Oxytocin-SAP References

Cat #IT-46

Oti T, Satoh K, Uta D, Nagafuchi J, Tateishi S, Ueda R, Takanami K, Young LJ, Galione A, Morris JF, Sakamoto T, Sakamoto H. Oxytocin Influences Male Sexual Activity via Non-synaptic Axonal Release in the Spinal Cord. (2021) Curr Biol 31(1):103-114.e5. doi: 10.1016/j.cub.2020.09.089

KIT-46:  Oxytocin-SAP and Blank-SAP
Summary:  Oxytocin directly activates SEG (Spinal Ejaculation Generator)/GRP (Gastrin-Releasing Peptide) neurons via OXTRs (Oxytocin Receptors) and influences male sexual function in the rat lumbar spinal cord.
Dose:  Oxytocin-SAP  (4 and 40 ng ml–1) was microinjected slowly as a 1 ml injection into the midline at 1 to 1.5 mm below the dorsal surface of the spinal cord from L3 to L4. Blank-SAP was used as control.

Kohli S, King MV, Williams S, Edwards A, Ballard TM, Steward LJ, Alberati D, Fone KCF. Oxytocin attenuates phencyclidine hyperactivity and increases social interaction and nucleus accumben dopamine release in rats. (2019) Neuropsychopharmacology 44(2):295-305. doi: 10.1038/s41386-018-0171-0

Summary:  The authors suggest that further evaluation such as by microinjection with selective antagonists or lesions using the neurotoxin, Oxytocin-SAP, would help delineate the brain region/s and receptor/s involved.

Angioni L, Cocco C, Ferri GL, Argiolas A, Melis MR, Sanna F. (2016) Involvement of nigral oxytocin in locomotor activity: A behavioral, immunohistochemical and lesion study in male rats. Horm Behav 83:23-38. PMID: 27189764 (Targeting Trends 16q3)

Matsuura T, Kawasaki M, Hashimoto H, Yoshimura M, Motojima Y, Saito R, Ueno H, Maruyama T, Ishikura T, Sabanai K, Mori T, Ohnishi H, Onaka T, Sakai A, Ueta Y. (2016) Possible Involvement of the Rat Hypothalamo-Neurohypophysial/-Spinal Oxytocinergic Pathways in Acute Nociceptive Responses. J Neuroendocrinol 28(6) PMID: 27144381 (Targeting Trends 16q3)

Matsuura T, Kawasaki M, Hashimoto H, Yoshimura M, Motojima Y, Saito R, Ueno H, Maruyama T, Sabanai K, Mori T, Ohnishi H, Sakai A, Ueta Y. (2016) Effects of central administration of oxytocin-saporin cytotoxin on chronic inflammation and feeding/drinking behaviors in adjuvant arthritic rats. Neurosci Lett 621:104-110. PMID: 27060190 (Targeting Trends 16q3)

Baskin DG, Kim F, Gelling RW, Russell BJ, Schwartz MW, Morton GJ, Simhan HN, Moralejo DH, Blevins JE (2010) A new oxytocin-saporin cytotoxin for lesioning oxytocin-receptive neurons in the rat hindbrain. Endocrinology 151(9):4207-4213. (Targeting Trends 10q4)