. (2012) Arcuate kisspeptin/neurokinin B/dynorphin (KNDy) neurons mediate the estrogen suppression of gonadotropin secretion and body weight. Endocrinology 153(6):2800-2812. (Targeting Trends 12q2)

Mittelman-Smith MA, Williams H, Krajewski-Hall SJ, McMullen NT, Rance NE. (2012) Role for kisspeptin/neurokinin B/dynorphin (KNDy) neurons in cutaneous vasodilatation and the estrogen modulation of body temperature. Proc Natl Acad Sci U S A 109(48):19846-19851. (Targeting Trends 13q1)

Rance NE, Mittelman-Smith MA, Krajewski-Hall SJ. (2012) Use of a novel saporin conjugate (NK3-SAP) to study the function of neurokinin 3 receptor (NK3r)-expressing kisspeptin/neurokinin B/dynorphin (KNDy) neurons in the rat arcuate nucleus. Targeting Trends 13(2).

Corboz MR, Rivelli MA, Eckel SP (2010) Bronchoconstrictor effect of the tachykinin NK(3)-receptor agonists [MePhe(7)]-neurokinin B and senktide in the isolated guinea pig lung. Exp Lung Res 36:509-521.

Drapeau G, d’Orléans-Juste P, Dion S, Rhaleb N-E, Regoli D (1987) Selective agonists for substance P and neurokinin receptors. Neuropeptides 10:43-54.

Drapeau G, d’Orléans-Juste P, Dion S, Rhaleb N-E, Regoli D (1987) Specific agonists for neurokinin B receptors. Eur J Pharmacol 136:401-403.